TY - JOUR
T1 - Anatomic site-specific proteomic signatures of gastrointestinal stromal tumors
AU - Suehara, Yoshiyuki
AU - Kikuta, Kazutaka
AU - Nakayama, Robert
AU - Fujii, Kiyonaga
AU - Ichikawa, Hitoshi
AU - Shibata, Tatsuhiro
AU - Seki, Kunihiko
AU - Hasegawa, Tadashi
AU - Gotoh, Masahiro
AU - Tochigi, Naobumi
AU - Shimoda, Tadakazu
AU - Shimada, Yasuhiro
AU - Sano, Takeshi
AU - Beppu, Yasuo
AU - Kurosawa, Hisashi
AU - Hirohashi, Setsuo
AU - Kawai, Akira
AU - Kondo, Tadashi
PY - 2009
Y1 - 2009
N2 - The gastrointestinal stromal tumor (GIST) is the most common mesenchymal malignancy of the gastrointestinal tract. Its clinical course ranges widely from a curable disorder to a highly malignant disease. Although its clinical and molecular characteristics depend on the anatomic site of origin, the molecular background of GIST arising in different anatomical site has not been studied yet. To investigate the proteomic background of GIST, we examined the proteomic features corresponding to the anatomic site of tumor origin. Comparison of the proteomic profile of gastric (23 cases) and small intestinal (9 cases) GIST by 2-DE revealed 105 protein spots with significantly different intensity (p <0.01) between the two groups.Mass spectrometric study identified 68 distinct proteins for these 105 protein spots, including cancer-associated ones such as prohibit in, pigment epithelium-derived factor, and alpha-actinin 4. The intensity of 37/105 (35.2%) protein spots was significantly concordant with the corresponding mRNA levels (p <0.01). Although both 2-D DIGE and microarray experiments showed significant up-regulation of vimentin expression in small intestinal GIST, Western blotting did not show a significant difference between the two groups. In conclusion, our study demonstrates the proteins specially expressed in GIST depending on their site of origin, as well as the unique advantage offered by use of proteomics to acquire such data. The identified proteins may provide clues to understanding the different characteristics of GIST depending on their site of origin.
AB - The gastrointestinal stromal tumor (GIST) is the most common mesenchymal malignancy of the gastrointestinal tract. Its clinical course ranges widely from a curable disorder to a highly malignant disease. Although its clinical and molecular characteristics depend on the anatomic site of origin, the molecular background of GIST arising in different anatomical site has not been studied yet. To investigate the proteomic background of GIST, we examined the proteomic features corresponding to the anatomic site of tumor origin. Comparison of the proteomic profile of gastric (23 cases) and small intestinal (9 cases) GIST by 2-DE revealed 105 protein spots with significantly different intensity (p <0.01) between the two groups.Mass spectrometric study identified 68 distinct proteins for these 105 protein spots, including cancer-associated ones such as prohibit in, pigment epithelium-derived factor, and alpha-actinin 4. The intensity of 37/105 (35.2%) protein spots was significantly concordant with the corresponding mRNA levels (p <0.01). Although both 2-D DIGE and microarray experiments showed significant up-regulation of vimentin expression in small intestinal GIST, Western blotting did not show a significant difference between the two groups. In conclusion, our study demonstrates the proteins specially expressed in GIST depending on their site of origin, as well as the unique advantage offered by use of proteomics to acquire such data. The identified proteins may provide clues to understanding the different characteristics of GIST depending on their site of origin.
KW - 2-D DIGE
KW - Anatomic site
KW - Gastrointestinal stromal tumor
KW - Proteomics
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U2 - 10.1002/prca.200800168
DO - 10.1002/prca.200800168
M3 - Article
AN - SCOPUS:67649438799
VL - 3
SP - 584
EP - 596
JO - Proteomics - Clinical Applications
JF - Proteomics - Clinical Applications
SN - 1862-8346
IS - 5
ER -