Analysis of the promoter region of human placenta-specific DSCR4 gene

Satoko Asai, Akiko Yamaki, Jun Kudoh, Nobuyoshi Shimizu, Yoshiko Shimizu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The gene DSCR4 locates in the band q22.2 of human chromosome 21 and encodes a protein of 118 amino acids. Expression of DSCR4 is restricted to human placenta and placental choriocarcinoma cell lines BeWo and JEG3. The 5′-RACE method using RNA from human placenta indicated the major transcription start site at 93 nt upstream (nt - 93) of the initiation codon. Transfection assay using a series of deletion constructs of the 5′-flanking region fused to the luciferase reporter gene identified three positive regions nt - 2200 to - 2088, nt - 2064 to - 1924, nt - 810 to - 632 and two negative regions nt - 1923 to - 1740, nt - 631 to - 425. The computer analysis predicted the presence of several cis-elements in these regions and the promoter assay using various mutants of consensus sequence identified two distinct cis-elements for OLF-1 and E47. The electrophoretic mobility shift assay (EMSA) using the extracts of DSCR4-expressing cells confirmed the binding of certain protein factors to these cis-elements. In fact, OLF-1-like transcription factor, EBF-3 and EBF-4 were detected in the DSCR4-expressing cell lines and human placenta. Based on these data, we postulated that transcription of DSCR4 gene is regulated positively by binding of OLF-1-like transcription factor and negatively by binding of E47-like transcription factor.

Original languageEnglish
Pages (from-to)40-50
Number of pages11
JournalBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume1779
Issue number1
DOIs
Publication statusPublished - 2008 Jan 1

Keywords

  • Choriocarcinoma
  • DSCR4
  • E47
  • EMSA
  • Luciferase
  • OLF-1

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics

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