Abstract
In this paper, we demonstrate the angle-tunable microflap system for observing parasite invasion into adherent host cells. This system enables us to control the inclination angle of cell-culturing microflaps by applying external magnetic fields. A fabricated microflap consists of two materials, parylene and permalloy, and adherent host cells are patterned on the microflaps. We successfully kept adherent cells on the microflaps inclined by an external magnetic field, and observed boundary of cell membrane. Thus, we clearly observed how parasites invaded through cell membrane from multiple angles, and judged completed invasion. This method is widely applicable to the analysis of interaction between infectious microbes and host cells.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 103-105 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806452 |
| Publication status | Published - 2012 Jan 1 |
| Externally published | Yes |
| Event | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 2012 Oct 28 → 2012 Nov 1 |
Publication series
| Name | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|
Other
| Other | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|---|
| Country | Japan |
| City | Okinawa |
| Period | 12/10/28 → 12/11/1 |
Keywords
- Microflap
- Parasite invasion
- Parylene
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
Fingerprint Dive into the research topics of 'An angle-tunable microflap toward the observation of parasite invasion into host adherent cells'. Together they form a unique fingerprint.
Cite this
Teshima, T., Onoe, H., Aonuma, H., Kuribayashi-Shigetomi, K., Kanuka, H., & Takeuchi, S. (2012). An angle-tunable microflap toward the observation of parasite invasion into host adherent cells. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 103-105). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.