Analysis of ent-kaurenoic acid by ultra-performance liquid chromatography-tandem mass spectrometry

Sho Miyazaki, Honoka Kimura, Masahiro Natsume, Tadao Asami, Ken Ichiro Hayashi, Hiroshi Kawaide, Masatoshi Nakajima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

ent-Kaurenoic acid (KA) is a key intermediate connected to a phytohormone gibberellin. To date, the general procedure for quantifying KA is by using traditional gas chromatography-mass spectrometry (GC-MS). In contrast, gibberellins, which are more hydrophilic than KA, can be easily quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this study, we have established a new method to quantify KA by LC-MS/MS by taking advantage of a key feature of KA, namely the lack of fragmentation that occurs in MS/MS when electrospray ionization (ESI) is in the negative mode. Q1 and Q3 were adopted as identical channels for the multiple reaction monitoring of KA. The method was validated by comparing with the results obtained by selected ion monitoring in GC-MS. This new method could be applicable for the quantification of other hydrophobic compounds.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalBiochemistry and Biophysics Reports
Volume2
DOIs
Publication statusPublished - 2015 Jul 1

Keywords

  • Ent-Kaurenoic acid
  • Gibberellin
  • LC-MS/MS
  • MRM
  • Moss
  • Phytoalexin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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