Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer

Takuma Fujii; Miyuki Saito; Takashi Iwata; Nobumaru Hirao; Hiroshi Nishio; Akiko Ohno; Katsumi Tsukazaki; Makio Mukai; Kaori Kameyama; Daisuke Aoki

doi:10.1007/s00428-008-0687-5

Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer

Takuma Fujii, Miyuki Saito, Takashi Iwata, Nobumaru Hirao, Hiroshi Nishio, Akiko Ohno, Katsumi Tsukazaki, Makio Mukai, Kaori Kameyama, Daisuke Aoki

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.

Original language	English
Pages (from-to)	545-555
Number of pages	11
Journal	Virchows Archiv
Volume	453
Issue number	6
DOIs	https://doi.org/10.1007/s00428-008-0687-5
Publication status	Published - 2008 Dec

Keywords

Cervical cancer screening
Human papillomavirus
In situ hybridization
Integration
Liquid-based cytology
P16

ASJC Scopus subject areas

Pathology and Forensic Medicine
Molecular Biology
Cell Biology

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Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer. / Fujii, Takuma; Saito, Miyuki; Iwata, Takashi; Hirao, Nobumaru; Nishio, Hiroshi; Ohno, Akiko; Tsukazaki, Katsumi; Mukai, Makio; Kameyama, Kaori; Aoki, Daisuke.

In: Virchows Archiv, Vol. 453, No. 6, 12.2008, p. 545-555.

Research output: Contribution to journal › Article › peer-review

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title = "Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer",

abstract = "Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.",

keywords = "Cervical cancer screening, Human papillomavirus, In situ hybridization, Integration, Liquid-based cytology, P16",

author = "Takuma Fujii and Miyuki Saito and Takashi Iwata and Nobumaru Hirao and Hiroshi Nishio and Akiko Ohno and Katsumi Tsukazaki and Makio Mukai and Kaori Kameyama and Daisuke Aoki",

note = "Funding Information: Acknowledgment This study was supported by a Grant-in-Aid for Scientific Research (C) Japan Society for the Promotion of Science and Research Grants for Life Science and Medicine. We thank Ms. Yuki Kamihara from Dako Japan Inc. and Dr. Katsuaki Dan from Keio University Central Research Laboratory for their technical assistance. We are grateful to Dr. Mattias Durst for the gift of the PK114/K plasmid containing the variant HPV 16 genome. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

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AU - Ohno, Akiko

AU - Tsukazaki, Katsumi

AU - Mukai, Makio

AU - Kameyama, Kaori

AU - Aoki, Daisuke

N1 - Funding Information: Acknowledgment This study was supported by a Grant-in-Aid for Scientific Research (C) Japan Society for the Promotion of Science and Research Grants for Life Science and Medicine. We thank Ms. Yuki Kamihara from Dako Japan Inc. and Dr. Katsuaki Dan from Keio University Central Research Laboratory for their technical assistance. We are grateful to Dr. Mattias Durst for the gift of the PK114/K plasmid containing the variant HPV 16 genome. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

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N2 - Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.

AB - Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.

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Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer

Abstract

Keywords

ASJC Scopus subject areas

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