Amelioration of ultraviolet-induced photokeratitis in mice treated with astaxanthin eye drops

Anton Lennikov, Nobuyoshi Kitaichi, Risa Fukase, Miyuki Murata, Kousuke Noda, Ryo Ando, Takeshi Ohguchi, Tetsuya Kawakita, Shigeaki Ohno, Susumu Ishida

Research output: Contribution to journalArticle

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Abstract

Purpose: Ultraviolet (UV) acts as low-dose ionizing radiation. Acute UVB exposure causes photokeratitis and induces apoptosis in corneal cells. Astaxanthin (AST) is a carotenoid, present in seafood, that has potential clinical applications due to its high antioxidant activity. In the present study, we examined whether topical administration of AST has preventive and therapeutic effects on UV-photokeratitis in mice. Methods: C57BL/6 mice were administered with AST diluted in polyethylene glycol (PEG) in instillation form (15 μl) to the right eye. Left eyes were given vehicle alone as controls. Immediately after the instillation, the mice, under anesthesia, were irradiated with UVB at a dose of 400 mJ/cm 2. Eyeballs were collected 24 h after irradiation and stained with H&E and TUNEL. In an in vitro study, mouse corneal epithelial (TKE2) cells were cultured with AST before UV exposure to quantify the UV-derived cytotoxicity. Results: UVB exposure induced cell death and thinning of the corneal epithelium. However, the epithelium was morphologically well preserved after irradiation in AST-treated corneas. Irradiated corneal epithelium was significantly thicker in eyes treated with AST eye drops, compared to those treated with vehicles (p<0.01), in a doses dependent manner. Significantly fewer apoptotic cells were observed in AST-treated eyes than controls after irradiation (p<0.01). AST also reduced oxidative stress in irradiated corneas. The in vitro study showed less cytotoxicity of TKE2 cells in AST-treated cultures after UVB-irradiation (p<0.01). The cytoprotective effect increased with the dose of AST. Conclusions: Topical AST administration may be a candidate treatment to limit the damages by UV irradiation with wide clinical applications.

Original languageEnglish
Article numbera49
Pages (from-to)455-464
Number of pages10
JournalMolecular Vision
Volume18
Publication statusPublished - 2012 Feb 14

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Ophthalmic Solutions
Topical Administration
Corneal Epithelium
Cornea
astaxanthine
Seafood
In Situ Nick-End Labeling
Therapeutic Uses
Carotenoids
Ionizing Radiation
Inbred C57BL Mouse
Oxidative Stress
Cell Death
Epithelium
Anesthesia
Antioxidants
Epithelial Cells
Apoptosis

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Lennikov, A., Kitaichi, N., Fukase, R., Murata, M., Noda, K., Ando, R., ... Ishida, S. (2012). Amelioration of ultraviolet-induced photokeratitis in mice treated with astaxanthin eye drops. Molecular Vision, 18, 455-464. [a49].

Amelioration of ultraviolet-induced photokeratitis in mice treated with astaxanthin eye drops. / Lennikov, Anton; Kitaichi, Nobuyoshi; Fukase, Risa; Murata, Miyuki; Noda, Kousuke; Ando, Ryo; Ohguchi, Takeshi; Kawakita, Tetsuya; Ohno, Shigeaki; Ishida, Susumu.

In: Molecular Vision, Vol. 18, a49, 14.02.2012, p. 455-464.

Research output: Contribution to journalArticle

Lennikov, A, Kitaichi, N, Fukase, R, Murata, M, Noda, K, Ando, R, Ohguchi, T, Kawakita, T, Ohno, S & Ishida, S 2012, 'Amelioration of ultraviolet-induced photokeratitis in mice treated with astaxanthin eye drops', Molecular Vision, vol. 18, a49, pp. 455-464.
Lennikov A, Kitaichi N, Fukase R, Murata M, Noda K, Ando R et al. Amelioration of ultraviolet-induced photokeratitis in mice treated with astaxanthin eye drops. Molecular Vision. 2012 Feb 14;18:455-464. a49.
Lennikov, Anton ; Kitaichi, Nobuyoshi ; Fukase, Risa ; Murata, Miyuki ; Noda, Kousuke ; Ando, Ryo ; Ohguchi, Takeshi ; Kawakita, Tetsuya ; Ohno, Shigeaki ; Ishida, Susumu. / Amelioration of ultraviolet-induced photokeratitis in mice treated with astaxanthin eye drops. In: Molecular Vision. 2012 ; Vol. 18. pp. 455-464.
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AU - Fukase, Risa

AU - Murata, Miyuki

AU - Noda, Kousuke

AU - Ando, Ryo

AU - Ohguchi, Takeshi

AU - Kawakita, Tetsuya

AU - Ohno, Shigeaki

AU - Ishida, Susumu

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