Analysis of ultraviolet-absorbing compounds in human urine by high performance liquid chromatography

Ultraviolet(UV)-absorbing compounds in human urine were analyzed by means of high-performance liquid chromatography using the column of macroreticular anion exchange resin maintained at 60°C. The sample was eluted with a linear gradient of water to 0.25 M ammonium perchlorate-acetonitrile (85:15) at 0–50 min and with 0.25 M ammonium perchlorate-acetonitrile (85:15) at 50–70 min and monitored with absorption at 254 nm. Reproducible chromatograms were obtained and 68 well resoluted peaks were numbered. The storage of a urine sample at −20°C for 7 weeks did not significantly affect the chromatogram. The correlation coefficients of every pair of the numbered peaks were calculated to examine the daily variations and the individual difference of the UV-absorbing urine components.