Abstract
Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 545-555 |
| Number of pages | 11 |
| Journal | Virchows Archiv |
| Volume | 453 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2008 Dec |
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Keywords
- Cervical cancer screening
- Human papillomavirus
- In situ hybridization
- Integration
- Liquid-based cytology
- P16
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Cell Biology
- Molecular Biology
Cite this
Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer. / Fujii, Takuma; Saito, Miyuki; Iwata, Takashi; Hirao, Nobumaru; Nishio, Hiroshi; Ohno, Akiko; Tsukazaki, Katsumi; Mukai, Makio; Kameyama, Kaori; Aoki, Daisuke.
In: Virchows Archiv, Vol. 453, No. 6, 12.2008, p. 545-555.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer
AU - Fujii, Takuma
AU - Saito, Miyuki
AU - Iwata, Takashi
AU - Hirao, Nobumaru
AU - Nishio, Hiroshi
AU - Ohno, Akiko
AU - Tsukazaki, Katsumi
AU - Mukai, Makio
AU - Kameyama, Kaori
AU - Aoki, Daisuke
PY - 2008/12
Y1 - 2008/12
N2 - Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.
AB - Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.
KW - Cervical cancer screening
KW - Human papillomavirus
KW - In situ hybridization
KW - Integration
KW - Liquid-based cytology
KW - P16
UR - http://www.scopus.com/inward/record.url?scp=55849146643&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=55849146643&partnerID=8YFLogxK
U2 - 10.1007/s00428-008-0687-5
DO - 10.1007/s00428-008-0687-5
M3 - Article
C2 - 18936966
AN - SCOPUS:55849146643
VL - 453
SP - 545
EP - 555
JO - Virchows Archiv
JF - Virchows Archiv
SN - 0945-6317
IS - 6
ER -