Analysis of amino acid and codon usage in Paramecium bursaria

Hideo Dohra, Masahiro Fujishima, Haruo Suzuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The ciliate Paramecium bursaria harbors the green-alga Chlorella symbionts. We reassembled the P. bursaria transcriptome to minimize falsely fused transcripts, and investigated amino acid and codon usage using the transcriptome data. Surface proteins preferentially use smaller amino acid residues like cysteine. Unusual synonymous codon and amino acid usage in highly expressed genes can reflect a balance between translational selection and other factors. A correlation of gene expression level with synonymous codon or amino acid usage is emphasized in genes down-regulated in symbiont-bearing cells compared to symbiont-free cells. Our results imply that the selection is associated with P. bursaria-Chlorella symbiosis.

Original languageEnglish
Article number37343
Pages (from-to)3113-3118
Number of pages6
JournalFEBS Letters
Volume589
Issue number20
DOIs
Publication statusPublished - 2015 Oct 7
Externally publishedYes

Keywords

  • Amino acid usage
  • Gene expression
  • Paramecium bursaria
  • Synonymous codon usage
  • Transcriptome

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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