Analysis of microRNAs expressions in chondrosarcoma

Teruhito Yoshitaka, Akira Kawai, Shigeru Miyaki, Kunihiko Numoto, Kazutaka Kikuta, Toshifumi Ozaki, Martin Lotz, Hiroshi Asahara

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs capable of inhibiting gene expression post-transcriptionally and expression profiling can provide therapeutic targets and tools for cancer diagnosis. Chondrosarcoma is a mesenchymal tumor with unknown cause and differentiation status. Here, we profiled miRNA expression of chondrosarcoma, namely clinical samples from human conventional chondrosarcoma tissue, established chondrosarcoma cell lines, and primary non-tumorous adult articular chondrocytes, by miRNA array and quantitative real-time PCR. A wide variety of miRNAs were differently downregulated in chondrosarcoma compared to non-tumorous articular chondrocytes; 27 miRNAs: miR-10b, 23b, 24-1, 27b, 100, 134, 136, 136, 138, 181d, 186, 193b, 221, 222, 335, 337-5p, 376a, 376a, 376b, 376c, 377, 454, 495, 497, 505, 574-3p, and 660, were significantly downregulated in chondrosarcoma and only 2: miR-96 and 183, were upregulated. We further validated the expression levels of miRNAs by quantitative real-time PCR for miR-181a, let-7a, 100, 222, 136, 376a, and 335 in extended number of chondrosarcoma clinical samples. Among them, all except miR-181a were found to be significantly downregulated in chondrosarcoma derived samples. The findings provide potential diagnostic value and new molecular understanding of chondrosarcoma.

Original languageEnglish
Pages (from-to)1992-1998
Number of pages7
JournalJournal of Orthopaedic Research
Volume31
Issue number12
DOIs
Publication statusPublished - 2013
Externally publishedYes

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Chondrosarcoma
MicroRNAs
Down-Regulation
Chondrocytes
Real-Time Polymerase Chain Reaction
Joints
Small Untranslated RNA
Neoplasms
Gene Expression
Cell Line

Keywords

  • cartilage
  • chondrosarcoma
  • malignancy
  • microRNA
  • sarcoma

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Yoshitaka, T., Kawai, A., Miyaki, S., Numoto, K., Kikuta, K., Ozaki, T., ... Asahara, H. (2013). Analysis of microRNAs expressions in chondrosarcoma. Journal of Orthopaedic Research, 31(12), 1992-1998. https://doi.org/10.1002/jor.22457

Analysis of microRNAs expressions in chondrosarcoma. / Yoshitaka, Teruhito; Kawai, Akira; Miyaki, Shigeru; Numoto, Kunihiko; Kikuta, Kazutaka; Ozaki, Toshifumi; Lotz, Martin; Asahara, Hiroshi.

In: Journal of Orthopaedic Research, Vol. 31, No. 12, 2013, p. 1992-1998.

Research output: Contribution to journalArticle

Yoshitaka, T, Kawai, A, Miyaki, S, Numoto, K, Kikuta, K, Ozaki, T, Lotz, M & Asahara, H 2013, 'Analysis of microRNAs expressions in chondrosarcoma', Journal of Orthopaedic Research, vol. 31, no. 12, pp. 1992-1998. https://doi.org/10.1002/jor.22457
Yoshitaka T, Kawai A, Miyaki S, Numoto K, Kikuta K, Ozaki T et al. Analysis of microRNAs expressions in chondrosarcoma. Journal of Orthopaedic Research. 2013;31(12):1992-1998. https://doi.org/10.1002/jor.22457
Yoshitaka, Teruhito ; Kawai, Akira ; Miyaki, Shigeru ; Numoto, Kunihiko ; Kikuta, Kazutaka ; Ozaki, Toshifumi ; Lotz, Martin ; Asahara, Hiroshi. / Analysis of microRNAs expressions in chondrosarcoma. In: Journal of Orthopaedic Research. 2013 ; Vol. 31, No. 12. pp. 1992-1998.
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