Analysis of CSK homologous kinase (CHK/HYL) in hematopoiesis by utilizing gene knockout mice

Isao Hamaguchi, Naoto Yamaguchi, Junko Suda, Atsushi Iwama, Atsushi Hirao, Motohiro Hashiyama, Shin Ichi Aizawa, Toshio Suda

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

CHK/HYL is a non-receptor tyrosine kinase that belongs to CSK (C-terminal Src kinase) family, Northern blotting and RT-PCR analyses showed that CHK/HYL was expressed in large spectrum of hematopoietic cells except for erythroid cells and brain. To explore the function of CHK/HYL in hematopoietic cells, we generated CHK/HYL deficient mice. The mutant mice were apparently normal and fertile, while CSK knockout mice died until E11.5 from a defect in the neural tube formation. Hematological observations including blood counts and FAGS analysis showed no significant abnormalities in CHK/HYL mutant mice. CHK/HYL did not affect the activity of Src, Hck, and Fgr in cultured bone marrow cells, although CSK negatively regulates Src family kinases. These results suggest that CHK/HYL might not have the same function as CSK.

Original languageEnglish
Pages (from-to)172-179
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume224
Issue number1
DOIs
Publication statusPublished - 1996 Jul 5

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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