An activity stain for dihydroxy-acid dehydratase

Che Fu Kuo, Tadahiko Mashino, Irwin Fridovich

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An activity stain has been devised for the dihydroxy-acid dehydratase. When applied to polyacrylamide gel electropherograms of crude soluble extracts of Escherichia coli, it detected a single electromorph. The intensity of staining increased with the amount of extract protein applied to the gel. Activity staining demonstrated that (a) anaerobically grown cells contain more extractable dehydratase activity than do aerobically grown cells; (b) exposure of E. coli to 4.2 atm 02 caused virtually complete loss of activity; (c) exposure of cells to paraquat or plumbagin in the presence of dioxygen, but not in its absence, caused a massive loss of activity. These data illustrate the utility of this activity stain and demonstrate that the dehydratase is inactivated by O2- generated within cells.

Original languageEnglish
Pages (from-to)526-530
Number of pages5
JournalAnalytical Biochemistry
Volume164
Issue number2
DOIs
Publication statusPublished - 1987 Aug 1
Externally publishedYes

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Hydro-Lyases
Coloring Agents
Escherichia coli
Acids
Paraquat
Staining and Labeling
Gels
Complex Mixtures
Oxygen
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

An activity stain for dihydroxy-acid dehydratase. / Kuo, Che Fu; Mashino, Tadahiko; Fridovich, Irwin.

In: Analytical Biochemistry, Vol. 164, No. 2, 01.08.1987, p. 526-530.

Research output: Contribution to journalArticle

Kuo, Che Fu ; Mashino, Tadahiko ; Fridovich, Irwin. / An activity stain for dihydroxy-acid dehydratase. In: Analytical Biochemistry. 1987 ; Vol. 164, No. 2. pp. 526-530.
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