Angiopoietin-1 promotes lymphatic sprouting and hyperplasia

Tuomas Tammela, Anne Saaristo, Marja Lohela, Toru Morisada, Jenny Tornberg, Camilla Norrmén, Yuichi Oike, Katri Pajusola, Gavin Thurston, Toshio Suda, Seppo Yla-Herttuala, Kari Alitalo

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

Angiopoietin 1 (Ang1), a ligand for the receptor tyrosine kinase Tie2, regulates the formation and stabilization of the blood vessel network during embryogenesis. In adults, Ang1 is associated with blood vessel stabilization and recruitment of perivascular cells, whereas Ang2 acts to counter these actions. Recent results from gene-targeted mice have shown that Ang2 is also essential for the proper patterning of lymphatic vessels and that Ang1 can be substituted for this function. In order to characterize the effects of the angiopoietins on lymphatic vessels, we employed viral vectors for overexpression of Ang1 in adult mouse tissues. We found that Ang1 activated lymphatic vessel endothelial proliferation, vessel enlargement, and generation of long endothelial cell filopodia that eventually fused, leading to new sprouts and vessel development. Cutaneous lymphatic hyperplasia was also detected in transgenic mice expressing Ang1 in the basal epidermal cells. Tie2 was expressed in the lymphatic endothelial cells and Ang1 stimulation of these cells resulted in up-regulation of vascular endothelial growth factor receptor 3 (VEGFR-3). Furthermore, a soluble form of VEGFR-3 inhibited the observed lymphatic sprouting. Our results reinforce the concept that Ang1 therapy may be useful in settings of tissue edema.

Original languageEnglish
Pages (from-to)4642-4648
Number of pages7
JournalBlood
Volume105
Issue number12
DOIs
Publication statusPublished - 2005 Jun 15
Externally publishedYes

Fingerprint

Angiopoietin-1
Hyperplasia
Lymphatic Vessels
Vascular Endothelial Growth Factor Receptor-3
Endothelial cells
Blood vessels
Blood Vessels
Endothelial Cells
Stabilization
Angiopoietins
Tissue
Pseudopodia
Receptor Protein-Tyrosine Kinases
Transgenic Mice
Embryonic Development
Edema
Up-Regulation
Genes
Ligands
Skin

ASJC Scopus subject areas

  • Hematology

Cite this

Tammela, T., Saaristo, A., Lohela, M., Morisada, T., Tornberg, J., Norrmén, C., ... Alitalo, K. (2005). Angiopoietin-1 promotes lymphatic sprouting and hyperplasia. Blood, 105(12), 4642-4648. https://doi.org/10.1182/blood-2004-08-3327

Angiopoietin-1 promotes lymphatic sprouting and hyperplasia. / Tammela, Tuomas; Saaristo, Anne; Lohela, Marja; Morisada, Toru; Tornberg, Jenny; Norrmén, Camilla; Oike, Yuichi; Pajusola, Katri; Thurston, Gavin; Suda, Toshio; Yla-Herttuala, Seppo; Alitalo, Kari.

In: Blood, Vol. 105, No. 12, 15.06.2005, p. 4642-4648.

Research output: Contribution to journalArticle

Tammela, T, Saaristo, A, Lohela, M, Morisada, T, Tornberg, J, Norrmén, C, Oike, Y, Pajusola, K, Thurston, G, Suda, T, Yla-Herttuala, S & Alitalo, K 2005, 'Angiopoietin-1 promotes lymphatic sprouting and hyperplasia', Blood, vol. 105, no. 12, pp. 4642-4648. https://doi.org/10.1182/blood-2004-08-3327
Tammela T, Saaristo A, Lohela M, Morisada T, Tornberg J, Norrmén C et al. Angiopoietin-1 promotes lymphatic sprouting and hyperplasia. Blood. 2005 Jun 15;105(12):4642-4648. https://doi.org/10.1182/blood-2004-08-3327
Tammela, Tuomas ; Saaristo, Anne ; Lohela, Marja ; Morisada, Toru ; Tornberg, Jenny ; Norrmén, Camilla ; Oike, Yuichi ; Pajusola, Katri ; Thurston, Gavin ; Suda, Toshio ; Yla-Herttuala, Seppo ; Alitalo, Kari. / Angiopoietin-1 promotes lymphatic sprouting and hyperplasia. In: Blood. 2005 ; Vol. 105, No. 12. pp. 4642-4648.
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