Analysis of repetitive amino acid motifs reveals the essential features of spider dragline silk proteins

Ali D. Malay, Kazuharu Arakawa, Keiji Numata

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The extraordinary mechanical properties of spider dragline silk are dependent on the highly repetitive sequences of the component proteins, major ampullate spidroin 1 and 2 (MaSp2 and MaSp2). MaSp sequences are dominated by repetitive modules composed of short amino acid motifs; however, the patterns of motif conservation through evolution and their relevance to silk characteristics are not well understood. We performed a systematic analysis of MaSp sequences encompassing infraorder Araneomorphae based on the conservation of explicitly defined motifs, with the aim of elucidating the essential elements of MaSp1 and MaSp2. The results show that the GGY motif is nearly ubiquitous in the two types of MaSp, while MaSp2 is invariably associated with GP and di-glutamine (QQ) motifs. Further analysis revealed an extended MaSp2 consensus sequence in family Araneidae, with implications for the classification of the archetypal spidroins ADF3 and ADF4. Additionally, the analysis of RNA-seq data showed the expression of a set of distinct MaSp-like variants in genus Tetragnatha. Finally, an apparent association was uncovered between web architecture and the abundance of GP, QQ, and GGY motifs in MaSp2, which suggests a co-expansion of these motifs in response to the evolution of spiders’ prey capture strategy.

Original languageEnglish
Article numbere0183397
JournalPLoS One
Volume12
Issue number8
DOIs
Publication statusPublished - 2017 Aug 1

Fingerprint

silk proteins
amino acid motifs
Amino Acid Motifs
Spiders
Silk
Nucleic Acid Repetitive Sequences
silk
Araneae
Conservation
Tetragnatha
Fibroins
Amino Acids
Araneidae
repetitive sequences
consensus sequence
Consensus Sequence
Glutamine
glutamine
mechanical properties
Sequence Analysis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Analysis of repetitive amino acid motifs reveals the essential features of spider dragline silk proteins. / Malay, Ali D.; Arakawa, Kazuharu; Numata, Keiji.

In: PLoS One, Vol. 12, No. 8, e0183397, 01.08.2017.

Research output: Contribution to journalArticle

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