TY - GEN
T1 - An angle-tunable microflap toward the observation of parasite invasion into host adherent cells
AU - Teshima, Tetsuhiko
AU - Onoe, Hiroaki
AU - Aonuma, Hiroka
AU - Kuribayashi-Shigetomi, Kaori
AU - Kanuka, Hirotaka
AU - Takeuchi, Shoji
PY - 2012/1/1
Y1 - 2012/1/1
N2 - In this paper, we demonstrate the angle-tunable microflap system for observing parasite invasion into adherent host cells. This system enables us to control the inclination angle of cell-culturing microflaps by applying external magnetic fields. A fabricated microflap consists of two materials, parylene and permalloy, and adherent host cells are patterned on the microflaps. We successfully kept adherent cells on the microflaps inclined by an external magnetic field, and observed boundary of cell membrane. Thus, we clearly observed how parasites invaded through cell membrane from multiple angles, and judged completed invasion. This method is widely applicable to the analysis of interaction between infectious microbes and host cells.
AB - In this paper, we demonstrate the angle-tunable microflap system for observing parasite invasion into adherent host cells. This system enables us to control the inclination angle of cell-culturing microflaps by applying external magnetic fields. A fabricated microflap consists of two materials, parylene and permalloy, and adherent host cells are patterned on the microflaps. We successfully kept adherent cells on the microflaps inclined by an external magnetic field, and observed boundary of cell membrane. Thus, we clearly observed how parasites invaded through cell membrane from multiple angles, and judged completed invasion. This method is widely applicable to the analysis of interaction between infectious microbes and host cells.
KW - Microflap
KW - Parasite invasion
KW - Parylene
UR - http://www.scopus.com/inward/record.url?scp=84901804316&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901804316&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901804316
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 103
EP - 105
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -