Analysis of unrejoined chromosomal breakage in human fibroblast cells exposed to low- and high-LET radiation.

Honglu Wu, Yoshiya Furusawa, Kerry George, Tetsuya Kawata, Francis A. Cucinotta

Research output: Contribution to journalArticle

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Abstract

Reported studies of DNA breakage induced by radiation of various qualities have generally shown a higher fraction of unrejoined residual breaks after high-LET exposure. This observation is supported by the argument that high-LET radiation induced DNA breaks that are more complex in nature and, thus, less likely to be repaired. In most cases the doses used in these studies were very high. We have studied unrejoined chromosome breaks by analyzing chromosome aberrations using a fluorescence in situ hybridization (FISH) technique with a combination of whole chromosome specific probes and probes specific for the telomere region of the chromosomes. Confluent human fibroblast cells (AG1522) were irradiated with gamma rays, 490 MeV/nucleon Si, or with Fe ions at either 200 and 500 MeV/nucleon, and were allowed to repair at 37 degrees C for 24 hours after exposure. A chemically induced premature chromosome condensation (PCC) technique was used to condense chromosomes in the G2 phase of the cell cycle. Results showed that the frequency of unrejoined chromosome breaks was higher after high-LET radiation, and the ratio of unrejoined to misrejoined chromosome breaks increased steadily with LET up a peak value at 440 keV/microm.

Original languageEnglish
JournalJournal of Radiation Research
Volume43 Suppl
Publication statusPublished - 2002 Dec
Externally publishedYes

Fingerprint

Linear Energy Transfer
Chromosome Breakage
chromosomes
chromosome breakage
fibroblasts
Fibroblasts
Chromosomes
Radiation
radiation
DNA Breaks
chromosome aberrations
G2 Phase
Gamma Rays
telomeres
Telomere
DNA
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
Chromosome Aberrations
interphase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Radiation

Cite this

Analysis of unrejoined chromosomal breakage in human fibroblast cells exposed to low- and high-LET radiation. / Wu, Honglu; Furusawa, Yoshiya; George, Kerry; Kawata, Tetsuya; Cucinotta, Francis A.

In: Journal of Radiation Research, Vol. 43 Suppl, 12.2002.

Research output: Contribution to journalArticle

Wu, Honglu ; Furusawa, Yoshiya ; George, Kerry ; Kawata, Tetsuya ; Cucinotta, Francis A. / Analysis of unrejoined chromosomal breakage in human fibroblast cells exposed to low- and high-LET radiation. In: Journal of Radiation Research. 2002 ; Vol. 43 Suppl.
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