Analysis of Toll-like receptor 2, 4, 6 and 9 genome DNA mutations in patients with tractable and intractable gastric mucosal diseases

Tadashi Ohara, Tetsuo Morishita, Hiroshi Hashimoto, Masumi Akimoto, Yuhsaku Kanoh, Noriko Nakajima, Hidekazu Suzuki, Toshifumi Hibi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The possible involvement of Toll-like receptor (TLR) genome DNA in the prolongation and relapse of inflammatory intestinal diseases and alcoholic hepatic diseases has been reported. In this study, we examined the relationship of mutations of the TLR 2, 4, 6 and 9 genomic DNA to recurrent or intractable gastritis or gastric ulcers. The subjects were 32 patients, including 6 with H. pylori (Hp)-positive gastritis, 4 with Hp-negative gastritis, 10 with Hp-positive tractable gastric ulcer, 5 with Hp-positive recurrent gastric ulcer after Hp eradication, and 7 with Hp-negative easily recurrent gastric ulcer after Hp eradication. Gastric mucosal tissue and peripheral blood specimens were collected from each of the patients. DNA was extracted from the tissue and blood specimens and subjected to electrophoresis by the PCR method, using the oligonucleotide primers of TLR 2, 4, 6 and 9. The gastric mucosal tissue specimens were collected endoscopically from the sites of the lesions. Subsequently, the presence or absence of genomic DNA mutations in the blood and tissue specimens was examined using a DNA sequencer. TLR 2, 4, 6 or 9 DNA mutations were not observed in any of the gastric mucosal or peripheral blood specimens obtained from patients with tractable gastritis or gastric ulcer, or from those with intractable gastric ulcer who were Hp-positive or Hp-negative or had become Hp-negative after eradication therapy. These data suggest that mutations of the TLR 2, 4, 6 and 9 genome DNA may not be involved in the recurrence, delayed healing or intractability of gastritis and gastric ulcers.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalInternational Journal of Molecular Medicine
Volume17
Issue number1
Publication statusPublished - 2006 Jan

Fingerprint

Toll-Like Receptor 9
Stomach Diseases
Toll-Like Receptor 2
Toll-Like Receptor 4
Pylorus
Stomach Ulcer
Genome
Mutation
DNA
Gastritis
Stomach
Mucous Membrane
Recurrence
Intestinal Diseases
DNA Primers
Toll-Like Receptors
Electrophoresis

Keywords

  • Gastric mucosal diseases
  • Toll-like receptor

ASJC Scopus subject areas

  • Genetics

Cite this

Ohara, T., Morishita, T., Hashimoto, H., Akimoto, M., Kanoh, Y., Nakajima, N., ... Hibi, T. (2006). Analysis of Toll-like receptor 2, 4, 6 and 9 genome DNA mutations in patients with tractable and intractable gastric mucosal diseases. International Journal of Molecular Medicine, 17(1), 53-58.

Analysis of Toll-like receptor 2, 4, 6 and 9 genome DNA mutations in patients with tractable and intractable gastric mucosal diseases. / Ohara, Tadashi; Morishita, Tetsuo; Hashimoto, Hiroshi; Akimoto, Masumi; Kanoh, Yuhsaku; Nakajima, Noriko; Suzuki, Hidekazu; Hibi, Toshifumi.

In: International Journal of Molecular Medicine, Vol. 17, No. 1, 01.2006, p. 53-58.

Research output: Contribution to journalArticle

Ohara, T, Morishita, T, Hashimoto, H, Akimoto, M, Kanoh, Y, Nakajima, N, Suzuki, H & Hibi, T 2006, 'Analysis of Toll-like receptor 2, 4, 6 and 9 genome DNA mutations in patients with tractable and intractable gastric mucosal diseases', International Journal of Molecular Medicine, vol. 17, no. 1, pp. 53-58.
Ohara, Tadashi ; Morishita, Tetsuo ; Hashimoto, Hiroshi ; Akimoto, Masumi ; Kanoh, Yuhsaku ; Nakajima, Noriko ; Suzuki, Hidekazu ; Hibi, Toshifumi. / Analysis of Toll-like receptor 2, 4, 6 and 9 genome DNA mutations in patients with tractable and intractable gastric mucosal diseases. In: International Journal of Molecular Medicine. 2006 ; Vol. 17, No. 1. pp. 53-58.
@article{98404be749d147a1a83f0b9f5b841215,
title = "Analysis of Toll-like receptor 2, 4, 6 and 9 genome DNA mutations in patients with tractable and intractable gastric mucosal diseases",
abstract = "The possible involvement of Toll-like receptor (TLR) genome DNA in the prolongation and relapse of inflammatory intestinal diseases and alcoholic hepatic diseases has been reported. In this study, we examined the relationship of mutations of the TLR 2, 4, 6 and 9 genomic DNA to recurrent or intractable gastritis or gastric ulcers. The subjects were 32 patients, including 6 with H. pylori (Hp)-positive gastritis, 4 with Hp-negative gastritis, 10 with Hp-positive tractable gastric ulcer, 5 with Hp-positive recurrent gastric ulcer after Hp eradication, and 7 with Hp-negative easily recurrent gastric ulcer after Hp eradication. Gastric mucosal tissue and peripheral blood specimens were collected from each of the patients. DNA was extracted from the tissue and blood specimens and subjected to electrophoresis by the PCR method, using the oligonucleotide primers of TLR 2, 4, 6 and 9. The gastric mucosal tissue specimens were collected endoscopically from the sites of the lesions. Subsequently, the presence or absence of genomic DNA mutations in the blood and tissue specimens was examined using a DNA sequencer. TLR 2, 4, 6 or 9 DNA mutations were not observed in any of the gastric mucosal or peripheral blood specimens obtained from patients with tractable gastritis or gastric ulcer, or from those with intractable gastric ulcer who were Hp-positive or Hp-negative or had become Hp-negative after eradication therapy. These data suggest that mutations of the TLR 2, 4, 6 and 9 genome DNA may not be involved in the recurrence, delayed healing or intractability of gastritis and gastric ulcers.",
keywords = "Gastric mucosal diseases, Toll-like receptor",
author = "Tadashi Ohara and Tetsuo Morishita and Hiroshi Hashimoto and Masumi Akimoto and Yuhsaku Kanoh and Noriko Nakajima and Hidekazu Suzuki and Toshifumi Hibi",
year = "2006",
month = "1",
language = "English",
volume = "17",
pages = "53--58",
journal = "International Journal of Molecular Medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "1",

}

TY - JOUR

T1 - Analysis of Toll-like receptor 2, 4, 6 and 9 genome DNA mutations in patients with tractable and intractable gastric mucosal diseases

AU - Ohara, Tadashi

AU - Morishita, Tetsuo

AU - Hashimoto, Hiroshi

AU - Akimoto, Masumi

AU - Kanoh, Yuhsaku

AU - Nakajima, Noriko

AU - Suzuki, Hidekazu

AU - Hibi, Toshifumi

PY - 2006/1

Y1 - 2006/1

N2 - The possible involvement of Toll-like receptor (TLR) genome DNA in the prolongation and relapse of inflammatory intestinal diseases and alcoholic hepatic diseases has been reported. In this study, we examined the relationship of mutations of the TLR 2, 4, 6 and 9 genomic DNA to recurrent or intractable gastritis or gastric ulcers. The subjects were 32 patients, including 6 with H. pylori (Hp)-positive gastritis, 4 with Hp-negative gastritis, 10 with Hp-positive tractable gastric ulcer, 5 with Hp-positive recurrent gastric ulcer after Hp eradication, and 7 with Hp-negative easily recurrent gastric ulcer after Hp eradication. Gastric mucosal tissue and peripheral blood specimens were collected from each of the patients. DNA was extracted from the tissue and blood specimens and subjected to electrophoresis by the PCR method, using the oligonucleotide primers of TLR 2, 4, 6 and 9. The gastric mucosal tissue specimens were collected endoscopically from the sites of the lesions. Subsequently, the presence or absence of genomic DNA mutations in the blood and tissue specimens was examined using a DNA sequencer. TLR 2, 4, 6 or 9 DNA mutations were not observed in any of the gastric mucosal or peripheral blood specimens obtained from patients with tractable gastritis or gastric ulcer, or from those with intractable gastric ulcer who were Hp-positive or Hp-negative or had become Hp-negative after eradication therapy. These data suggest that mutations of the TLR 2, 4, 6 and 9 genome DNA may not be involved in the recurrence, delayed healing or intractability of gastritis and gastric ulcers.

AB - The possible involvement of Toll-like receptor (TLR) genome DNA in the prolongation and relapse of inflammatory intestinal diseases and alcoholic hepatic diseases has been reported. In this study, we examined the relationship of mutations of the TLR 2, 4, 6 and 9 genomic DNA to recurrent or intractable gastritis or gastric ulcers. The subjects were 32 patients, including 6 with H. pylori (Hp)-positive gastritis, 4 with Hp-negative gastritis, 10 with Hp-positive tractable gastric ulcer, 5 with Hp-positive recurrent gastric ulcer after Hp eradication, and 7 with Hp-negative easily recurrent gastric ulcer after Hp eradication. Gastric mucosal tissue and peripheral blood specimens were collected from each of the patients. DNA was extracted from the tissue and blood specimens and subjected to electrophoresis by the PCR method, using the oligonucleotide primers of TLR 2, 4, 6 and 9. The gastric mucosal tissue specimens were collected endoscopically from the sites of the lesions. Subsequently, the presence or absence of genomic DNA mutations in the blood and tissue specimens was examined using a DNA sequencer. TLR 2, 4, 6 or 9 DNA mutations were not observed in any of the gastric mucosal or peripheral blood specimens obtained from patients with tractable gastritis or gastric ulcer, or from those with intractable gastric ulcer who were Hp-positive or Hp-negative or had become Hp-negative after eradication therapy. These data suggest that mutations of the TLR 2, 4, 6 and 9 genome DNA may not be involved in the recurrence, delayed healing or intractability of gastritis and gastric ulcers.

KW - Gastric mucosal diseases

KW - Toll-like receptor

UR - http://www.scopus.com/inward/record.url?scp=33644874980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644874980&partnerID=8YFLogxK

M3 - Article

VL - 17

SP - 53

EP - 58

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

IS - 1

ER -