Analysis of the expression and function of BRINP family genes during neuronal differentiation in mouse embryonic stem cell-derived neural stem cells

Michiyo Terashima, Miwako Kobayashi, Makoto Motomiya, Nobuo Inoue, Tetsu Yoshida, Hideyuki Okano, Norimasa Iwasaki, Akio Minami, Ichiro Matsuoka

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We previously identified a novel family of genes, BRINP1, 2, and 3, that are predominantly and widely expressed in both the central nervous system (CNS) and peripheral nervous system (PNS). In the present study, we analyzed the expression pattern of three BRINP genes during differentiation of mouse embryonic stem (ES) cell-derived neural stem cells (NSCs) and their effects on the cell-cycle regulation of NSCs. While there was no significant expression of any BRINP-mRNA expressed in mouse ES cells, BRINP 1 and 2-mRNAs was expressed at high levels in the ES cell-derived neural stem cells. Upon differentiation into neuronal cells in the presence of retinoic acid and BDNF, all three types of BRINP-mRNA were induced with a similar time course peaking at day three of treatment. Upon differentiation into astroglial cells in the presence of serum, BRINP1-mRNA was slightly up-regulated, while BRINP2- and BRINP3-mRNAs were almost abolished in the astrocytes. While 69.2, 26.1, and 7.7% of cells in a population of NSCs in the exponentially growing phase were in the G1, S and G2 phases, respectively, over-expression of any one of the three BRINP genes completely abolished cells in the G2 phase and significantly reduced the cells in S phase to 11.8-13.8%. Based on these results, the physiological roles of induced BRINP genes in the cell-cycle suppression of terminally differentiated post-mitotic neurons are discussed.

Original languageEnglish
Pages (from-to)1387-1393
Number of pages7
JournalJournal of Neuroscience Research
Volume88
Issue number7
DOIs
Publication statusPublished - 2010 May 15

Fingerprint

Neural Stem Cells
Messenger RNA
Genes
G2 Phase
S Phase
cdc Genes
Brain-Derived Neurotrophic Factor
Peripheral Nervous System
G1 Phase
Embryonic Stem Cells
Tretinoin
Astrocytes
Cell Cycle
Central Nervous System
Mouse Embryonic Stem Cells
Neurons
Serum
Population

Keywords

  • BRINP family
  • Cell cycle
  • Mouse embryonic stem cell
  • Neural stem cell
  • Neuronal differentiation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Analysis of the expression and function of BRINP family genes during neuronal differentiation in mouse embryonic stem cell-derived neural stem cells. / Terashima, Michiyo; Kobayashi, Miwako; Motomiya, Makoto; Inoue, Nobuo; Yoshida, Tetsu; Okano, Hideyuki; Iwasaki, Norimasa; Minami, Akio; Matsuoka, Ichiro.

In: Journal of Neuroscience Research, Vol. 88, No. 7, 15.05.2010, p. 1387-1393.

Research output: Contribution to journalArticle

Terashima, Michiyo ; Kobayashi, Miwako ; Motomiya, Makoto ; Inoue, Nobuo ; Yoshida, Tetsu ; Okano, Hideyuki ; Iwasaki, Norimasa ; Minami, Akio ; Matsuoka, Ichiro. / Analysis of the expression and function of BRINP family genes during neuronal differentiation in mouse embryonic stem cell-derived neural stem cells. In: Journal of Neuroscience Research. 2010 ; Vol. 88, No. 7. pp. 1387-1393.
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AU - Inoue, Nobuo

AU - Yoshida, Tetsu

AU - Okano, Hideyuki

AU - Iwasaki, Norimasa

AU - Minami, Akio

AU - Matsuoka, Ichiro

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AB - We previously identified a novel family of genes, BRINP1, 2, and 3, that are predominantly and widely expressed in both the central nervous system (CNS) and peripheral nervous system (PNS). In the present study, we analyzed the expression pattern of three BRINP genes during differentiation of mouse embryonic stem (ES) cell-derived neural stem cells (NSCs) and their effects on the cell-cycle regulation of NSCs. While there was no significant expression of any BRINP-mRNA expressed in mouse ES cells, BRINP 1 and 2-mRNAs was expressed at high levels in the ES cell-derived neural stem cells. Upon differentiation into neuronal cells in the presence of retinoic acid and BDNF, all three types of BRINP-mRNA were induced with a similar time course peaking at day three of treatment. Upon differentiation into astroglial cells in the presence of serum, BRINP1-mRNA was slightly up-regulated, while BRINP2- and BRINP3-mRNAs were almost abolished in the astrocytes. While 69.2, 26.1, and 7.7% of cells in a population of NSCs in the exponentially growing phase were in the G1, S and G2 phases, respectively, over-expression of any one of the three BRINP genes completely abolished cells in the G2 phase and significantly reduced the cells in S phase to 11.8-13.8%. Based on these results, the physiological roles of induced BRINP genes in the cell-cycle suppression of terminally differentiated post-mitotic neurons are discussed.

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