An Acidic Polysaccharide Having Immunological Activities from the Rhizome of Cnidium officinale

Masashi Tomoda, Naoko Ohara, Ryoko Gonda, Noriko Shimizu, Katsutoshi Takada, Yasuyo Satoh, Sayuri Shirai

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15 Citations (Scopus)

Abstract

An acidic polysaccharide, designated as cnidirhan AG, was isolated from the rhizomes of Cnidium officinale makino. It was homogeneous on electrophoresis and gel chromatography, and its molecular mass was estimated to be 5.1 x 104. It showed pronounced reticuloendothelial system-potentiating activity in a carbon clearance test, and had a remarkable effect on both anti-complementary and alkaline phosphatase-inducing activities. It is composed of L-arabinose: D-galactose: D-glucuronic acid in the molar ratio of 2:6:1, in addition to small amounts of O-acetyl groups. Methylation analysis, carbon-13 nuclear magnetic resonance, controlled Smith degradation and limited acid hydrolysis indicated that the core structural features of cnidirhan AG include a backbone chain composed of j?-l,3-linked D-galactose residues. Some of the galactose units in the backbone carry /?-D-galactosyl side chains at position 6. Both a-L-arabinosyl arabinose side chains and terminal /J-D-glucuronic acid residues are linked to the core galactan units.

Original languageEnglish
Pages (from-to)3025-3029
Number of pages5
JournalChemical and Pharmaceutical Bulletin
Volume40
Issue number11
DOIs
Publication statusPublished - 1992 Jan 1
Externally publishedYes

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Keywords

  • Cnidium officinale
  • Smith degradation
  • acidic arabinogalactan
  • alkaline phosphatase-inducing activity
  • anti-complementary activity
  • cnidirhan AG
  • immunological activity
  • polysaccharide structure
  • reticuloendothelial system
  • rhizome

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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