An Entamoeba sp. strain isolated from rhesus monkey is virulent but genetically different from Entamoeba histolytica

Hiroshi Tachibana, Tetsuo Yanagi, Kishor Pandey, Xun Jia Cheng, Seiki Kobayashi, Jeevan B. Sherchand, Hiroji Kanbara

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

An Entamoeba sp. strain, P19-061405, was isolated from a rhesus monkey in Nepal and characterized genetically. The strain was initially identified as Entamoeba histolytica using PCR amplification of peroxiredoxin genes. However, sequence analysis of the 18S rRNA gene showed a 0.8% difference when compared to the reference E. histolytica HM-1:IMSS human strain. Differences were also observed in the 5.8S rRNA gene and the internal transcribed spacer (ITS) regions 1 and 2, and analysis of the serine-rich protein gene from the monkey strain showed unique codon usages compared to E. histolytica isolated from humans. The amino acid sequences of two hexokinases and two glucose phosphate isomerases also differed from those of E. histolytica. Isoenzyme analyses of these enzymes in the monkey strain showed different electrophoretic mobility patterns compared with E. histolytica isolates. Analysis of peroxiredoxin genes indicated the presence of at least seven different types of protein, none of which were identical to proteins in E. histolytica. When the trophozoites from the monkey strain were inoculated into the livers of hamsters, formation of amebic abscesses was observed 7 days after the injection. These results demonstrate that the strain is genetically different from E. histolytica and is virulent. Revival of the name Entamoeba nuttalli is proposed for the organism.

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalMolecular and Biochemical Parasitology
Volume153
Issue number2
DOIs
Publication statusPublished - 2007 Jun

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Entamoeba
Entamoeba histolytica
Macaca mulatta
Haplorhini
Peroxiredoxins
rRNA Genes
xylose isomerase
Amebiasis
Trophozoites
Nepal
Proteins
Hexokinase
Gene Amplification
Codon
Cricetinae
Serine
Isoenzymes
Names
Sequence Analysis
Amino Acid Sequence

Keywords

  • Entamoeba histolytica
  • Entamoeba nuttalli
  • Rhesus monkey
  • rRNA gene
  • Virulency

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

An Entamoeba sp. strain isolated from rhesus monkey is virulent but genetically different from Entamoeba histolytica. / Tachibana, Hiroshi; Yanagi, Tetsuo; Pandey, Kishor; Cheng, Xun Jia; Kobayashi, Seiki; Sherchand, Jeevan B.; Kanbara, Hiroji.

In: Molecular and Biochemical Parasitology, Vol. 153, No. 2, 06.2007, p. 107-114.

Research output: Contribution to journalArticle

Tachibana, Hiroshi ; Yanagi, Tetsuo ; Pandey, Kishor ; Cheng, Xun Jia ; Kobayashi, Seiki ; Sherchand, Jeevan B. ; Kanbara, Hiroji. / An Entamoeba sp. strain isolated from rhesus monkey is virulent but genetically different from Entamoeba histolytica. In: Molecular and Biochemical Parasitology. 2007 ; Vol. 153, No. 2. pp. 107-114.
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