An evaluation of semen processing methods for eliminating HIV-1

Hideji Hanabusa, Naoaki Kuji, Shingo Kato, Hisamichi Tagami, Satoru Kaneko, Hiroaki Tanaka, Yasunori Yoshimura

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Objective: Whether artificial insemination can provide adequate protection for discordant couples where the man is HIV-1 positive and the woman is HIV-1 negative is uncertain because of the paucity of HIV-1 elimination data assessing current sperm-washing techniques. We evaluated how effectively these techniques eliminate HIV-1 RNA and proviral DNA from semen. Methods: Spermatozoa were separated from semen samples from HIV-1-positive patients with haemophilia by discontinuous Percoll gradient centrifugation and the 'swim-up' method. The HIV-1 RNA and proviral DNA were measured by a highly sensitive PCR. In another test 5 x 106 copies of HIV-1 RNA (LAI strain) were added to semen from healthy donors and then assessed after single and combined procedures. Results: Swim-up processing after Percoll gradient centrifugation reduced HIV-1 RNA and HIV-1 proviral DNA in semen to undetectable levels in the original specimen. Although discontinuous and continuous Percoll gradient centrifugation respectively reduced HIV-1 RNA added to seminal plasma specimens from healthy donors to less than < 1 copy from 105 and about 1 copy per 103 pre-separation copies, the discontinuous method left detectable HIV-1 RNA and proviral DNA in one out of 12 samples from patients with HIV-1 infection (8%). HIV-1 RNA and proviral DNA were decreased to undetectable levels after adding the swim-up procedure. Conclusions: Swim-up separation following Percoll gradient centrifugation should offer adequate protection for HIV-1-discordant couples. (C) 2000 Lippincot Williams and Wilkins.

Original languageEnglish
Pages (from-to)1611-1616
Number of pages6
JournalAIDS
Volume14
Issue number11
DOIs
Publication statusPublished - 2000

Fingerprint

Semen
HIV-1
RNA
Centrifugation
DNA
Spermatozoa
Tissue Donors
Artificial Insemination
Hemophilia A
HIV Infections

Keywords

  • DNA
  • HIV
  • Percoll centrifugation
  • RNA
  • Semen
  • Spermatozoa
  • Swim-up
  • Transmission

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Hanabusa, H., Kuji, N., Kato, S., Tagami, H., Kaneko, S., Tanaka, H., & Yoshimura, Y. (2000). An evaluation of semen processing methods for eliminating HIV-1. AIDS, 14(11), 1611-1616. https://doi.org/10.1097/00002030-200007280-00017

An evaluation of semen processing methods for eliminating HIV-1. / Hanabusa, Hideji; Kuji, Naoaki; Kato, Shingo; Tagami, Hisamichi; Kaneko, Satoru; Tanaka, Hiroaki; Yoshimura, Yasunori.

In: AIDS, Vol. 14, No. 11, 2000, p. 1611-1616.

Research output: Contribution to journalArticle

Hanabusa, H, Kuji, N, Kato, S, Tagami, H, Kaneko, S, Tanaka, H & Yoshimura, Y 2000, 'An evaluation of semen processing methods for eliminating HIV-1', AIDS, vol. 14, no. 11, pp. 1611-1616. https://doi.org/10.1097/00002030-200007280-00017
Hanabusa H, Kuji N, Kato S, Tagami H, Kaneko S, Tanaka H et al. An evaluation of semen processing methods for eliminating HIV-1. AIDS. 2000;14(11):1611-1616. https://doi.org/10.1097/00002030-200007280-00017
Hanabusa, Hideji ; Kuji, Naoaki ; Kato, Shingo ; Tagami, Hisamichi ; Kaneko, Satoru ; Tanaka, Hiroaki ; Yoshimura, Yasunori. / An evaluation of semen processing methods for eliminating HIV-1. In: AIDS. 2000 ; Vol. 14, No. 11. pp. 1611-1616.
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