Analysis of serum FSH bioactivity in a patient with an FSH-secreting pituitary microadenoma and multicystic ovaries: A case report

T. Kajitani, S. Liu, Tetsuo Maruyama, Hiroshi Uchida, R. Sakurai, Hirotaka Masuda, T. Nagashima, M. Ono, T. Arase, Y. Yoshimura

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

FSH-secreting pituitary adenoma (FSHoma) is often associated with increased levels of serum FSH and ovarian hyperstimulation syndrome (OHSS). The OHSS has historically been attributed to elevated FSH production by the FSHoma; however, some FSHoma patients with OHSS have normal serum FSH levels. OHSS may result not from increased FSH levels, but also from increased bioactivity of the FSH derived from the adenoma. To address this, we measured the FSH bioactivity in the serum of a 40-year-old woman with an FSHoma and OHSS, whose FSH levels were normal. Chinese hamster ovary cells stably expressing FSH receptors were prepared and transfected with a cAMP-responsive element-driven luciferase reporter plasmid. Cells were then treated with recombinant human FSH (rhFSH), the patient's sera, or sera from controls, collected at different time points, and subjected to a luciferase assay. Luciferase activity was increased in response to rhFSH in a dose-dependent manner. The responsiveness was further augmented by co-addition of a 3-methyl isobutylxanthine, which improved the sensitivity of our assay. Unexpectedly, the serum FSH bioactivity/immunoactivity ratio of the patient was mostly equal to that of normal subjects. This was confirmed with a granulosa cell aromatase assay. This case report suggests that alternate explanations may exist for the OHSS phenotype seen in some FSHoma patients.

Original languageEnglish
Pages (from-to)435-439
Number of pages5
JournalHuman Reproduction
Volume23
Issue number2
DOIs
Publication statusPublished - 2008 Feb

Fingerprint

Ovarian Hyperstimulation Syndrome
Ovary
Luciferases
Serum
Human Follicle Stimulating Hormone
FSH Receptors
Aromatase
Granulosa Cells
Pituitary Neoplasms
Cricetulus
Adenoma
Plasmids
Phenotype

Keywords

  • Bioactivity
  • FSH
  • Ovarian hyperstimulation
  • Pituitary adenoma

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Medicine(all)

Cite this

Analysis of serum FSH bioactivity in a patient with an FSH-secreting pituitary microadenoma and multicystic ovaries : A case report. / Kajitani, T.; Liu, S.; Maruyama, Tetsuo; Uchida, Hiroshi; Sakurai, R.; Masuda, Hirotaka; Nagashima, T.; Ono, M.; Arase, T.; Yoshimura, Y.

In: Human Reproduction, Vol. 23, No. 2, 02.2008, p. 435-439.

Research output: Contribution to journalArticle

Kajitani, T. ; Liu, S. ; Maruyama, Tetsuo ; Uchida, Hiroshi ; Sakurai, R. ; Masuda, Hirotaka ; Nagashima, T. ; Ono, M. ; Arase, T. ; Yoshimura, Y. / Analysis of serum FSH bioactivity in a patient with an FSH-secreting pituitary microadenoma and multicystic ovaries : A case report. In: Human Reproduction. 2008 ; Vol. 23, No. 2. pp. 435-439.
@article{e4ea7bcd362948548c2055024066958a,
title = "Analysis of serum FSH bioactivity in a patient with an FSH-secreting pituitary microadenoma and multicystic ovaries: A case report",
abstract = "FSH-secreting pituitary adenoma (FSHoma) is often associated with increased levels of serum FSH and ovarian hyperstimulation syndrome (OHSS). The OHSS has historically been attributed to elevated FSH production by the FSHoma; however, some FSHoma patients with OHSS have normal serum FSH levels. OHSS may result not from increased FSH levels, but also from increased bioactivity of the FSH derived from the adenoma. To address this, we measured the FSH bioactivity in the serum of a 40-year-old woman with an FSHoma and OHSS, whose FSH levels were normal. Chinese hamster ovary cells stably expressing FSH receptors were prepared and transfected with a cAMP-responsive element-driven luciferase reporter plasmid. Cells were then treated with recombinant human FSH (rhFSH), the patient's sera, or sera from controls, collected at different time points, and subjected to a luciferase assay. Luciferase activity was increased in response to rhFSH in a dose-dependent manner. The responsiveness was further augmented by co-addition of a 3-methyl isobutylxanthine, which improved the sensitivity of our assay. Unexpectedly, the serum FSH bioactivity/immunoactivity ratio of the patient was mostly equal to that of normal subjects. This was confirmed with a granulosa cell aromatase assay. This case report suggests that alternate explanations may exist for the OHSS phenotype seen in some FSHoma patients.",
keywords = "Bioactivity, FSH, Ovarian hyperstimulation, Pituitary adenoma",
author = "T. Kajitani and S. Liu and Tetsuo Maruyama and Hiroshi Uchida and R. Sakurai and Hirotaka Masuda and T. Nagashima and M. Ono and T. Arase and Y. Yoshimura",
year = "2008",
month = "2",
doi = "10.1093/humrep/dem374",
language = "English",
volume = "23",
pages = "435--439",
journal = "Human Reproduction",
issn = "0268-1161",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Analysis of serum FSH bioactivity in a patient with an FSH-secreting pituitary microadenoma and multicystic ovaries

T2 - A case report

AU - Kajitani, T.

AU - Liu, S.

AU - Maruyama, Tetsuo

AU - Uchida, Hiroshi

AU - Sakurai, R.

AU - Masuda, Hirotaka

AU - Nagashima, T.

AU - Ono, M.

AU - Arase, T.

AU - Yoshimura, Y.

PY - 2008/2

Y1 - 2008/2

N2 - FSH-secreting pituitary adenoma (FSHoma) is often associated with increased levels of serum FSH and ovarian hyperstimulation syndrome (OHSS). The OHSS has historically been attributed to elevated FSH production by the FSHoma; however, some FSHoma patients with OHSS have normal serum FSH levels. OHSS may result not from increased FSH levels, but also from increased bioactivity of the FSH derived from the adenoma. To address this, we measured the FSH bioactivity in the serum of a 40-year-old woman with an FSHoma and OHSS, whose FSH levels were normal. Chinese hamster ovary cells stably expressing FSH receptors were prepared and transfected with a cAMP-responsive element-driven luciferase reporter plasmid. Cells were then treated with recombinant human FSH (rhFSH), the patient's sera, or sera from controls, collected at different time points, and subjected to a luciferase assay. Luciferase activity was increased in response to rhFSH in a dose-dependent manner. The responsiveness was further augmented by co-addition of a 3-methyl isobutylxanthine, which improved the sensitivity of our assay. Unexpectedly, the serum FSH bioactivity/immunoactivity ratio of the patient was mostly equal to that of normal subjects. This was confirmed with a granulosa cell aromatase assay. This case report suggests that alternate explanations may exist for the OHSS phenotype seen in some FSHoma patients.

AB - FSH-secreting pituitary adenoma (FSHoma) is often associated with increased levels of serum FSH and ovarian hyperstimulation syndrome (OHSS). The OHSS has historically been attributed to elevated FSH production by the FSHoma; however, some FSHoma patients with OHSS have normal serum FSH levels. OHSS may result not from increased FSH levels, but also from increased bioactivity of the FSH derived from the adenoma. To address this, we measured the FSH bioactivity in the serum of a 40-year-old woman with an FSHoma and OHSS, whose FSH levels were normal. Chinese hamster ovary cells stably expressing FSH receptors were prepared and transfected with a cAMP-responsive element-driven luciferase reporter plasmid. Cells were then treated with recombinant human FSH (rhFSH), the patient's sera, or sera from controls, collected at different time points, and subjected to a luciferase assay. Luciferase activity was increased in response to rhFSH in a dose-dependent manner. The responsiveness was further augmented by co-addition of a 3-methyl isobutylxanthine, which improved the sensitivity of our assay. Unexpectedly, the serum FSH bioactivity/immunoactivity ratio of the patient was mostly equal to that of normal subjects. This was confirmed with a granulosa cell aromatase assay. This case report suggests that alternate explanations may exist for the OHSS phenotype seen in some FSHoma patients.

KW - Bioactivity

KW - FSH

KW - Ovarian hyperstimulation

KW - Pituitary adenoma

UR - http://www.scopus.com/inward/record.url?scp=40349094731&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40349094731&partnerID=8YFLogxK

U2 - 10.1093/humrep/dem374

DO - 10.1093/humrep/dem374

M3 - Article

C2 - 18056718

AN - SCOPUS:40349094731

VL - 23

SP - 435

EP - 439

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 2

ER -