TY - JOUR
T1 - Analysis of human TIE2 function on hematopoietic stem cells in umbilical cord blood
AU - Yuasa, Hiromi
AU - Takakura, Nobuyuki
AU - Shimomura, Taizou
AU - Suenobu, Souichi
AU - Yamada, Taketo
AU - Nagayama, Hitomi
AU - Oike, Yuichi
AU - Suda, Toshio
N1 - Funding Information:
We thank Dr. Kazuo Matsui in Fukuda Hospital in Kumamoto City for providing us umbilical cord blood. The work was supported by grants-in-aid for Scientific Research on Priority Areas from the Ministry of Education, Science and Culture of Japan.
PY - 2002
Y1 - 2002
N2 - To investigate the behavior of hematopoietic stem cells (HSCs) in cord blood (CB), we analyzed the expression and function of TIE2, a tyrosine kinase receptor. A subpopulation of Lineage (Lin)-/lowCD34+ cells in CB expressed TIE2 (18.8%). Assays for long-term culture-initiating cells (LTC-IC) and cobble-stone formation revealed that Lin-/lowCD34+TIE2+ cells showed to have a capacity of primitive hematopoietic precursor cells in vitro. When Lin-/lowCD34+TIE2+ cells were cultured on the stromal cells, they transmigrated under the stromal layers and kept an immature character for a few weeks. By contrast, Lin-/lowCD34+TIE2- cells differentiated immediately within a few weeks. Finally, we confirmed that 1 × 104 Lin-/lowCD34+TIE2+ cells were engrafted in nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice, while 1 × 104 Lin-/lowCD34+TIE2- cells were not. Taken together, we conclude that TIE2 is a marker of HSCs in CB. A ligand for TIE2, Ang-1 promoted the adhesion of sorted primary Lin-/lowCD34+TIE2+ cells to fibronectin (FN), and this adhesion may play a critical role in keeping HSCs in an immature status under the stromal cells.
AB - To investigate the behavior of hematopoietic stem cells (HSCs) in cord blood (CB), we analyzed the expression and function of TIE2, a tyrosine kinase receptor. A subpopulation of Lineage (Lin)-/lowCD34+ cells in CB expressed TIE2 (18.8%). Assays for long-term culture-initiating cells (LTC-IC) and cobble-stone formation revealed that Lin-/lowCD34+TIE2+ cells showed to have a capacity of primitive hematopoietic precursor cells in vitro. When Lin-/lowCD34+TIE2+ cells were cultured on the stromal cells, they transmigrated under the stromal layers and kept an immature character for a few weeks. By contrast, Lin-/lowCD34+TIE2- cells differentiated immediately within a few weeks. Finally, we confirmed that 1 × 104 Lin-/lowCD34+TIE2+ cells were engrafted in nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice, while 1 × 104 Lin-/lowCD34+TIE2- cells were not. Taken together, we conclude that TIE2 is a marker of HSCs in CB. A ligand for TIE2, Ang-1 promoted the adhesion of sorted primary Lin-/lowCD34+TIE2+ cells to fibronectin (FN), and this adhesion may play a critical role in keeping HSCs in an immature status under the stromal cells.
KW - Cell adhesion
KW - Hematopoietic progenitors
KW - Proliferation
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U2 - 10.1016/S0006-291X(02)02524-X
DO - 10.1016/S0006-291X(02)02524-X
M3 - Article
C2 - 12419314
AN - SCOPUS:0036434254
SN - 0006-291X
VL - 298
SP - 731
EP - 737
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 5
ER -
