Amino acid analysis by capillary electrophoresis-mass spectrometry

Akiyoshi Hirayama, Tomoyoshi Soga

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Abstract

A method for the determination of underivatized amino acids based on capillary electrophoresis-mass spectrometry is described. To analyze free amino acids simultaneously, a low acidic pH electrolyte was used to confer positive charge on whole amino acids. All protonated amino acids migrated toward the cathode in CE and then were detected by electrospray ionization mass spectrometry with high selectivity and sensitivity. This method is simple, rapid, and selective and could be readily applied to the analysis of free amino acids in various samples.

Original languageEnglish
Title of host publicationAmino Acid Analysis
Subtitle of host publicationMethods and Protocols
EditorsMichail Alterman, Peter Hunziker
Pages77-82
Number of pages6
DOIs
Publication statusPublished - 2012 Jan 2

Publication series

NameMethods in Molecular Biology
Volume828
ISSN (Print)1064-3745

Keywords

  • Amino acid analysis
  • Capillary electrophoresis
  • Mass spectrometry
  • Quadrupole mass spectrometry
  • Time-of-flight mass spectrometry
  • Triple-quadrupole tandem mass spectrometry
  • Tumor tissue

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Hirayama, A., & Soga, T. (2012). Amino acid analysis by capillary electrophoresis-mass spectrometry. In M. Alterman, & P. Hunziker (Eds.), Amino Acid Analysis: Methods and Protocols (pp. 77-82). (Methods in Molecular Biology; Vol. 828). https://doi.org/10.1007/978-1-61779-445-2_8