Analysis of the survival of mature human eosinophils: Interleukin-5 prevents apoptosis in mature human eosinophils

Yuji Yamagychi, Toshio Suda, Shigeo Ohta, Kaoru Tominaga, Yasusada Miura, Tadashi Kasahara

Research output: Contribution to journalArticle

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Abstract

We and other groups have previously shown that interleukin-5 (IL-5) maintained the viability of mature eosinophils in an in vitro liquid culture system. Mature eosinophils did not proliferate but their survival was maintained in the presence of IL-5. Using this culture system, we investigated the mechanism of IL-5-mediated survival. In the absence of human IL-S (hlL-5) mature eosinophils succumbed after 4 days, while in the presence of hIL-5 they survived up to 10 days. When DNA extracts of cultured eosinophils were analyzed on an agar gel electrophoresis, marked DIMA fragmentation was observed in the absence of hIL-5, while no significant DNA fragmentation was observed in the culture with hIL-5 for 48 hours. The DNA fragmentation appeared as early as 6 to 12 hours after hIL-5 deprivation. Concomitantly, IL-5 stimulated total RNA and protein synthesis, but did not induce DNA synthesis in mature eosinophils. Because cycloheximide or actinomycin D impeded the protection of apoptosis by hIL-5, some new RNA and protein synthesis appeared to be required in this phenomena. These findings indicate that IL-5 maintains survival of mature eosinophils with induction of new RNA and protein synthesis, thus leading to the inhibition of apoptosis.

Original languageEnglish
Pages (from-to)2542-2547
Number of pages6
JournalBlood
Volume78
Issue number10
Publication statusPublished - 1991 Nov 15

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Interleukin-5
Survival Analysis
Eosinophils
Apoptosis
DNA
RNA
DNA Fragmentation
Proteins
Dactinomycin
Cycloheximide
Electrophoresis
Survival
Agar Gel Electrophoresis
Agar
Gels
Liquids

ASJC Scopus subject areas

  • Hematology

Cite this

Yamagychi, Y., Suda, T., Ohta, S., Tominaga, K., Miura, Y., & Kasahara, T. (1991). Analysis of the survival of mature human eosinophils: Interleukin-5 prevents apoptosis in mature human eosinophils. Blood, 78(10), 2542-2547.

Analysis of the survival of mature human eosinophils : Interleukin-5 prevents apoptosis in mature human eosinophils. / Yamagychi, Yuji; Suda, Toshio; Ohta, Shigeo; Tominaga, Kaoru; Miura, Yasusada; Kasahara, Tadashi.

In: Blood, Vol. 78, No. 10, 15.11.1991, p. 2542-2547.

Research output: Contribution to journalArticle

Yamagychi, Y, Suda, T, Ohta, S, Tominaga, K, Miura, Y & Kasahara, T 1991, 'Analysis of the survival of mature human eosinophils: Interleukin-5 prevents apoptosis in mature human eosinophils', Blood, vol. 78, no. 10, pp. 2542-2547.
Yamagychi Y, Suda T, Ohta S, Tominaga K, Miura Y, Kasahara T. Analysis of the survival of mature human eosinophils: Interleukin-5 prevents apoptosis in mature human eosinophils. Blood. 1991 Nov 15;78(10):2542-2547.
Yamagychi, Yuji ; Suda, Toshio ; Ohta, Shigeo ; Tominaga, Kaoru ; Miura, Yasusada ; Kasahara, Tadashi. / Analysis of the survival of mature human eosinophils : Interleukin-5 prevents apoptosis in mature human eosinophils. In: Blood. 1991 ; Vol. 78, No. 10. pp. 2542-2547.
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