Anatomical and surgical evaluation of the common marmoset as an animal model in hearing research

Sho Kurihara, Masato Fujioka, Junichi Hata, Tomohiko Yoshida, Motoki Hirabayashi, Yutaka Yamamoto, Kaoru Ogawa, Hiromi Kojima, Hirotaka James Okano

Research output: Contribution to journalArticle

Abstract

Recent studies have indicated that direct administration of viral vectors or small compounds to the inner ear may aid in the treatment of Sensorineural hearing loss (SNHL). However, due to species differences between humans and rodents, translating experimental results into clinical applications remains challenging. The common marmoset (Callithrix jacchus), a New World monkey, is considered a pre-clinical animal model. In the present study, we describe morphometric data acquired from the temporal bone of the common marmoset in order to define the routes of topical drug administration to the inner ear. Dissection and diffusion tensor tractography (DTT) were performed on the fixed cadaverous heads of 13 common marmosets. To investigate potential routes for drug administration to the inner ear, we explored the anatomy of the round window, oval window (OW), semicircular canal, and endolymphatic sac (ES). Among these, the approach via the round window with posterior tympanotomy appeared feasible for delivering drugs to the inner ear without manipulating the tympanic membrane, minimizing the chances of conductive hearing loss. The courses of four critical nerves [including the facial nerve (FN)] were visualized using three-dimensional (3D) DTT, which may help to avoid nerve damage during surgery. Finally, to investigate the feasibility of actual drug administration, we measured the volume of the round window niche (RWN), which was approximately 0.9 μL. The present findings may help to establish experimental standards for evaluating new therapies in this primate model.

Original languageEnglish
Article number60
JournalFrontiers in Neuroanatomy
Volume13
DOIs
Publication statusPublished - 2019 Jun 3

Fingerprint

Callithrix
Inner Ear
Hearing
Animal Models
Diffusion Tensor Imaging
Research
Drug Administration Routes
Platyrrhini
Endolymphatic Sac
Conductive Hearing Loss
Topical Administration
Semicircular Canals
Tympanic Membrane
Sensorineural Hearing Loss
Temporal Bone
Facial Nerve
Pharmaceutical Preparations
Primates
Dissection
Rodentia

Keywords

  • Common marmoset
  • Diffusion tensor tractography
  • Drug delivery
  • Ear anatomy
  • Temporal bone

ASJC Scopus subject areas

  • Anatomy
  • Neuroscience (miscellaneous)
  • Cellular and Molecular Neuroscience

Cite this

Anatomical and surgical evaluation of the common marmoset as an animal model in hearing research. / Kurihara, Sho; Fujioka, Masato; Hata, Junichi; Yoshida, Tomohiko; Hirabayashi, Motoki; Yamamoto, Yutaka; Ogawa, Kaoru; Kojima, Hiromi; Okano, Hirotaka James.

In: Frontiers in Neuroanatomy, Vol. 13, 60, 03.06.2019.

Research output: Contribution to journalArticle

Kurihara, Sho ; Fujioka, Masato ; Hata, Junichi ; Yoshida, Tomohiko ; Hirabayashi, Motoki ; Yamamoto, Yutaka ; Ogawa, Kaoru ; Kojima, Hiromi ; Okano, Hirotaka James. / Anatomical and surgical evaluation of the common marmoset as an animal model in hearing research. In: Frontiers in Neuroanatomy. 2019 ; Vol. 13.
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