Analysis of the promoter region of human placenta-specific DSCR4 gene

Satoko Asai, Akiko Yamaki, Jun Kudo, Nobuyoshi Shimizu, Yoshiko Shimizu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The gene DSCR4 locates in the band q22.2 of human chromosome 21 and encodes a protein of 118 amino acids. Expression of DSCR4 is restricted to human placenta and placental choriocarcinoma cell lines BeWo and JEG3. The 5′-RACE method using RNA from human placenta indicated the major transcription start site at 93 nt upstream (nt - 93) of the initiation codon. Transfection assay using a series of deletion constructs of the 5′-flanking region fused to the luciferase reporter gene identified three positive regions nt - 2200 to - 2088, nt - 2064 to - 1924, nt - 810 to - 632 and two negative regions nt - 1923 to - 1740, nt - 631 to - 425. The computer analysis predicted the presence of several cis-elements in these regions and the promoter assay using various mutants of consensus sequence identified two distinct cis-elements for OLF-1 and E47. The electrophoretic mobility shift assay (EMSA) using the extracts of DSCR4-expressing cells confirmed the binding of certain protein factors to these cis-elements. In fact, OLF-1-like transcription factor, EBF-3 and EBF-4 were detected in the DSCR4-expressing cell lines and human placenta. Based on these data, we postulated that transcription of DSCR4 gene is regulated positively by binding of OLF-1-like transcription factor and negatively by binding of E47-like transcription factor.

Original languageEnglish
Pages (from-to)40-50
Number of pages11
JournalBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume1779
Issue number1
DOIs
Publication statusPublished - 2008 Jan

Fingerprint

Genetic Promoter Regions
Placenta
Assays
Transcription Factors
Genes
Cells
Transcription Factor 3
Cell Line
Chromosomes, Human, Pair 21
Electrophoretic mobility
Choriocarcinoma
Initiator Codon
5' Flanking Region
Transcription Initiation Site
Consensus Sequence
Human Chromosomes
Electrophoretic Mobility Shift Assay
Transcription
Chromosomes
Luciferases

Keywords

  • Choriocarcinoma
  • DSCR4
  • E47
  • EMSA
  • Luciferase
  • OLF-1

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Analysis of the promoter region of human placenta-specific DSCR4 gene. / Asai, Satoko; Yamaki, Akiko; Kudo, Jun; Shimizu, Nobuyoshi; Shimizu, Yoshiko.

In: Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, Vol. 1779, No. 1, 01.2008, p. 40-50.

Research output: Contribution to journalArticle

Asai, Satoko ; Yamaki, Akiko ; Kudo, Jun ; Shimizu, Nobuyoshi ; Shimizu, Yoshiko. / Analysis of the promoter region of human placenta-specific DSCR4 gene. In: Biochimica et Biophysica Acta - Gene Regulatory Mechanisms. 2008 ; Vol. 1779, No. 1. pp. 40-50.
@article{9410594b597145d1968a7351424c559e,
title = "Analysis of the promoter region of human placenta-specific DSCR4 gene",
abstract = "The gene DSCR4 locates in the band q22.2 of human chromosome 21 and encodes a protein of 118 amino acids. Expression of DSCR4 is restricted to human placenta and placental choriocarcinoma cell lines BeWo and JEG3. The 5′-RACE method using RNA from human placenta indicated the major transcription start site at 93 nt upstream (nt - 93) of the initiation codon. Transfection assay using a series of deletion constructs of the 5′-flanking region fused to the luciferase reporter gene identified three positive regions nt - 2200 to - 2088, nt - 2064 to - 1924, nt - 810 to - 632 and two negative regions nt - 1923 to - 1740, nt - 631 to - 425. The computer analysis predicted the presence of several cis-elements in these regions and the promoter assay using various mutants of consensus sequence identified two distinct cis-elements for OLF-1 and E47. The electrophoretic mobility shift assay (EMSA) using the extracts of DSCR4-expressing cells confirmed the binding of certain protein factors to these cis-elements. In fact, OLF-1-like transcription factor, EBF-3 and EBF-4 were detected in the DSCR4-expressing cell lines and human placenta. Based on these data, we postulated that transcription of DSCR4 gene is regulated positively by binding of OLF-1-like transcription factor and negatively by binding of E47-like transcription factor.",
keywords = "Choriocarcinoma, DSCR4, E47, EMSA, Luciferase, OLF-1",
author = "Satoko Asai and Akiko Yamaki and Jun Kudo and Nobuyoshi Shimizu and Yoshiko Shimizu",
year = "2008",
month = "1",
doi = "10.1016/j.bbagrm.2007.09.005",
language = "English",
volume = "1779",
pages = "40--50",
journal = "Biochimica et Biophysica Acta - Gene Regulatory Mechanisms",
issn = "1874-9399",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Analysis of the promoter region of human placenta-specific DSCR4 gene

AU - Asai, Satoko

AU - Yamaki, Akiko

AU - Kudo, Jun

AU - Shimizu, Nobuyoshi

AU - Shimizu, Yoshiko

PY - 2008/1

Y1 - 2008/1

N2 - The gene DSCR4 locates in the band q22.2 of human chromosome 21 and encodes a protein of 118 amino acids. Expression of DSCR4 is restricted to human placenta and placental choriocarcinoma cell lines BeWo and JEG3. The 5′-RACE method using RNA from human placenta indicated the major transcription start site at 93 nt upstream (nt - 93) of the initiation codon. Transfection assay using a series of deletion constructs of the 5′-flanking region fused to the luciferase reporter gene identified three positive regions nt - 2200 to - 2088, nt - 2064 to - 1924, nt - 810 to - 632 and two negative regions nt - 1923 to - 1740, nt - 631 to - 425. The computer analysis predicted the presence of several cis-elements in these regions and the promoter assay using various mutants of consensus sequence identified two distinct cis-elements for OLF-1 and E47. The electrophoretic mobility shift assay (EMSA) using the extracts of DSCR4-expressing cells confirmed the binding of certain protein factors to these cis-elements. In fact, OLF-1-like transcription factor, EBF-3 and EBF-4 were detected in the DSCR4-expressing cell lines and human placenta. Based on these data, we postulated that transcription of DSCR4 gene is regulated positively by binding of OLF-1-like transcription factor and negatively by binding of E47-like transcription factor.

AB - The gene DSCR4 locates in the band q22.2 of human chromosome 21 and encodes a protein of 118 amino acids. Expression of DSCR4 is restricted to human placenta and placental choriocarcinoma cell lines BeWo and JEG3. The 5′-RACE method using RNA from human placenta indicated the major transcription start site at 93 nt upstream (nt - 93) of the initiation codon. Transfection assay using a series of deletion constructs of the 5′-flanking region fused to the luciferase reporter gene identified three positive regions nt - 2200 to - 2088, nt - 2064 to - 1924, nt - 810 to - 632 and two negative regions nt - 1923 to - 1740, nt - 631 to - 425. The computer analysis predicted the presence of several cis-elements in these regions and the promoter assay using various mutants of consensus sequence identified two distinct cis-elements for OLF-1 and E47. The electrophoretic mobility shift assay (EMSA) using the extracts of DSCR4-expressing cells confirmed the binding of certain protein factors to these cis-elements. In fact, OLF-1-like transcription factor, EBF-3 and EBF-4 were detected in the DSCR4-expressing cell lines and human placenta. Based on these data, we postulated that transcription of DSCR4 gene is regulated positively by binding of OLF-1-like transcription factor and negatively by binding of E47-like transcription factor.

KW - Choriocarcinoma

KW - DSCR4

KW - E47

KW - EMSA

KW - Luciferase

KW - OLF-1

UR - http://www.scopus.com/inward/record.url?scp=39449126561&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39449126561&partnerID=8YFLogxK

U2 - 10.1016/j.bbagrm.2007.09.005

DO - 10.1016/j.bbagrm.2007.09.005

M3 - Article

C2 - 18086574

AN - SCOPUS:39449126561

VL - 1779

SP - 40

EP - 50

JO - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms

JF - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms

SN - 1874-9399

IS - 1

ER -