An anti-inflammatory role for carbon monoxide and heme oxygenase-1 in chronic Th2-mediated murine colitis

Shehzad Z. Sheikh, Refaat A. Hegazi, Taku Kobayashi, Joseph C. Onyiah, Steven M. Russo, Katsuyoshi Matsuoka, Antonia R. Sepulveda, Fengling Li, Leo E. Otterbein, Scott E. Plevy

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Abstract

Cigarette smoking is a significant environmental factor in the human inflammatory bowel diseases, remarkably, conferring protection in ulcerative colitis. We previously demonstrated that a prominent component of cigarette smoke, CO, suppresses Th17-mediated experimental colitis in IL-10-/- mice through a heme oxygenase (HO)-1-dependent pathway. In this study, homeostatic and therapeutic effects of CO and HO-1 were determined in chronic colonic inflammation in TCR-α-deficient (-/-) mice, in which colitis is mediated by Th2 cytokines, similar to the cytokine milieu described in human ulcerative colitis. TCRα-/- mice exposed to CO or treated with the pharmacologic HO-1 inducer cobalt protoporphyrin demonstrated amelioration of active colitis. CO and cobalt protoporphyrin suppressed colonic IL-1β, TNF, and IL-4 production, whereas IL-10 protein secretion was increased. CO induced IL-10 expression in macrophages and in vivo through an HO-1-dependent pathway. Bacterial products regulate HO-1 expression in macrophages through MyD88- and IL-10-dependent pathways. CO exposure and pharmacologic HO-1 induction in vivo resulted in increased expression of HO-1 and IL-10 in CD11b+ lamina propria mononuclear cells. Moreover, induction of the IL-10 family member IL-22 was demonstrated in CD11b- lamina propria mononuclear cells. In conclusion, CO and HO-1 induction ameliorated active colitis in TCRβ-/- mice, and therapeutic effects correlated with induction of IL-10. This study provides further evidence that HO-1 mediates an important homeostatic pathway with pleiotropic anti-inflammatory effects in different experimental models of colitis and that targeting HO-1, therefore, is a potential therapeutic strategy in human inflammatory bowel diseases.

Original languageEnglish
Pages (from-to)5506-5513
Number of pages8
JournalJournal of Immunology
Volume186
Issue number9
DOIs
Publication statusPublished - 2011 May 1

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Heme Oxygenase-1
Colitis
Carbon Monoxide
Anti-Inflammatory Agents
Interleukin-10
Therapeutic Uses
Ulcerative Colitis
Inflammatory Bowel Diseases
Mucous Membrane
Macrophages
Cytokines
Interleukin-1
Smoke
Tobacco Products
Interleukin-4
Theoretical Models
Smoking
Inflammation

ASJC Scopus subject areas

  • Immunology

Cite this

Sheikh, S. Z., Hegazi, R. A., Kobayashi, T., Onyiah, J. C., Russo, S. M., Matsuoka, K., ... Plevy, S. E. (2011). An anti-inflammatory role for carbon monoxide and heme oxygenase-1 in chronic Th2-mediated murine colitis. Journal of Immunology, 186(9), 5506-5513. https://doi.org/10.4049/jimmunol.1002433

An anti-inflammatory role for carbon monoxide and heme oxygenase-1 in chronic Th2-mediated murine colitis. / Sheikh, Shehzad Z.; Hegazi, Refaat A.; Kobayashi, Taku; Onyiah, Joseph C.; Russo, Steven M.; Matsuoka, Katsuyoshi; Sepulveda, Antonia R.; Li, Fengling; Otterbein, Leo E.; Plevy, Scott E.

In: Journal of Immunology, Vol. 186, No. 9, 01.05.2011, p. 5506-5513.

Research output: Contribution to journalArticle

Sheikh, SZ, Hegazi, RA, Kobayashi, T, Onyiah, JC, Russo, SM, Matsuoka, K, Sepulveda, AR, Li, F, Otterbein, LE & Plevy, SE 2011, 'An anti-inflammatory role for carbon monoxide and heme oxygenase-1 in chronic Th2-mediated murine colitis', Journal of Immunology, vol. 186, no. 9, pp. 5506-5513. https://doi.org/10.4049/jimmunol.1002433
Sheikh, Shehzad Z. ; Hegazi, Refaat A. ; Kobayashi, Taku ; Onyiah, Joseph C. ; Russo, Steven M. ; Matsuoka, Katsuyoshi ; Sepulveda, Antonia R. ; Li, Fengling ; Otterbein, Leo E. ; Plevy, Scott E. / An anti-inflammatory role for carbon monoxide and heme oxygenase-1 in chronic Th2-mediated murine colitis. In: Journal of Immunology. 2011 ; Vol. 186, No. 9. pp. 5506-5513.
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