An angle-tunable microflap toward the observation of parasite invasion into host adherent cells

Tetsuhiko Teshima; Hiroaki Onoe; Hiroka Aonuma; Kaori Kuribayashi-Shigetomi; Hirotaka Kanuka; Shoji Takeuchi

An angle-tunable microflap toward the observation of parasite invasion into host adherent cells

Tetsuhiko Teshima, Hiroaki Onoe, Hiroka Aonuma, Kaori Kuribayashi-Shigetomi, Hirotaka Kanuka, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this paper, we demonstrate the angle-tunable microflap system for observing parasite invasion into adherent host cells. This system enables us to control the inclination angle of cell-culturing microflaps by applying external magnetic fields. A fabricated microflap consists of two materials, parylene and permalloy, and adherent host cells are patterned on the microflaps. We successfully kept adherent cells on the microflaps inclined by an external magnetic field, and observed boundary of cell membrane. Thus, we clearly observed how parasites invaded through cell membrane from multiple angles, and judged completed invasion. This method is widely applicable to the analysis of interaction between infectious microbes and host cells.

Original language	English
Title of host publication	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Publisher	Chemical and Biological Microsystems Society
Pages	103-105
Number of pages	3
ISBN (Print)	9780979806452
Publication status	Published - 2012 Jan 1
Externally published	Yes
Event	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 2012 Oct 28 → 2012 Nov 1

Publication series

Name	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country	Japan
City	Okinawa
Period	12/10/28 → 12/11/1

Keywords

Microflap
Parasite invasion
Parylene

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

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Cite this

Teshima, T., Onoe, H., Aonuma, H., Kuribayashi-Shigetomi, K., Kanuka, H., & Takeuchi, S. (2012). An angle-tunable microflap toward the observation of parasite invasion into host adherent cells. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 103-105). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.

An angle-tunable microflap toward the observation of parasite invasion into host adherent cells. / Teshima, Tetsuhiko; Onoe, Hiroaki; Aonuma, Hiroka; Kuribayashi-Shigetomi, Kaori; Kanuka, Hirotaka; Takeuchi, Shoji.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 103-105 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Teshima, T, Onoe, H, Aonuma, H, Kuribayashi-Shigetomi, K, Kanuka, H & Takeuchi, S 2012, An angle-tunable microflap toward the observation of parasite invasion into host adherent cells. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Chemical and Biological Microsystems Society, pp. 103-105, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12/10/28.

Teshima T, Onoe H, Aonuma H, Kuribayashi-Shigetomi K, Kanuka H, Takeuchi S. An angle-tunable microflap toward the observation of parasite invasion into host adherent cells. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 103-105. (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Teshima, Tetsuhiko ; Onoe, Hiroaki ; Aonuma, Hiroka ; Kuribayashi-Shigetomi, Kaori ; Kanuka, Hirotaka ; Takeuchi, Shoji. / An angle-tunable microflap toward the observation of parasite invasion into host adherent cells. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 103-105 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

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AU - Kanuka, Hirotaka

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