Angiopoietin-2-driven vascular remodeling in airway inflammation

Sebastien P. Tabruyn, Katharine Colton, Toru Morisada, Jonas Fuxe, Stanley J. Wiegand, Gavin Thurston, Anthony J. Coyle, Jane Connor, Donald M. McDonald

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Vascular remodeling is a feature of chronic inflammation during which capillaries transform into venules that expand the region of the vasculature in which leakage and leukocyte emigration both occur. Recently, we found that angiopoietin/Tie2 receptor signaling drives the transformation of capillaries into venules at an early stage of the sustained inflammatory response in the airways of mice infected with Mycoplasma pulmonis. However, the precise contributions of both angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) are not clear. In this study, we sought to determine the contribution of Ang2 to this vascular remodeling. Ang2 mRNA expression levels increased and phosphorylated Tie2 immunoreactivity in mucosal blood vessels decreased, indicative of diminished receptor signaling after infection. Selective inhibition of Ang2 throughout the infection by administration of either of two distinct function-blocking antibodies reduced the suppression of Tie2 phosphorylation and decreased the remodeling of mucosal capillaries into venules, the amount of leukocyte influx, and disease severity. These findings are consistent with Ang2 acting as an antagonist of Tie2 receptors and the reduction of Tie2 phosphorylation in endothelial cells rendering the vasculature more responsive to cytokines that promote both vascular remodeling and the consequences of inflammation after M. pulmonis infection. By blocking such changes, Ang2 inhibitors may prove beneficial in the treatment of sustained inflammation in which vascular remodeling, leakage, and leukocyte influx contribute to its pathophysiology.

Original languageEnglish
Pages (from-to)3233-3244
Number of pages12
JournalAmerican Journal of Pathology
Volume177
Issue number6
DOIs
Publication statusPublished - 2010 Dec
Externally publishedYes

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Angiopoietin-2
Inflammation
Venules
TIE-2 Receptor
Mycoplasma pulmonis
Leukocytes
Phosphorylation
Angiopoietins
Angiopoietin-1
Mycoplasma Infections
Blocking Antibodies
Emigration and Immigration
Infection
Blood Vessels
Vascular Remodeling
Endothelial Cells
Cytokines
Messenger RNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Tabruyn, S. P., Colton, K., Morisada, T., Fuxe, J., Wiegand, S. J., Thurston, G., ... McDonald, D. M. (2010). Angiopoietin-2-driven vascular remodeling in airway inflammation. American Journal of Pathology, 177(6), 3233-3244. https://doi.org/10.2353/ajpath.2010.100059

Angiopoietin-2-driven vascular remodeling in airway inflammation. / Tabruyn, Sebastien P.; Colton, Katharine; Morisada, Toru; Fuxe, Jonas; Wiegand, Stanley J.; Thurston, Gavin; Coyle, Anthony J.; Connor, Jane; McDonald, Donald M.

In: American Journal of Pathology, Vol. 177, No. 6, 12.2010, p. 3233-3244.

Research output: Contribution to journalArticle

Tabruyn, SP, Colton, K, Morisada, T, Fuxe, J, Wiegand, SJ, Thurston, G, Coyle, AJ, Connor, J & McDonald, DM 2010, 'Angiopoietin-2-driven vascular remodeling in airway inflammation', American Journal of Pathology, vol. 177, no. 6, pp. 3233-3244. https://doi.org/10.2353/ajpath.2010.100059
Tabruyn, Sebastien P. ; Colton, Katharine ; Morisada, Toru ; Fuxe, Jonas ; Wiegand, Stanley J. ; Thurston, Gavin ; Coyle, Anthony J. ; Connor, Jane ; McDonald, Donald M. / Angiopoietin-2-driven vascular remodeling in airway inflammation. In: American Journal of Pathology. 2010 ; Vol. 177, No. 6. pp. 3233-3244.
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