An allelic non-histocompatibility antigen with wide tissue distribution as a marker for chimerism in pigs

Y. Fuchimoto, C. Huang, A. Shimizu, J. Seebach, S. Arn, David H. Sachs

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

It is frequently useful in studies of transplantation to have available an antibody to a cell surface antigen, which is not itself responsible for transplant rejection. In this paper we identify and describe such an antibody/antigen system in miniature swine. The monoclonal antibody, 1038H-10-9, was found to react to a pig allelic antigen (called PAA), found on a variety of pig cells and tissues, including peripheral blood mononuclear cells (PBMC), thymocytes, lymph node, bone marrow and skin. Analysis for recipient sensitization against PAA was performed by in vitro cell-mediated lympholysis (CML) assay, mixed lymphocyte reaction (MLR) assay, anti-body binding studies, and skin graft rejection patterns were examined. No evidence was found to indicate detection of PAA by any of these assays of alloreactivity. We therefore conclude that PAA, is an allelic swine cell surface antigen, with wide tissue distribution, and that it is not a histocompatibility antigen. It should provide a powerful tool for studies of transplantation biology in miniature swine, such as identification and quantification of chimerism following organ transplantation.

Original languageEnglish
Pages (from-to)43-52
Number of pages10
JournalTissue Antigens
Volume54
Issue number1
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Chimerism
Tissue Distribution
Miniature Swine
Swine
Graft Rejection
Surface Antigens
Antigens
Transplantation
Skin
Mixed Lymphocyte Culture Test
Histocompatibility Antigens
Antibodies
Organ Transplantation
Thymocytes
Blood Cells
Lymph Nodes
Bone Marrow
Monoclonal Antibodies

Keywords

  • Alleles
  • Antigens
  • Chimerism
  • Histocompatibility antigens
  • Miniature swine
  • Transplantation

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

An allelic non-histocompatibility antigen with wide tissue distribution as a marker for chimerism in pigs. / Fuchimoto, Y.; Huang, C.; Shimizu, A.; Seebach, J.; Arn, S.; Sachs, David H.

In: Tissue Antigens, Vol. 54, No. 1, 1999, p. 43-52.

Research output: Contribution to journalArticle

Fuchimoto, Y. ; Huang, C. ; Shimizu, A. ; Seebach, J. ; Arn, S. ; Sachs, David H. / An allelic non-histocompatibility antigen with wide tissue distribution as a marker for chimerism in pigs. In: Tissue Antigens. 1999 ; Vol. 54, No. 1. pp. 43-52.
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