Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry

Akiyoshi Hirayama, Satsuki Ikeda, Asako Sato, Tomoyoshi Soga

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages307-313
Number of pages7
DOIs
Publication statusPublished - 2019 Jan 1

Publication series

NameMethods in Molecular Biology
Volume2030
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Capillary Electrophoresis
Mass Spectrometry
Amino Acids
Tandem Mass Spectrometry
Electrolytes
Electrodes

Keywords

  • Capillary electrophoresis
  • Mass spectrometry
  • Tandem mass spectrometry
  • Tumor tissue

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hirayama, A., Ikeda, S., Sato, A., & Soga, T. (2019). Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry. In Methods in Molecular Biology (pp. 307-313). (Methods in Molecular Biology; Vol. 2030). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9639-1_23

Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry. / Hirayama, Akiyoshi; Ikeda, Satsuki; Sato, Asako; Soga, Tomoyoshi.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 307-313 (Methods in Molecular Biology; Vol. 2030).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hirayama, A, Ikeda, S, Sato, A & Soga, T 2019, Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2030, Humana Press Inc., pp. 307-313. https://doi.org/10.1007/978-1-4939-9639-1_23
Hirayama A, Ikeda S, Sato A, Soga T. Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 307-313. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9639-1_23
Hirayama, Akiyoshi ; Ikeda, Satsuki ; Sato, Asako ; Soga, Tomoyoshi. / Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 307-313 (Methods in Molecular Biology).
@inbook{c3fe110bd6b544f7988a2f011f636eea,
title = "Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry",
abstract = "Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.",
keywords = "Capillary electrophoresis, Mass spectrometry, Tandem mass spectrometry, Tumor tissue",
author = "Akiyoshi Hirayama and Satsuki Ikeda and Asako Sato and Tomoyoshi Soga",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-9639-1_23",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "307--313",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry

AU - Hirayama, Akiyoshi

AU - Ikeda, Satsuki

AU - Sato, Asako

AU - Soga, Tomoyoshi

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

AB - Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

KW - Capillary electrophoresis

KW - Mass spectrometry

KW - Tandem mass spectrometry

KW - Tumor tissue

UR - http://www.scopus.com/inward/record.url?scp=85070562772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070562772&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9639-1_23

DO - 10.1007/978-1-4939-9639-1_23

M3 - Chapter

C2 - 31347127

AN - SCOPUS:85070562772

T3 - Methods in Molecular Biology

SP - 307

EP - 313

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -