An H+-Coupled Multidrug Efflux Pump, PmpM, a Member of the MATE Family of Transporters, from Pseudomonas aeruginosa

Gui Xin He, Teruo Kuroda, Takehiko Mima, Yuji Morita, Tohru Mizushima, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

We cloned the gene PA1361 (we designated the gene pmpM), which seemed to encode a multidrug efflux pump belonging to the MATE family, of Pseudomonas aeruginosa by the PCR method using the drug-hypersensitive Escherichia coli KAM32 strain as a host. Cells of E. coli possessing the pmpM gene showed elevated resistance to several antimicrobial agents. We observed energy-dependent efflux of ethidium from cells possessing the pmpM gene. We found that PmpM is an H+-drug antiporter, and this finding is the first reported case of an H+-coupled efflux pump in the MATE family. Disruption and reintroduction of the pmpM gene in P. aeruginosa revealed that PmpM is functional and that benzalkonium chloride, fluoroquinolones, ethidium bromide, acriflavine, and tetraphenylphosphonium chloride are substrates for PmpM in this microorganism.

Original languageEnglish
Pages (from-to)262-265
Number of pages4
JournalJournal of Bacteriology
Volume186
Issue number1
DOIs
Publication statusPublished - 2004 Jan
Externally publishedYes

Fingerprint

Pseudomonas aeruginosa
Ethidium
3,6-diamino-10-methylacridinium
Genes
Escherichia coli
Benzalkonium Compounds
Antiporters
Fluoroquinolones
Anti-Infective Agents
Pharmaceutical Preparations
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

An H+-Coupled Multidrug Efflux Pump, PmpM, a Member of the MATE Family of Transporters, from Pseudomonas aeruginosa. / He, Gui Xin; Kuroda, Teruo; Mima, Takehiko; Morita, Yuji; Mizushima, Tohru; Tsuchiya, Tomofusa.

In: Journal of Bacteriology, Vol. 186, No. 1, 01.2004, p. 262-265.

Research output: Contribution to journalArticle

He, Gui Xin ; Kuroda, Teruo ; Mima, Takehiko ; Morita, Yuji ; Mizushima, Tohru ; Tsuchiya, Tomofusa. / An H+-Coupled Multidrug Efflux Pump, PmpM, a Member of the MATE Family of Transporters, from Pseudomonas aeruginosa. In: Journal of Bacteriology. 2004 ; Vol. 186, No. 1. pp. 262-265.
@article{c0a92a35fdde43938b5e6082bc8b10d2,
title = "An H+-Coupled Multidrug Efflux Pump, PmpM, a Member of the MATE Family of Transporters, from Pseudomonas aeruginosa",
abstract = "We cloned the gene PA1361 (we designated the gene pmpM), which seemed to encode a multidrug efflux pump belonging to the MATE family, of Pseudomonas aeruginosa by the PCR method using the drug-hypersensitive Escherichia coli KAM32 strain as a host. Cells of E. coli possessing the pmpM gene showed elevated resistance to several antimicrobial agents. We observed energy-dependent efflux of ethidium from cells possessing the pmpM gene. We found that PmpM is an H+-drug antiporter, and this finding is the first reported case of an H+-coupled efflux pump in the MATE family. Disruption and reintroduction of the pmpM gene in P. aeruginosa revealed that PmpM is functional and that benzalkonium chloride, fluoroquinolones, ethidium bromide, acriflavine, and tetraphenylphosphonium chloride are substrates for PmpM in this microorganism.",
author = "He, {Gui Xin} and Teruo Kuroda and Takehiko Mima and Yuji Morita and Tohru Mizushima and Tomofusa Tsuchiya",
year = "2004",
month = "1",
doi = "10.1128/JB.186.1.262-265.2004",
language = "English",
volume = "186",
pages = "262--265",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - An H+-Coupled Multidrug Efflux Pump, PmpM, a Member of the MATE Family of Transporters, from Pseudomonas aeruginosa

AU - He, Gui Xin

AU - Kuroda, Teruo

AU - Mima, Takehiko

AU - Morita, Yuji

AU - Mizushima, Tohru

AU - Tsuchiya, Tomofusa

PY - 2004/1

Y1 - 2004/1

N2 - We cloned the gene PA1361 (we designated the gene pmpM), which seemed to encode a multidrug efflux pump belonging to the MATE family, of Pseudomonas aeruginosa by the PCR method using the drug-hypersensitive Escherichia coli KAM32 strain as a host. Cells of E. coli possessing the pmpM gene showed elevated resistance to several antimicrobial agents. We observed energy-dependent efflux of ethidium from cells possessing the pmpM gene. We found that PmpM is an H+-drug antiporter, and this finding is the first reported case of an H+-coupled efflux pump in the MATE family. Disruption and reintroduction of the pmpM gene in P. aeruginosa revealed that PmpM is functional and that benzalkonium chloride, fluoroquinolones, ethidium bromide, acriflavine, and tetraphenylphosphonium chloride are substrates for PmpM in this microorganism.

AB - We cloned the gene PA1361 (we designated the gene pmpM), which seemed to encode a multidrug efflux pump belonging to the MATE family, of Pseudomonas aeruginosa by the PCR method using the drug-hypersensitive Escherichia coli KAM32 strain as a host. Cells of E. coli possessing the pmpM gene showed elevated resistance to several antimicrobial agents. We observed energy-dependent efflux of ethidium from cells possessing the pmpM gene. We found that PmpM is an H+-drug antiporter, and this finding is the first reported case of an H+-coupled efflux pump in the MATE family. Disruption and reintroduction of the pmpM gene in P. aeruginosa revealed that PmpM is functional and that benzalkonium chloride, fluoroquinolones, ethidium bromide, acriflavine, and tetraphenylphosphonium chloride are substrates for PmpM in this microorganism.

UR - http://www.scopus.com/inward/record.url?scp=0346991732&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346991732&partnerID=8YFLogxK

U2 - 10.1128/JB.186.1.262-265.2004

DO - 10.1128/JB.186.1.262-265.2004

M3 - Article

VL - 186

SP - 262

EP - 265

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 1

ER -