An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid

Li Linxiang, Yoshihiro Abe, Yukiko Nagasawa, Risa Kudo, Noriko Usui, Kazuhiro Imai, Tadahiko Mashino, Masataka Mochizuki, Naoki Miyata

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Abstract

An HPLC assay for hydroxyl radicals is described. The hydroxyl radical was trapped by terephthalic acid (non-fluorescent), and 2-hydroxyl terephthalic acid (fluorescent) was quantitated by HPLC-fluorescence detection. At a terephthalic acid concentration of 4.25 μmol/L, the hydroxyl radical formed in the Fenton reaction was successfully assayed in the concentration range of hydrogen peroxide of 2.5-50 μmol/L, where the concentration of Fe(II) was 50 μmol/L. The fluorescence of 2-hydroxy terephthalate was stable at 24 h, and its detection limit by this method was 5 nmol/L (100 fmol).

Original languageEnglish
Pages (from-to)470-474
Number of pages5
JournalBiomedical Chromatography
Volume18
Issue number7
DOIs
Publication statusPublished - 2004 Sep

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Keywords

  • 2-Hydroxyterephthalic acid
  • Fenton reaction
  • Fluorescence detection
  • HPLC
  • Hydroxyl radical
  • Terephthalic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

Cite this

Linxiang, L., Abe, Y., Nagasawa, Y., Kudo, R., Usui, N., Imai, K., Mashino, T., Mochizuki, M., & Miyata, N. (2004). An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid. Biomedical Chromatography, 18(7), 470-474. https://doi.org/10.1002/bmc.339