Abstract
In this paper, we demonstrate the angle-tunable microflap system for observing parasite invasion into adherent host cells. This system enables us to control the inclination angle of cell-culturing microflaps by applying external magnetic fields. A fabricated microflap consists of two materials, parylene and permalloy, and adherent host cells are patterned on the microflaps. We successfully kept adherent cells on the microflaps inclined by an external magnetic field, and observed boundary of cell membrane. Thus, we clearly observed how parasites invaded through cell membrane from multiple angles, and judged completed invasion. This method is widely applicable to the analysis of interaction between infectious microbes and host cells.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 103-105 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806452 |
| Publication status | Published - 2012 |
| Externally published | Yes |
| Event | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 2012 Oct 28 → 2012 Nov 1 |
Other
| Other | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|---|
| Country | Japan |
| City | Okinawa |
| Period | 12/10/28 → 12/11/1 |
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Keywords
- Microflap
- Parasite invasion
- Parylene
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
Cite this
An angle-tunable microflap toward the observation of parasite invasion into host adherent cells. / Teshima, Tetsuhiko; Onoe, Hiroaki; Aonuma, Hiroka; Kuribayashi-Shigetomi, Kaori; Kanuka, Hirotaka; Takeuchi, Shoji.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 103-105.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - An angle-tunable microflap toward the observation of parasite invasion into host adherent cells
AU - Teshima, Tetsuhiko
AU - Onoe, Hiroaki
AU - Aonuma, Hiroka
AU - Kuribayashi-Shigetomi, Kaori
AU - Kanuka, Hirotaka
AU - Takeuchi, Shoji
PY - 2012
Y1 - 2012
N2 - In this paper, we demonstrate the angle-tunable microflap system for observing parasite invasion into adherent host cells. This system enables us to control the inclination angle of cell-culturing microflaps by applying external magnetic fields. A fabricated microflap consists of two materials, parylene and permalloy, and adherent host cells are patterned on the microflaps. We successfully kept adherent cells on the microflaps inclined by an external magnetic field, and observed boundary of cell membrane. Thus, we clearly observed how parasites invaded through cell membrane from multiple angles, and judged completed invasion. This method is widely applicable to the analysis of interaction between infectious microbes and host cells.
AB - In this paper, we demonstrate the angle-tunable microflap system for observing parasite invasion into adherent host cells. This system enables us to control the inclination angle of cell-culturing microflaps by applying external magnetic fields. A fabricated microflap consists of two materials, parylene and permalloy, and adherent host cells are patterned on the microflaps. We successfully kept adherent cells on the microflaps inclined by an external magnetic field, and observed boundary of cell membrane. Thus, we clearly observed how parasites invaded through cell membrane from multiple angles, and judged completed invasion. This method is widely applicable to the analysis of interaction between infectious microbes and host cells.
KW - Microflap
KW - Parasite invasion
KW - Parylene
UR - http://www.scopus.com/inward/record.url?scp=84901804316&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901804316&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901804316
SN - 9780979806452
SP - 103
EP - 105
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
ER -