Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22

Ni Putu Desy Aryantini, Daisuke Kondoh, Keita Nishiyama, Yuji Yamamoto, Takao Mukai, I. Nengah Sujaya, Tadasu Urashima, Kenji Fukuda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.

Original languageEnglish
Article numberfnx056
JournalFEMS microbiology letters
Volume364
Issue number6
DOIs
Publication statusPublished - 2017 Mar 1
Externally publishedYes

Fingerprint

Lactobacillus rhamnosus
Laminin
Membrane Proteins
Bacterial Adhesion
Lithium Chloride
Glyceraldehyde-3-Phosphate Dehydrogenases
DNA-Binding Proteins
L-Lactate Dehydrogenase
Extracellular Matrix
Mass Spectrometry
Glycoproteins
Proteins

Keywords

  • Cell surface proteins
  • Host-microbial interactions
  • Lactic acid bacteria
  • Laminin
  • Probiotics
  • Ribosomal proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22. / Aryantini, Ni Putu Desy; Kondoh, Daisuke; Nishiyama, Keita; Yamamoto, Yuji; Mukai, Takao; Sujaya, I. Nengah; Urashima, Tadasu; Fukuda, Kenji.

In: FEMS microbiology letters, Vol. 364, No. 6, fnx056, 01.03.2017.

Research output: Contribution to journalArticle

Aryantini, NPD, Kondoh, D, Nishiyama, K, Yamamoto, Y, Mukai, T, Sujaya, IN, Urashima, T & Fukuda, K 2017, 'Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22', FEMS microbiology letters, vol. 364, no. 6, fnx056. https://doi.org/10.1093/femsle/fnx056
Aryantini, Ni Putu Desy ; Kondoh, Daisuke ; Nishiyama, Keita ; Yamamoto, Yuji ; Mukai, Takao ; Sujaya, I. Nengah ; Urashima, Tadasu ; Fukuda, Kenji. / Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22. In: FEMS microbiology letters. 2017 ; Vol. 364, No. 6.
@article{5b242d82cf1c48229bc4a1336446529c,
title = "Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22",
abstract = "Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.",
keywords = "Cell surface proteins, Host-microbial interactions, Lactic acid bacteria, Laminin, Probiotics, Ribosomal proteins",
author = "Aryantini, {Ni Putu Desy} and Daisuke Kondoh and Keita Nishiyama and Yuji Yamamoto and Takao Mukai and Sujaya, {I. Nengah} and Tadasu Urashima and Kenji Fukuda",
year = "2017",
month = "3",
day = "1",
doi = "10.1093/femsle/fnx056",
language = "English",
volume = "364",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22

AU - Aryantini, Ni Putu Desy

AU - Kondoh, Daisuke

AU - Nishiyama, Keita

AU - Yamamoto, Yuji

AU - Mukai, Takao

AU - Sujaya, I. Nengah

AU - Urashima, Tadasu

AU - Fukuda, Kenji

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.

AB - Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.

KW - Cell surface proteins

KW - Host-microbial interactions

KW - Lactic acid bacteria

KW - Laminin

KW - Probiotics

KW - Ribosomal proteins

UR - http://www.scopus.com/inward/record.url?scp=85019859967&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019859967&partnerID=8YFLogxK

U2 - 10.1093/femsle/fnx056

DO - 10.1093/femsle/fnx056

M3 - Article

C2 - 28333282

AN - SCOPUS:85019859967

VL - 364

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 6

M1 - fnx056

ER -