An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid

Li Linxiang, Yoshihiro Abe, Yukiko Nagasawa, Risa Kudo, Noriko Usui, Kazuhiro Imai, Tadahiko Mashino, Masataka Mochizuki, Naoki Miyata

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

An HPLC assay for hydroxyl radicals is described. The hydroxyl radical was trapped by terephthalic acid (non-fluorescent), and 2-hydroxyl terephthalic acid (fluorescent) was quantitated by HPLC-fluorescence detection. At a terephthalic acid concentration of 4.25 μmol/L, the hydroxyl radical formed in the Fenton reaction was successfully assayed in the concentration range of hydrogen peroxide of 2.5-50 μmol/L, where the concentration of Fe(II) was 50 μmol/L. The fluorescence of 2-hydroxy terephthalate was stable at 24 h, and its detection limit by this method was 5 nmol/L (100 fmol).

Original languageEnglish
Pages (from-to)470-474
Number of pages5
JournalBiomedical Chromatography
Volume18
Issue number7
DOIs
Publication statusPublished - 2004 Sep

Fingerprint

Hydroxylation
Hydroxyl Radical
Assays
High Pressure Liquid Chromatography
Fluorescence
Hydrogen Peroxide
Limit of Detection
terephthalic acid

Keywords

  • 2-Hydroxyterephthalic acid
  • Fenton reaction
  • Fluorescence detection
  • HPLC
  • Hydroxyl radical
  • Terephthalic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

Cite this

Linxiang, L., Abe, Y., Nagasawa, Y., Kudo, R., Usui, N., Imai, K., ... Miyata, N. (2004). An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid. Biomedical Chromatography, 18(7), 470-474. https://doi.org/10.1002/bmc.339

An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid. / Linxiang, Li; Abe, Yoshihiro; Nagasawa, Yukiko; Kudo, Risa; Usui, Noriko; Imai, Kazuhiro; Mashino, Tadahiko; Mochizuki, Masataka; Miyata, Naoki.

In: Biomedical Chromatography, Vol. 18, No. 7, 09.2004, p. 470-474.

Research output: Contribution to journalArticle

Linxiang, L, Abe, Y, Nagasawa, Y, Kudo, R, Usui, N, Imai, K, Mashino, T, Mochizuki, M & Miyata, N 2004, 'An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid', Biomedical Chromatography, vol. 18, no. 7, pp. 470-474. https://doi.org/10.1002/bmc.339
Linxiang, Li ; Abe, Yoshihiro ; Nagasawa, Yukiko ; Kudo, Risa ; Usui, Noriko ; Imai, Kazuhiro ; Mashino, Tadahiko ; Mochizuki, Masataka ; Miyata, Naoki. / An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid. In: Biomedical Chromatography. 2004 ; Vol. 18, No. 7. pp. 470-474.
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