Analysis of a novel defective HTLV-I provirus and detection of a new HTLV-I-induced cellular transcript

Satoshi Kubota, Rika A. Furuta, Haruhiko Siomi, Masatoshi Maki, Masakazu Hatanaka

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

HTLV-I generally integrates at least one full-length copy in adult T-cell leukemia (ATL) cells. A group of patients without full-length provirus have a unique conserved truncation of the provirus which retains env-pX-3′LTR. Tumor cells of a patient from this group were genetically analyzed. Analysis of the 5′ and 3′ cellular flanking region adjacent to the provirus suggest that the defective provirus was integrated immediately downstream of a promoter of an unknown cellular gene. The activity of the promoter was weak but was responsive to Tax-like HTLV-I LTR. The provirus may have utilized it as a substitute for the 5′LTR and thus 3′LTR may have become an alternative promoter for the cellular gene, which may give similar viral-cellular interactions to that of general cases with full-length proviruses. Surprisingly, the 3′ cellular flanking region which is thought to be controlled originally by the promoter is constitutively expressed specifically in an HTLV-I producing ATL cell line HUT102G, in which the corresponding region is not modified by provirus. The detection of this HTLV-I-induced transcript provides a probe to find an HTLV-I inducible unknown cellular gene that may be related to the pathogenesis of ATL.

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalFEBS Letters
Volume375
Issue number1-2
DOIs
Publication statusPublished - 1995 Nov 13
Externally publishedYes

Keywords

  • Adult T-cell leukemia
  • HTLV-I
  • Oncogenesis
  • Provirus
  • Tax

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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