An isolated tumor perfusion model in mice

Annique M M J Duyverman, Mitsutomo Kohno, Sylvie Roberge, Dai Fukumura, Dan G. Duda, Rakesh K. Jain

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The role of stromal cells in the tumor microenvironment has been extensively characterized. We and others have shown that stromal cells may participate in several steps of the metastatic cascade. This protocol describes an isolated tumor perfusion model that enables studies of cancer and stromal cell shedding. It could also be used to study the effects of therapies interfering with the shedding of tumor cells or fragments, circulating (stem) cells or biomarkers. Primary tumors are grown in a microenvironment in which stromal cells express GFP ubiquitously. Tumors are implanted orthotopically or can be implanted ectopically. As a result, all tumor-associated stromal cells express GFP. This technique can be used to detect and study the role of stromal cells in tumor fragments within the circulation in mice. Studying the role of stromal cells in circulating tumor fragments using this model may take 2-10 weeks, depending on the growth rate of the primary tumor.

Original languageEnglish
Pages (from-to)749-755
Number of pages7
JournalNature Protocols
Volume7
Issue number4
DOIs
Publication statusPublished - 2012 Apr

Fingerprint

Tumors
Stromal Cells
Perfusion
Neoplasms
Tumor Microenvironment
Biomarkers
Stem cells
Stem Cells
Cells
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Duyverman, A. M. M. J., Kohno, M., Roberge, S., Fukumura, D., Duda, D. G., & Jain, R. K. (2012). An isolated tumor perfusion model in mice. Nature Protocols, 7(4), 749-755. https://doi.org/10.1038/nprot.2012.030

An isolated tumor perfusion model in mice. / Duyverman, Annique M M J; Kohno, Mitsutomo; Roberge, Sylvie; Fukumura, Dai; Duda, Dan G.; Jain, Rakesh K.

In: Nature Protocols, Vol. 7, No. 4, 04.2012, p. 749-755.

Research output: Contribution to journalArticle

Duyverman, AMMJ, Kohno, M, Roberge, S, Fukumura, D, Duda, DG & Jain, RK 2012, 'An isolated tumor perfusion model in mice', Nature Protocols, vol. 7, no. 4, pp. 749-755. https://doi.org/10.1038/nprot.2012.030
Duyverman AMMJ, Kohno M, Roberge S, Fukumura D, Duda DG, Jain RK. An isolated tumor perfusion model in mice. Nature Protocols. 2012 Apr;7(4):749-755. https://doi.org/10.1038/nprot.2012.030
Duyverman, Annique M M J ; Kohno, Mitsutomo ; Roberge, Sylvie ; Fukumura, Dai ; Duda, Dan G. ; Jain, Rakesh K. / An isolated tumor perfusion model in mice. In: Nature Protocols. 2012 ; Vol. 7, No. 4. pp. 749-755.
@article{51e431f7b33c44eba29201fb215b8a68,
title = "An isolated tumor perfusion model in mice",
abstract = "The role of stromal cells in the tumor microenvironment has been extensively characterized. We and others have shown that stromal cells may participate in several steps of the metastatic cascade. This protocol describes an isolated tumor perfusion model that enables studies of cancer and stromal cell shedding. It could also be used to study the effects of therapies interfering with the shedding of tumor cells or fragments, circulating (stem) cells or biomarkers. Primary tumors are grown in a microenvironment in which stromal cells express GFP ubiquitously. Tumors are implanted orthotopically or can be implanted ectopically. As a result, all tumor-associated stromal cells express GFP. This technique can be used to detect and study the role of stromal cells in tumor fragments within the circulation in mice. Studying the role of stromal cells in circulating tumor fragments using this model may take 2-10 weeks, depending on the growth rate of the primary tumor.",
author = "Duyverman, {Annique M M J} and Mitsutomo Kohno and Sylvie Roberge and Dai Fukumura and Duda, {Dan G.} and Jain, {Rakesh K.}",
year = "2012",
month = "4",
doi = "10.1038/nprot.2012.030",
language = "English",
volume = "7",
pages = "749--755",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - An isolated tumor perfusion model in mice

AU - Duyverman, Annique M M J

AU - Kohno, Mitsutomo

AU - Roberge, Sylvie

AU - Fukumura, Dai

AU - Duda, Dan G.

AU - Jain, Rakesh K.

PY - 2012/4

Y1 - 2012/4

N2 - The role of stromal cells in the tumor microenvironment has been extensively characterized. We and others have shown that stromal cells may participate in several steps of the metastatic cascade. This protocol describes an isolated tumor perfusion model that enables studies of cancer and stromal cell shedding. It could also be used to study the effects of therapies interfering with the shedding of tumor cells or fragments, circulating (stem) cells or biomarkers. Primary tumors are grown in a microenvironment in which stromal cells express GFP ubiquitously. Tumors are implanted orthotopically or can be implanted ectopically. As a result, all tumor-associated stromal cells express GFP. This technique can be used to detect and study the role of stromal cells in tumor fragments within the circulation in mice. Studying the role of stromal cells in circulating tumor fragments using this model may take 2-10 weeks, depending on the growth rate of the primary tumor.

AB - The role of stromal cells in the tumor microenvironment has been extensively characterized. We and others have shown that stromal cells may participate in several steps of the metastatic cascade. This protocol describes an isolated tumor perfusion model that enables studies of cancer and stromal cell shedding. It could also be used to study the effects of therapies interfering with the shedding of tumor cells or fragments, circulating (stem) cells or biomarkers. Primary tumors are grown in a microenvironment in which stromal cells express GFP ubiquitously. Tumors are implanted orthotopically or can be implanted ectopically. As a result, all tumor-associated stromal cells express GFP. This technique can be used to detect and study the role of stromal cells in tumor fragments within the circulation in mice. Studying the role of stromal cells in circulating tumor fragments using this model may take 2-10 weeks, depending on the growth rate of the primary tumor.

UR - http://www.scopus.com/inward/record.url?scp=84861791171&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861791171&partnerID=8YFLogxK

U2 - 10.1038/nprot.2012.030

DO - 10.1038/nprot.2012.030

M3 - Article

C2 - 22441293

AN - SCOPUS:84861791171

VL - 7

SP - 749

EP - 755

JO - Nature Protocols

JF - Nature Protocols

SN - 1754-2189

IS - 4

ER -