Analysis of pathogenicity by restriction-endonuclease digestion of amplified genomic DNA of Entamoeba histolytica isolated in Pernambuco, Brazil

Hiroshi Tachibana, Seiki Kobayashi, Kilma C. Paz, Ivanize S. Aca, Seiki Tateno, Seiji Ihara

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The pathogenicity of 47 strains of Entamoeba histolytica isolated in Pernambuco, Brazil, was examined using the polymerase chain reaction (PCR) followed by restriction-endonuclease digestion. Electrophoretic patterns of PCR products digested with HinfI revealed that all strains were nonpathogenic. The results were entirely in accord with phenotypic properties such as isoenzyme patterns and the failure to bind a pathogenic-isolatespecific monoclonal antibody. When the sensitivity of PCR was examined, amplified products could be detected from template DNA equivalent to five trophozoites. These observations indicate that PCR amplification of genomic DNA and subsequent restriction-enzyme digestion is a useful strategy for obtaining a sensitive and accurate diagnosis. The present study also demonstrates that nonpathogenic strains of E. histolytica predominate in northeastern Brazil.

Original languageEnglish
Pages (from-to)433-436
Number of pages4
JournalParasitology Research
Volume78
Issue number5
DOIs
Publication statusPublished - 1992 May

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Entamoeba histolytica
DNA Restriction Enzymes
restriction endonucleases
Brazil
Virulence
Digestion
pathogenicity
digestion
polymerase chain reaction
genomics
Polymerase Chain Reaction
DNA
Trophozoites
trophozoites
avirulent strains
Isoenzymes
isozymes
monoclonal antibodies
Monoclonal Antibodies
enzymes

ASJC Scopus subject areas

  • Parasitology

Cite this

Analysis of pathogenicity by restriction-endonuclease digestion of amplified genomic DNA of Entamoeba histolytica isolated in Pernambuco, Brazil. / Tachibana, Hiroshi; Kobayashi, Seiki; Paz, Kilma C.; Aca, Ivanize S.; Tateno, Seiki; Ihara, Seiji.

In: Parasitology Research, Vol. 78, No. 5, 05.1992, p. 433-436.

Research output: Contribution to journalArticle

Tachibana, Hiroshi ; Kobayashi, Seiki ; Paz, Kilma C. ; Aca, Ivanize S. ; Tateno, Seiki ; Ihara, Seiji. / Analysis of pathogenicity by restriction-endonuclease digestion of amplified genomic DNA of Entamoeba histolytica isolated in Pernambuco, Brazil. In: Parasitology Research. 1992 ; Vol. 78, No. 5. pp. 433-436.
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