An extreme thermophile, Thermus thermophilus, is a polyploid bacterium

Naoto Ohtani, Masaru Tomita, Mitsuhiro Itaya

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

An extremely thermophilic bacterium, Thermus thermophilus HB8, is one of the model organisms for systems biology. Its genome consists of a chromosome (1.85 Mb), a megaplasmid (0.26 Mb) designated pTT27, and a plasmid (9.3 kb) designated pTT8, and the complete sequence is available. We show here that T. thermophilus is a polyploid organism, harboring multiple genomic copies in a cell. In the case of the HB8 strain, the copy number of the chromosome was estimated to be four or five, and the copy number of the pTT27 megaplasmid seemed to be equal to that of the chromosome. It has never been discussed whether T. thermophilus is haploid or polyploid. However, the finding that it is polyploid is not surprising, as Deinococcus radiodurans, an extremely radioresistant bacterium closely related to Thermus, is well known to be a polyploid organism. As is the case for D. radiodurans in the radiation environment, the polyploidy of T. thermophilus might allow for genomic DNA protection, maintenance, and repair at elevated growth temperatures. Polyploidy often complicates the recognition of an essential gene in T. thermophilus as a model organism for systems biology.

Original languageEnglish
Pages (from-to)5499-5505
Number of pages7
JournalJournal of Bacteriology
Volume192
Issue number20
DOIs
Publication statusPublished - 2010 Oct 1

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Thermus thermophilus
Polyploidy
Bacteria
Deinococcus
Systems Biology
Chromosomes
Thermus
Haploidy
Essential Genes
Plasmids
Maintenance
Genome
Radiation
Temperature
DNA
Growth

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Medicine(all)

Cite this

An extreme thermophile, Thermus thermophilus, is a polyploid bacterium. / Ohtani, Naoto; Tomita, Masaru; Itaya, Mitsuhiro.

In: Journal of Bacteriology, Vol. 192, No. 20, 01.10.2010, p. 5499-5505.

Research output: Contribution to journalArticle

Ohtani, Naoto ; Tomita, Masaru ; Itaya, Mitsuhiro. / An extreme thermophile, Thermus thermophilus, is a polyploid bacterium. In: Journal of Bacteriology. 2010 ; Vol. 192, No. 20. pp. 5499-5505.
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