Analysis of benzimidazole anthelmintics in livestock foods by HPLC/MS/MS

Shigemi Kai, Takeshi Akaboshi, Michiko Kishi, Hideko Kanazawa, Shizuko Kobayashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A simple, selective and sensitive liquid chromato-graphic ion-trap mass spectrometry (LC-MS-MS) method was developed for the determination of benzimidazole anthelmintics, albendazole, 5-propyl-sulfonyl-1H-benzimidazole-2- amine, flubendazole, mebendazole, oxibendazole, thiabendazole, 5-hydroxy-thiabendazole, and triclabendazole, 5-chloro-6-(2,3-dichloro-phenoxy)- benzimidazole-2-one in livestock foods. LC separation was carried out on a Discovery HS F5 column (150 x 2.1 mm) by using 0.2% formic acid- acetonitrile (50:50) as the mobile phase at a flow rate of 0.2 ml/min. Detection was carried out on an ion-trap mass spectrometer by a multiple reaction monitoring (MRM) mode and an enhanced product ion (EPI) mode via electrospray ionization (ESI). The calibration graphs for benzimidazole anthelmintics were rectilinear from 0.01 to 10 ng with the MRM mode. The precursor ion and product ions obtained under the EPI mode were key criteria to confirm the identity of benzimidazole anthelmintics in samples. The EPI mode detection limits of benzimidazole anthelmintics were 0.005 to 0.05 ng.

Original languageEnglish
Pages (from-to)775-782
Number of pages8
JournalBunseki Kagaku
Volume54
Issue number9
DOIs
Publication statusPublished - 2005

Fingerprint

Anthelmintics
Agriculture
Ions
Thiabendazole
formic acid
triclabendazole
Mebendazole
Albendazole
Electrospray ionization
Monitoring
Mass spectrometers
benzimidazole
Amines
Mass spectrometry
Flow rate
Calibration
Liquids

Keywords

  • Anthelmintics
  • Benzimidazole
  • Liquid chromatographic ion trap mass spectrometry (LC-MS-MS)
  • Livestock foods

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Analysis of benzimidazole anthelmintics in livestock foods by HPLC/MS/MS. / Kai, Shigemi; Akaboshi, Takeshi; Kishi, Michiko; Kanazawa, Hideko; Kobayashi, Shizuko.

In: Bunseki Kagaku, Vol. 54, No. 9, 2005, p. 775-782.

Research output: Contribution to journalArticle

Kai, Shigemi ; Akaboshi, Takeshi ; Kishi, Michiko ; Kanazawa, Hideko ; Kobayashi, Shizuko. / Analysis of benzimidazole anthelmintics in livestock foods by HPLC/MS/MS. In: Bunseki Kagaku. 2005 ; Vol. 54, No. 9. pp. 775-782.
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