Amounts of proteins altered by mutations in the dnaA gene of Escherichia coli

Akiko Ohba, Tohru Mizushima, Tsutomu Katayama, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We identified proteins whose amounts were altered in a temperature-sensitive dnaA46 mutant of Escherichia coli. Proteins whose amounts were increased in the mutant were serine hydroxymethyltransferase, β-ketoacyl [acyl carrier protein] synthase II, long-chain fatty acid transport protein, and UDP-glucose 4-epimerase, while the decreased ones were flagellin and D-ribose-binding protein. Transformation of the mutant with a plasmid containing the wild type dnaA gene complemented the phenotype. As pulse-labeling experiments revealed that the rates of synthesis of the proteins were altered in the mutant, DnaA protein may be involved in expression of these proteins.

Original languageEnglish
Pages (from-to)125-128
Number of pages4
JournalFEBS Letters
Volume404
Issue number2-3
DOIs
Publication statusPublished - 1997 Mar 10

Keywords

  • DnaA protein
  • Escherichia coli
  • Gene expression
  • Two-dimensional polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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