Analysis of human TIE2 function on hematopoietic stem cells in umbilical cord blood

Hiromi Yuasa, Nobuyuki Takakura, Taizou Shimomura, Souichi Suenobu, Taketo Yamada, Hitomi Nagayama, Yuichi Oike, Toshio Suda

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To investigate the behavior of hematopoietic stem cells (HSCs) in cord blood (CB), we analyzed the expression and function of TIE2, a tyrosine kinase receptor. A subpopulation of Lineage (Lin)-/lowCD34+ cells in CB expressed TIE2 (18.8%). Assays for long-term culture-initiating cells (LTC-IC) and cobble-stone formation revealed that Lin-/lowCD34+TIE2+ cells showed to have a capacity of primitive hematopoietic precursor cells in vitro. When Lin-/lowCD34+TIE2+ cells were cultured on the stromal cells, they transmigrated under the stromal layers and kept an immature character for a few weeks. By contrast, Lin-/lowCD34+TIE2- cells differentiated immediately within a few weeks. Finally, we confirmed that 1 × 104 Lin-/lowCD34+TIE2+ cells were engrafted in nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice, while 1 × 104 Lin-/lowCD34+TIE2- cells were not. Taken together, we conclude that TIE2 is a marker of HSCs in CB. A ligand for TIE2, Ang-1 promoted the adhesion of sorted primary Lin-/lowCD34+TIE2+ cells to fibronectin (FN), and this adhesion may play a critical role in keeping HSCs in an immature status under the stromal cells.

Original languageEnglish
Pages (from-to)731-737
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume298
Issue number5
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • Cell adhesion
  • Hematopoietic progenitors
  • Proliferation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Yuasa, H., Takakura, N., Shimomura, T., Suenobu, S., Yamada, T., Nagayama, H., Oike, Y., & Suda, T. (2002). Analysis of human TIE2 function on hematopoietic stem cells in umbilical cord blood. Biochemical and Biophysical Research Communications, 298(5), 731-737. https://doi.org/10.1016/S0006-291X(02)02524-X