Analysis of angiogenic profiles by estimation of tumor blood flow with colored dye extraction microspheres after antiangiogenic therapy

Arifumi Iwamaru, Masazumi Watanabe, Takashi Ohtsuka, Hirohisa Horinouchi, Koichi Kobayashi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

No study has directly estimated tumor blood flow after antiangiogenic therapy. Using dye extraction colored microspheres, we estimated blood flow in a rat LY80 tumor model before and after antiangiogenic therapy with TNP-470. Dyed microspheres were infused into the tumor-bearing rats. The dye was extracted and its concentration was quantified by spectrophotometry. Tumor blood flow corrected for wet weight (ml·min-1g-1) was calculated as follows (AU = absorbency units): (blood flow to tumor) = (AU per gram of tumor) x (reference withdrawal rate)/(AU per gram of reference blood). Tumor tissues with or without TNP-470 were also examined histologically. Tumor blood flow 1 week after transplantation could be predicted by the simple regression equation. In the groups treated with TNP-470, this simple regression equation shifted to the left, despite virtually no difference in the results of pathological examination. In LY80 tumors 2 weeks after transplantation, blood flow in tumors treated with TNP-470 was higher than without TNP-470. This technique can be used to obtain information about the angiogenic status within tumor before and after antiangiogenic therapy. These angiogenic profiles may provide important clues to optimal antiangiogenic therapy, combinations of antiangiogenic treatment and cytotoxic therapy, and schedules for combination therapy.

Original languageEnglish
Pages (from-to)1127-1131
Number of pages5
JournalOncology reports
Volume10
Issue number5
DOIs
Publication statusPublished - 2003 Sep

Keywords

  • Angiogenic profiles
  • Antiangiogenic therapy
  • Colored dye extraction microspheres
  • Combination therapy
  • TNP-470
  • Tumor blood flow

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Analysis of angiogenic profiles by estimation of tumor blood flow with colored dye extraction microspheres after antiangiogenic therapy'. Together they form a unique fingerprint.

  • Cite this