Analysis of ultraviolet-absorbing compounds in human urine by high performance liquid chromatography

Yoshiko Itoh, Y. Yokoyama, S. Nagura, S. Sugata, T. Fujie, Y. Matsushima, N. Takai

Research output: Contribution to journalArticle

Abstract

Ultraviolet (UV)-absorbing compounds in human urine were analyzed by means of high-performance liquid chromatography using the column of macroreticular anion exchange resin maintained at 60 degree C. The sample was eluted with a linear gradient of water to 0. 25 M ammonium perchlorate-acetonitrile (85:15) at 0-50 min and with 0. 25 M ammonium perchlorate-acetonitrile (85:15) at 50-70 min and monitored with absorption at 254 nm. Reproducible chromatograms were obtained and 68 well resoluted peaks were numbered. The storage of a urine sample at minus 20 degree C for 7 weeks did not significantly affect the chromatogram. The correlation coefficients of every pair of the numbered peaks were calculated to examine the daily variations and the individual difference of the UV-absorbing urine components.

Original languageEnglish
Pages (from-to)2993-2998
Number of pages6
JournalJournal of Liquid Chromatography
Volume10
Issue number13
Publication statusPublished - 1987

Fingerprint

High performance liquid chromatography
High Pressure Liquid Chromatography
Urine
Anion Exchange Resins
Individuality
Water
acetonitrile
ammonium perchlorate

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

Itoh, Y., Yokoyama, Y., Nagura, S., Sugata, S., Fujie, T., Matsushima, Y., & Takai, N. (1987). Analysis of ultraviolet-absorbing compounds in human urine by high performance liquid chromatography. Journal of Liquid Chromatography, 10(13), 2993-2998.

Analysis of ultraviolet-absorbing compounds in human urine by high performance liquid chromatography. / Itoh, Yoshiko; Yokoyama, Y.; Nagura, S.; Sugata, S.; Fujie, T.; Matsushima, Y.; Takai, N.

In: Journal of Liquid Chromatography, Vol. 10, No. 13, 1987, p. 2993-2998.

Research output: Contribution to journalArticle

Itoh, Y, Yokoyama, Y, Nagura, S, Sugata, S, Fujie, T, Matsushima, Y & Takai, N 1987, 'Analysis of ultraviolet-absorbing compounds in human urine by high performance liquid chromatography', Journal of Liquid Chromatography, vol. 10, no. 13, pp. 2993-2998.
Itoh, Yoshiko ; Yokoyama, Y. ; Nagura, S. ; Sugata, S. ; Fujie, T. ; Matsushima, Y. ; Takai, N. / Analysis of ultraviolet-absorbing compounds in human urine by high performance liquid chromatography. In: Journal of Liquid Chromatography. 1987 ; Vol. 10, No. 13. pp. 2993-2998.
@article{d6dc65d2cd40470ea377ccb0422b2a7e,
title = "Analysis of ultraviolet-absorbing compounds in human urine by high performance liquid chromatography",
abstract = "Ultraviolet (UV)-absorbing compounds in human urine were analyzed by means of high-performance liquid chromatography using the column of macroreticular anion exchange resin maintained at 60 degree C. The sample was eluted with a linear gradient of water to 0. 25 M ammonium perchlorate-acetonitrile (85:15) at 0-50 min and with 0. 25 M ammonium perchlorate-acetonitrile (85:15) at 50-70 min and monitored with absorption at 254 nm. Reproducible chromatograms were obtained and 68 well resoluted peaks were numbered. The storage of a urine sample at minus 20 degree C for 7 weeks did not significantly affect the chromatogram. The correlation coefficients of every pair of the numbered peaks were calculated to examine the daily variations and the individual difference of the UV-absorbing urine components.",
author = "Yoshiko Itoh and Y. Yokoyama and S. Nagura and S. Sugata and T. Fujie and Y. Matsushima and N. Takai",
year = "1987",
language = "English",
volume = "10",
pages = "2993--2998",
journal = "Journal of Liquid Chromatography and Related Technologies",
issn = "1082-6076",
publisher = "Taylor and Francis Ltd.",
number = "13",

}

TY - JOUR

T1 - Analysis of ultraviolet-absorbing compounds in human urine by high performance liquid chromatography

AU - Itoh, Yoshiko

AU - Yokoyama, Y.

AU - Nagura, S.

AU - Sugata, S.

AU - Fujie, T.

AU - Matsushima, Y.

AU - Takai, N.

PY - 1987

Y1 - 1987

N2 - Ultraviolet (UV)-absorbing compounds in human urine were analyzed by means of high-performance liquid chromatography using the column of macroreticular anion exchange resin maintained at 60 degree C. The sample was eluted with a linear gradient of water to 0. 25 M ammonium perchlorate-acetonitrile (85:15) at 0-50 min and with 0. 25 M ammonium perchlorate-acetonitrile (85:15) at 50-70 min and monitored with absorption at 254 nm. Reproducible chromatograms were obtained and 68 well resoluted peaks were numbered. The storage of a urine sample at minus 20 degree C for 7 weeks did not significantly affect the chromatogram. The correlation coefficients of every pair of the numbered peaks were calculated to examine the daily variations and the individual difference of the UV-absorbing urine components.

AB - Ultraviolet (UV)-absorbing compounds in human urine were analyzed by means of high-performance liquid chromatography using the column of macroreticular anion exchange resin maintained at 60 degree C. The sample was eluted with a linear gradient of water to 0. 25 M ammonium perchlorate-acetonitrile (85:15) at 0-50 min and with 0. 25 M ammonium perchlorate-acetonitrile (85:15) at 50-70 min and monitored with absorption at 254 nm. Reproducible chromatograms were obtained and 68 well resoluted peaks were numbered. The storage of a urine sample at minus 20 degree C for 7 weeks did not significantly affect the chromatogram. The correlation coefficients of every pair of the numbered peaks were calculated to examine the daily variations and the individual difference of the UV-absorbing urine components.

UR - http://www.scopus.com/inward/record.url?scp=0023510865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023510865&partnerID=8YFLogxK

M3 - Article

VL - 10

SP - 2993

EP - 2998

JO - Journal of Liquid Chromatography and Related Technologies

JF - Journal of Liquid Chromatography and Related Technologies

SN - 1082-6076

IS - 13

ER -