TY - JOUR
T1 - Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer
AU - Fujii, Takuma
AU - Saito, Miyuki
AU - Iwata, Takashi
AU - Hirao, Nobumaru
AU - Nishio, Hiroshi
AU - Ohno, Akiko
AU - Tsukazaki, Katsumi
AU - Mukai, Makio
AU - Kameyama, Kaori
AU - Aoki, Daisuke
N1 - Funding Information:
Acknowledgment This study was supported by a Grant-in-Aid for Scientific Research (C) Japan Society for the Promotion of Science and Research Grants for Life Science and Medicine. We thank Ms. Yuki Kamihara from Dako Japan Inc. and Dr. Katsuaki Dan from Keio University Central Research Laboratory for their technical assistance. We are grateful to Dr. Mattias Durst for the gift of the PK114/K plasmid containing the variant HPV 16 genome.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2008/12
Y1 - 2008/12
N2 - Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.
AB - Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.
KW - Cervical cancer screening
KW - Human papillomavirus
KW - In situ hybridization
KW - Integration
KW - Liquid-based cytology
KW - P16
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U2 - 10.1007/s00428-008-0687-5
DO - 10.1007/s00428-008-0687-5
M3 - Article
C2 - 18936966
AN - SCOPUS:55849146643
VL - 453
SP - 545
EP - 555
JO - Virchows Archiv - Abteilung A Pathologische Anatomie
JF - Virchows Archiv - Abteilung A Pathologische Anatomie
SN - 0945-6317
IS - 6
ER -
