Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer

Takuma Fujii, Miyuki Saito, Takashi Iwata, Nobumaru Hirao, Hiroshi Nishio, Akiko Ohno, Katsumi Tsukazaki, Makio Mukai, Kaori Kameyama, Daisuke Aoki

Research output: Contribution to journalArticle

4 Citations (Scopus)


Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.

Original languageEnglish
Pages (from-to)545-555
Number of pages11
JournalVirchows Archiv
Issue number6
Publication statusPublished - 2008 Dec 1



  • Cervical cancer screening
  • Human papillomavirus
  • In situ hybridization
  • Integration
  • Liquid-based cytology
  • P16

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this