An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling

Yoshika Hayakawa-Yano, Satoshi Suyama, Masahiro Nogami, Masato Yugami, Ikuko Koya, Takako Furukawa, Li Zhou, Manabu Abe, Kenji Sakimura, Hirohide Takebayashi, Atsushi Nakanishi, Hideyuki Okano, Masato Yano

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cell type-specific transcriptomes are enabled by the action of multiple regulators, which are frequently expressed within restricted tissue regions. In the present study, we identify one such regulator, Quaking 5 (Qki5), as an RNA-binding protein (RNABP) that is expressed in early embryonic neural stem cells and subsequently down-regulated during neurogenesis. mRNA sequencing analysis in neural stem cell culture indicates that Qki proteins play supporting roles in the neural stem cell transcriptome and various forms of mRNA processing that may result from regionally restricted expression and subcellular localization. Also, our in utero electroporation gain-of-function study suggests that the nuclear-type Qki isoform Qki5 supports the neural stem cell state. We next performed in vivo transcriptome-wide protein–RNA interaction mapping to search for direct targets of Qki5 and elucidate how Qki5 regulates neural stem cell function. Combined with our transcriptome analysis, this mapping analysis yielded a bona fide map of Qki5–RNA interaction at single-nucleotide resolution, the identification of 892 Qki5 direct target genes, and an accurate Qki5-dependent alternative splicing rule in the developing brain. Last, our target gene list provides the first compelling evidence that Qki5 is associated with specific biological events; namely, cell–cell adhesion. This prediction was confirmed by histological analysis of mice in which Qki proteins were genetically ablated, which revealed disruption of the apical surface of the lateral wall in the developing brain. These data collectively indicate that Qki5 regulates communication between neural stem cells by mediating numerous RNA processing events and suggest new links between splicing regulation and neural stem cell states.

Original languageEnglish
Pages (from-to)1910-1925
Number of pages16
JournalGenes and Development
Volume31
Issue number18
DOIs
Publication statusPublished - 2017 Sep 15

Fingerprint

RNA-Binding Proteins
Neural Stem Cells
RNA Precursors
Embryonic Stem Cells
Cell Adhesion
Transcriptome
Messenger RNA
Electroporation
Neurogenesis
Brain
Alternative Splicing
Gene Expression Profiling
Genes
Protein Isoforms
Proteins
Nucleotides
Cell Culture Techniques
Communication
RNA

Keywords

  • Alternative splicing
  • Cell adhesion
  • HITS-CLIP
  • Neural stem cell
  • Quaking
  • RNA-binding protein

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Hayakawa-Yano, Y., Suyama, S., Nogami, M., Yugami, M., Koya, I., Furukawa, T., ... Yano, M. (2017). An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. Genes and Development, 31(18), 1910-1925. https://doi.org/10.1101/gad.300822.117

An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. / Hayakawa-Yano, Yoshika; Suyama, Satoshi; Nogami, Masahiro; Yugami, Masato; Koya, Ikuko; Furukawa, Takako; Zhou, Li; Abe, Manabu; Sakimura, Kenji; Takebayashi, Hirohide; Nakanishi, Atsushi; Okano, Hideyuki; Yano, Masato.

In: Genes and Development, Vol. 31, No. 18, 15.09.2017, p. 1910-1925.

Research output: Contribution to journalArticle

Hayakawa-Yano, Y, Suyama, S, Nogami, M, Yugami, M, Koya, I, Furukawa, T, Zhou, L, Abe, M, Sakimura, K, Takebayashi, H, Nakanishi, A, Okano, H & Yano, M 2017, 'An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling', Genes and Development, vol. 31, no. 18, pp. 1910-1925. https://doi.org/10.1101/gad.300822.117
Hayakawa-Yano, Yoshika ; Suyama, Satoshi ; Nogami, Masahiro ; Yugami, Masato ; Koya, Ikuko ; Furukawa, Takako ; Zhou, Li ; Abe, Manabu ; Sakimura, Kenji ; Takebayashi, Hirohide ; Nakanishi, Atsushi ; Okano, Hideyuki ; Yano, Masato. / An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling. In: Genes and Development. 2017 ; Vol. 31, No. 18. pp. 1910-1925.
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