An angle-tunable microflap toward the observation of parasite invasion into host adherent cells

Tetsuhiko Teshima, Hiroaki Onoe, Hiroka Aonuma, Kaori Kuribayashi-Shigetomi, Hirotaka Kanuka, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we demonstrate the angle-tunable microflap system for observing parasite invasion into adherent host cells. This system enables us to control the inclination angle of cell-culturing microflaps by applying external magnetic fields. A fabricated microflap consists of two materials, parylene and permalloy, and adherent host cells are patterned on the microflaps. We successfully kept adherent cells on the microflaps inclined by an external magnetic field, and observed boundary of cell membrane. Thus, we clearly observed how parasites invaded through cell membrane from multiple angles, and judged completed invasion. This method is widely applicable to the analysis of interaction between infectious microbes and host cells.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages103-105
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Keywords

  • Microflap
  • Parasite invasion
  • Parylene

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Teshima, T., Onoe, H., Aonuma, H., Kuribayashi-Shigetomi, K., Kanuka, H., & Takeuchi, S. (2012). An angle-tunable microflap toward the observation of parasite invasion into host adherent cells. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 103-105). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.