An experimental study on costal osteochondral graft

Kazuki Satou, O. J. Moy, C. A. Peimer, T. Nakamura, C. Howard, S. H. Ko, T. C. Lee, Y. Nishiwaki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective: To investigate usefulness of osteochondral grafting from the costo-osteochondral junction as a repair technique for articular cartilage defects histologic and biochemical analysis of grafted cartilage in rabbit knees was evaluated up to 48. weeks after transplantation. Methods: Twenty New Zealand White rabbits were used. A costal osteochondral plug was harvested from a middle rib. After trimming, it was transplanted into a cylindrical osteochondral 2.5. mm diameter and 5. mm deep defect created in the knee. The animals were sacrificed at 6, 12, 24, and 48. weeks after transplantation. Defect sites were inspected macroscopically, and then by light microscopy. Samples were evaluated for cell viability using a fluorescent. in situ double-staining protocol with confocal laser microscopic analysis. Samples were also processed to assess type I and II collagen and aggrecan mRNA expression using reverse transcription-polymerase chain reaction (RT-PCR). Results: Histologically, bone union was achieved in all plugs. Confocal microscopic analysis revealed chondrocyte viability in the 48-week grafts; the distribution of chondrocytes was similar to surrounding articular cartilage. The expression of type II collagen and aggrecan mRNA in the grafted cartilage was consistent with normal articular cartilage and normal costal cartilage. These results were observed over 6-48. weeks. Conclusions: Our study revealed that chondrocytes in the grafted cartilage were viable at least up to 48. weeks and that mRNA expression of type II collagen and aggrecan was also similar to that of normal articular cartilage. These results suggest that costal osteochondral grafting can be a useful alternative in the treatment of osteochondral defects.

Original languageEnglish
Pages (from-to)172-183
Number of pages12
JournalOsteoarthritis and Cartilage
Volume20
Issue number2
DOIs
Publication statusPublished - 2012 Feb

Fingerprint

Cartilage
Articular Cartilage
Aggrecans
Grafts
Collagen Type II
Chondrocytes
Transplants
Messenger RNA
Knee
Transplantation
Collagen
Rabbits
Defects
Ribs
Collagen Type I
Reverse Transcription
Microscopy
Cell Survival
Lasers
Staining and Labeling

Keywords

  • Articular cartilage
  • Costal osteochondral graft
  • Osteochondral graft
  • Rabbit
  • Rib

ASJC Scopus subject areas

  • Biomedical Engineering
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

Satou, K., Moy, O. J., Peimer, C. A., Nakamura, T., Howard, C., Ko, S. H., ... Nishiwaki, Y. (2012). An experimental study on costal osteochondral graft. Osteoarthritis and Cartilage, 20(2), 172-183. https://doi.org/10.1016/j.joca.2011.12.001

An experimental study on costal osteochondral graft. / Satou, Kazuki; Moy, O. J.; Peimer, C. A.; Nakamura, T.; Howard, C.; Ko, S. H.; Lee, T. C.; Nishiwaki, Y.

In: Osteoarthritis and Cartilage, Vol. 20, No. 2, 02.2012, p. 172-183.

Research output: Contribution to journalArticle

Satou, K, Moy, OJ, Peimer, CA, Nakamura, T, Howard, C, Ko, SH, Lee, TC & Nishiwaki, Y 2012, 'An experimental study on costal osteochondral graft', Osteoarthritis and Cartilage, vol. 20, no. 2, pp. 172-183. https://doi.org/10.1016/j.joca.2011.12.001
Satou, Kazuki ; Moy, O. J. ; Peimer, C. A. ; Nakamura, T. ; Howard, C. ; Ko, S. H. ; Lee, T. C. ; Nishiwaki, Y. / An experimental study on costal osteochondral graft. In: Osteoarthritis and Cartilage. 2012 ; Vol. 20, No. 2. pp. 172-183.
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