TY - JOUR
T1 - Analysis of melatonin using a pH- and temperature-responsive aqueous chromatography system
AU - Ayano, Eri
AU - Suzuki, Yusuke
AU - Kanezawa, Masahito
AU - Sakamoto, Chikako
AU - Morita-Murase, Yuko
AU - Nagata, Yoshiko
AU - Kanazawa, Hideko
AU - Kikuchi, Akihiko
AU - Okano, Teruo
PY - 2007/7/13
Y1 - 2007/7/13
N2 - A new method for the qualitative and quantitative analysis of an intracerebral hormone, such as melatonin, has been proposed, utilizing newly designed copolymers that include ion-exchange groups. These copolymers responded to both the temperature and the pH, and the copolymers were modified with cross-linked hydrogel applied onto aminopropyl silica beads. The products were evaluated as HPLC packing materials for a pH- and temperature-responsive chromatography. The property of the surface of the stationary phase was altered from hydrophilic to hydrophobic, and from charged to non-charged by changes in both the temperature and the pH. In the chromatographic system, we investigated how to change the retention of melatonin by varying the temperature. A pH- and temperature-responsive chromatography is expected to be useful for the separation of pharmaceuticals and biomolecules.
AB - A new method for the qualitative and quantitative analysis of an intracerebral hormone, such as melatonin, has been proposed, utilizing newly designed copolymers that include ion-exchange groups. These copolymers responded to both the temperature and the pH, and the copolymers were modified with cross-linked hydrogel applied onto aminopropyl silica beads. The products were evaluated as HPLC packing materials for a pH- and temperature-responsive chromatography. The property of the surface of the stationary phase was altered from hydrophilic to hydrophobic, and from charged to non-charged by changes in both the temperature and the pH. In the chromatographic system, we investigated how to change the retention of melatonin by varying the temperature. A pH- and temperature-responsive chromatography is expected to be useful for the separation of pharmaceuticals and biomolecules.
KW - HPLC
KW - Intracerebral hormone
KW - Melatonin
KW - Poly(N-isopropylacrylamide)
KW - pH- and temperature-responsive chromatography
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U2 - 10.1016/j.chroma.2007.01.063
DO - 10.1016/j.chroma.2007.01.063
M3 - Article
C2 - 17292374
AN - SCOPUS:34250683485
SN - 0021-9673
VL - 1156
SP - 213
EP - 219
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2 SPEC. ISS.
ER -