Analysis of CSK homologous kinase (CHK/HYL) in hematopoiesis by utilizing gene knockout mice

Isao Hamaguchi, Naoto Yamaguchi, Junko Suda, Atsushi Iwama, Atsushi Hirao, Motohiro Hashiyama, Shin Ichi Aizawa, Toshio Suda

Research output: Contribution to journalArticle

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Abstract

CHK/HYL is a non-receptor tyrosine kinase that belongs to CSK (C-terminal Src kinase) family, Northern blotting and RT-PCR analyses showed that CHK/HYL was expressed in large spectrum of hematopoietic cells except for erythroid cells and brain. To explore the function of CHK/HYL in hematopoietic cells, we generated CHK/HYL deficient mice. The mutant mice were apparently normal and fertile, while CSK knockout mice died until E11.5 from a defect in the neural tube formation. Hematological observations including blood counts and FAGS analysis showed no significant abnormalities in CHK/HYL mutant mice. CHK/HYL did not affect the activity of Src, Hck, and Fgr in cultured bone marrow cells, although CSK negatively regulates Src family kinases. These results suggest that CHK/HYL might not have the same function as CSK.

Original languageEnglish
Pages (from-to)172-179
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume224
Issue number1
DOIs
Publication statusPublished - 1996 Jul 5

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Gene Knockout Techniques
Hematopoiesis
Knockout Mice
Phosphotransferases
Genes
Cells
Erythroid Cells
Neural Tube Defects
src-Family Kinases
Bone Marrow Cells
Northern Blotting
Protein-Tyrosine Kinases
Cultured Cells
Brain
Bone
Blood
Polymerase Chain Reaction
Defects
CSK tyrosine-protein kinase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Analysis of CSK homologous kinase (CHK/HYL) in hematopoiesis by utilizing gene knockout mice. / Hamaguchi, Isao; Yamaguchi, Naoto; Suda, Junko; Iwama, Atsushi; Hirao, Atsushi; Hashiyama, Motohiro; Aizawa, Shin Ichi; Suda, Toshio.

In: Biochemical and Biophysical Research Communications, Vol. 224, No. 1, 05.07.1996, p. 172-179.

Research output: Contribution to journalArticle

Hamaguchi, I, Yamaguchi, N, Suda, J, Iwama, A, Hirao, A, Hashiyama, M, Aizawa, SI & Suda, T 1996, 'Analysis of CSK homologous kinase (CHK/HYL) in hematopoiesis by utilizing gene knockout mice', Biochemical and Biophysical Research Communications, vol. 224, no. 1, pp. 172-179. https://doi.org/10.1006/bbrc.1996.1003
Hamaguchi, Isao ; Yamaguchi, Naoto ; Suda, Junko ; Iwama, Atsushi ; Hirao, Atsushi ; Hashiyama, Motohiro ; Aizawa, Shin Ichi ; Suda, Toshio. / Analysis of CSK homologous kinase (CHK/HYL) in hematopoiesis by utilizing gene knockout mice. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 224, No. 1. pp. 172-179.
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