Amplification of suppressor inducer pathway with monoclonal antibody, anti-2H4, identifying a novel epitope of the common leukocyte antigen/T200 antigen

Tsutomu Takeuchi, Christopher E. Rudd, Thomas F. Tedder, Stuart F. Schlossman, Chikao Morimoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The 2H4 antigen, comprised of a 200 220-kDa glycoprotein of the leukocyte common antigen (LCA) family, is expressed on a suppressor inducer, but not a helper inducer subset of T4 cells. Earlier studies have demonstrated that the T4+2H4+ subset of cells maximally responded to the AMLR and this molecule had an important role in generated suppressor signals in AMLR/ Con A-activated T cell systems. In the present study, we examined the effect of a series of monoclonal antibodies including anti-2H4 antibody on the initial activation of T4 cells in response to self-Ia antigens. We found that the addition of anti-2H4 antibody resulted in an augmentation of the proliferative response of T4 cells in AMLR, whereas other antibodies reactive with LCA/ T200 antigens lacked this ability. Furthermore, anti-2H4 antibody enhanced both IL-2 production and IL-2R expression in this AMLR system. This enhancing effect was inhibited by anti-T3 antibody. Moreover, the suppressor inducer function of AMLR T4 cells was enhanced with anti-2H4 antibody by increasing the number of 2H4+ cells with high antigen density. Taken together, these results suggest that the 2H4 antigen may serve as an accessory structure for enhancing the activation of the T4+2H4+ suppressor inducer subset at initiation of cell triggering.

Original languageEnglish
Pages (from-to)68-84
Number of pages17
JournalCellular Immunology
Volume118
Issue number1
DOIs
Publication statusPublished - 1989
Externally publishedYes

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CD45 Antigens
CD4-Positive T-Lymphocytes
Epitopes
Anti-Idiotypic Antibodies
Monoclonal Antibodies
Histocompatibility Antigens Class II
Autoantigens
Interleukin-2
Glycoproteins
Cell Count
T-Lymphocytes
Antigens
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Amplification of suppressor inducer pathway with monoclonal antibody, anti-2H4, identifying a novel epitope of the common leukocyte antigen/T200 antigen. / Takeuchi, Tsutomu; Rudd, Christopher E.; Tedder, Thomas F.; Schlossman, Stuart F.; Morimoto, Chikao.

In: Cellular Immunology, Vol. 118, No. 1, 1989, p. 68-84.

Research output: Contribution to journalArticle

Takeuchi, Tsutomu ; Rudd, Christopher E. ; Tedder, Thomas F. ; Schlossman, Stuart F. ; Morimoto, Chikao. / Amplification of suppressor inducer pathway with monoclonal antibody, anti-2H4, identifying a novel epitope of the common leukocyte antigen/T200 antigen. In: Cellular Immunology. 1989 ; Vol. 118, No. 1. pp. 68-84.
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