Analysis of microRNAs expressions in chondrosarcoma

Teruhito Yoshitaka, Akira Kawai, Shigeru Miyaki, Kunihiko Numoto, Kazutaka Kikuta, Toshifumi Ozaki, Martin Lotz, Hiroshi Asahara

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55 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs capable of inhibiting gene expression post-transcriptionally and expression profiling can provide therapeutic targets and tools for cancer diagnosis. Chondrosarcoma is a mesenchymal tumor with unknown cause and differentiation status. Here, we profiled miRNA expression of chondrosarcoma, namely clinical samples from human conventional chondrosarcoma tissue, established chondrosarcoma cell lines, and primary non-tumorous adult articular chondrocytes, by miRNA array and quantitative real-time PCR. A wide variety of miRNAs were differently downregulated in chondrosarcoma compared to non-tumorous articular chondrocytes; 27 miRNAs: miR-10b, 23b, 24-1, 27b, 100, 134, 136, 136, 138, 181d, 186, 193b, 221, 222, 335, 337-5p, 376a, 376a, 376b, 376c, 377, 454, 495, 497, 505, 574-3p, and 660, were significantly downregulated in chondrosarcoma and only 2: miR-96 and 183, were upregulated. We further validated the expression levels of miRNAs by quantitative real-time PCR for miR-181a, let-7a, 100, 222, 136, 376a, and 335 in extended number of chondrosarcoma clinical samples. Among them, all except miR-181a were found to be significantly downregulated in chondrosarcoma derived samples. The findings provide potential diagnostic value and new molecular understanding of chondrosarcoma.

Original languageEnglish
Pages (from-to)1992-1998
Number of pages7
JournalJournal of Orthopaedic Research
Volume31
Issue number12
DOIs
Publication statusPublished - 2013

Keywords

  • cartilage
  • chondrosarcoma
  • malignancy
  • microRNA
  • sarcoma

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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    Yoshitaka, T., Kawai, A., Miyaki, S., Numoto, K., Kikuta, K., Ozaki, T., Lotz, M., & Asahara, H. (2013). Analysis of microRNAs expressions in chondrosarcoma. Journal of Orthopaedic Research, 31(12), 1992-1998. https://doi.org/10.1002/jor.22457