Abstract
It is frequently useful in studies of transplantation to have available an antibody to a cell surface antigen, which is not itself responsible for transplant rejection. In this paper we identify and describe such an antibody/antigen system in miniature swine. The monoclonal antibody, 1038H-10-9, was found to react to a pig allelic antigen (called PAA), found on a variety of pig cells and tissues, including peripheral blood mononuclear cells (PBMC), thymocytes, lymph node, bone marrow and skin. Analysis for recipient sensitization against PAA was performed by in vitro cell-mediated lympholysis (CML) assay, mixed lymphocyte reaction (MLR) assay, anti-body binding studies, and skin graft rejection patterns were examined. No evidence was found to indicate detection of PAA by any of these assays of alloreactivity. We therefore conclude that PAA, is an allelic swine cell surface antigen, with wide tissue distribution, and that it is not a histocompatibility antigen. It should provide a powerful tool for studies of transplantation biology in miniature swine, such as identification and quantification of chimerism following organ transplantation.
| Original language | English |
|---|---|
| Pages (from-to) | 43-52 |
| Number of pages | 10 |
| Journal | Tissue Antigens |
| Volume | 54 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1999 |
| Externally published | Yes |
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Keywords
- Alleles
- Antigens
- Chimerism
- Histocompatibility antigens
- Miniature swine
- Transplantation
ASJC Scopus subject areas
- Immunology
- Cell Biology
Cite this
An allelic non-histocompatibility antigen with wide tissue distribution as a marker for chimerism in pigs. / Fuchimoto, Y.; Huang, C.; Shimizu, A.; Seebach, J.; Arn, S.; Sachs, David H.
In: Tissue Antigens, Vol. 54, No. 1, 1999, p. 43-52.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An allelic non-histocompatibility antigen with wide tissue distribution as a marker for chimerism in pigs
AU - Fuchimoto, Y.
AU - Huang, C.
AU - Shimizu, A.
AU - Seebach, J.
AU - Arn, S.
AU - Sachs, David H.
PY - 1999
Y1 - 1999
N2 - It is frequently useful in studies of transplantation to have available an antibody to a cell surface antigen, which is not itself responsible for transplant rejection. In this paper we identify and describe such an antibody/antigen system in miniature swine. The monoclonal antibody, 1038H-10-9, was found to react to a pig allelic antigen (called PAA), found on a variety of pig cells and tissues, including peripheral blood mononuclear cells (PBMC), thymocytes, lymph node, bone marrow and skin. Analysis for recipient sensitization against PAA was performed by in vitro cell-mediated lympholysis (CML) assay, mixed lymphocyte reaction (MLR) assay, anti-body binding studies, and skin graft rejection patterns were examined. No evidence was found to indicate detection of PAA by any of these assays of alloreactivity. We therefore conclude that PAA, is an allelic swine cell surface antigen, with wide tissue distribution, and that it is not a histocompatibility antigen. It should provide a powerful tool for studies of transplantation biology in miniature swine, such as identification and quantification of chimerism following organ transplantation.
AB - It is frequently useful in studies of transplantation to have available an antibody to a cell surface antigen, which is not itself responsible for transplant rejection. In this paper we identify and describe such an antibody/antigen system in miniature swine. The monoclonal antibody, 1038H-10-9, was found to react to a pig allelic antigen (called PAA), found on a variety of pig cells and tissues, including peripheral blood mononuclear cells (PBMC), thymocytes, lymph node, bone marrow and skin. Analysis for recipient sensitization against PAA was performed by in vitro cell-mediated lympholysis (CML) assay, mixed lymphocyte reaction (MLR) assay, anti-body binding studies, and skin graft rejection patterns were examined. No evidence was found to indicate detection of PAA by any of these assays of alloreactivity. We therefore conclude that PAA, is an allelic swine cell surface antigen, with wide tissue distribution, and that it is not a histocompatibility antigen. It should provide a powerful tool for studies of transplantation biology in miniature swine, such as identification and quantification of chimerism following organ transplantation.
KW - Alleles
KW - Antigens
KW - Chimerism
KW - Histocompatibility antigens
KW - Miniature swine
KW - Transplantation
UR - http://www.scopus.com/inward/record.url?scp=0032796836&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032796836&partnerID=8YFLogxK
U2 - 10.1034/j.1399-0039.1999.540105.x
DO - 10.1034/j.1399-0039.1999.540105.x
M3 - Article
C2 - 10458322
AN - SCOPUS:0032796836
VL - 54
SP - 43
EP - 52
JO - HLA
JF - HLA
SN - 2059-2302
IS - 1
ER -