An Alu-linked repetitive sequence corresponding to 280 amino acids is expressed in a novel bovine protein, but not in its human homologue

Takahiro Nobukuni, Mariko Kobayashi, Akira Omori, Sachiyo Ichinose, Toshihiko Iwanaga, Ichiro Takahashi, Katsuyuki Hashimoto, Seisuke Hattori, Kozo Kaibuchi, Yoshihiko Miyata, Toru Masui, Shintaro Iwashita

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A novel protein harboring a 280-amino acid region from an Alu-linked repetitive sequence (bovine Alu-like dimer-driven family) was isolated from a bovine brain S-100 fraction using monoclonal antibodies against a rat GTPase- activating protein that shares the same epitope. The protein has an apparent molecular mass of 97 kDa (p97). Western blot analysis using extracts prepared from various tissues showed p97 to be predominantly detected in brain and moderately in liver and lung. From sequence analysis of the cDNA encoding p97, it was found that the 840-base pair sequence homologous to a part of the bovine Alu-like dimer-driven family, which has never been shown to be expressed, occurs in the middle of the protein coding region. The protein also contains a pair of intramolecular repeats composed of 40 highly hydrophilic amino acids at the C terminus. Human cDNA homologous to p97 was cloned, and its nucleotide sequence demonstrates that the 840-base pair repetitive sequence and one of the intramolecular repeats are missing. We named p97 bovine BCNT after Bucentaur. These results show that bovine BCNT is a unique molecule and suggest that an analysis of the relationship between bovine bent and its human homologue may help further the understanding of gene organization and evolution.

Original languageEnglish
Pages (from-to)2801-2807
Number of pages7
JournalJournal of Biological Chemistry
Volume272
Issue number5
DOIs
Publication statusPublished - 1997
Externally publishedYes

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Alu Elements
Nucleic Acid Repetitive Sequences
Amino Acids
Dimers
Brain
Proteins
Complementary DNA
GTPase-Activating Proteins
Base Pairing
Molecular mass
Liver
Rats
Epitopes
Nucleotides
Genes
Monoclonal Antibodies
Tissue
Sequence Homology
Open Reading Frames
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

An Alu-linked repetitive sequence corresponding to 280 amino acids is expressed in a novel bovine protein, but not in its human homologue. / Nobukuni, Takahiro; Kobayashi, Mariko; Omori, Akira; Ichinose, Sachiyo; Iwanaga, Toshihiko; Takahashi, Ichiro; Hashimoto, Katsuyuki; Hattori, Seisuke; Kaibuchi, Kozo; Miyata, Yoshihiko; Masui, Toru; Iwashita, Shintaro.

In: Journal of Biological Chemistry, Vol. 272, No. 5, 1997, p. 2801-2807.

Research output: Contribution to journalArticle

Nobukuni, T, Kobayashi, M, Omori, A, Ichinose, S, Iwanaga, T, Takahashi, I, Hashimoto, K, Hattori, S, Kaibuchi, K, Miyata, Y, Masui, T & Iwashita, S 1997, 'An Alu-linked repetitive sequence corresponding to 280 amino acids is expressed in a novel bovine protein, but not in its human homologue', Journal of Biological Chemistry, vol. 272, no. 5, pp. 2801-2807. https://doi.org/10.1074/jbc.272.5.2801
Nobukuni, Takahiro ; Kobayashi, Mariko ; Omori, Akira ; Ichinose, Sachiyo ; Iwanaga, Toshihiko ; Takahashi, Ichiro ; Hashimoto, Katsuyuki ; Hattori, Seisuke ; Kaibuchi, Kozo ; Miyata, Yoshihiko ; Masui, Toru ; Iwashita, Shintaro. / An Alu-linked repetitive sequence corresponding to 280 amino acids is expressed in a novel bovine protein, but not in its human homologue. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 5. pp. 2801-2807.
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