Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry

Akiyoshi Hirayama, Satsuki Ikeda, Asako Sato, Tomoyoshi Soga

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages307-313
Number of pages7
DOIs
Publication statusPublished - 2019 Jan 1

Publication series

NameMethods in Molecular Biology
Volume2030
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Capillary electrophoresis
  • Mass spectrometry
  • Tandem mass spectrometry
  • Tumor tissue

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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