Amino acid analysis by capillary electrophoresis-mass spectrometry

Akiyoshi Hirayama, Tomoyoshi Soga

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Abstract

A method for the determination of underivatized amino acids based on capillary electrophoresis-mass spectrometry is described. To analyze free amino acids simultaneously, a low acidic pH electrolyte was used to confer positive charge on whole amino acids. All protonated amino acids migrated toward the cathode in CE and then were detected by electrospray ionization mass spectrometry with high selectivity and sensitivity. This method is simple, rapid, and selective and could be readily applied to the analysis of free amino acids in various samples.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages77-82
Number of pages6
Volume828
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume828
ISSN (Print)10643745

Fingerprint

Capillary Electrophoresis
Mass Spectrometry
Amino Acids
Electrospray Ionization Mass Spectrometry
Electrolytes
Electrodes

Keywords

  • Amino acid analysis
  • Capillary electrophoresis
  • Mass spectrometry
  • Quadrupole mass spectrometry
  • Time-of-flight mass spectrometry
  • Triple-quadrupole tandem mass spectrometry
  • Tumor tissue

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hirayama, A., & Soga, T. (2012). Amino acid analysis by capillary electrophoresis-mass spectrometry. In Methods in Molecular Biology (Vol. 828, pp. 77-82). (Methods in Molecular Biology; Vol. 828). https://doi.org/10.1007/978-1-61779-445-2_8

Amino acid analysis by capillary electrophoresis-mass spectrometry. / Hirayama, Akiyoshi; Soga, Tomoyoshi.

Methods in Molecular Biology. Vol. 828 2012. p. 77-82 (Methods in Molecular Biology; Vol. 828).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hirayama, A & Soga, T 2012, Amino acid analysis by capillary electrophoresis-mass spectrometry. in Methods in Molecular Biology. vol. 828, Methods in Molecular Biology, vol. 828, pp. 77-82. https://doi.org/10.1007/978-1-61779-445-2_8
Hirayama A, Soga T. Amino acid analysis by capillary electrophoresis-mass spectrometry. In Methods in Molecular Biology. Vol. 828. 2012. p. 77-82. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-445-2_8
Hirayama, Akiyoshi ; Soga, Tomoyoshi. / Amino acid analysis by capillary electrophoresis-mass spectrometry. Methods in Molecular Biology. Vol. 828 2012. pp. 77-82 (Methods in Molecular Biology).
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