Anion-exchange blocker enhances cytoplasmic vacuole formation and cell death in serum-deprived mouse kidney epithelial cells in mice

Takashi Araki, Matsuhiko Hayashi, Takao Saruta

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

An anion exchange blocker, DIDS, exhibits anti-apoptotic activity in response to several apoptotic stimuli, but has an opposite effect when apoptosis is induced by serum deprivation. After adding DIDS, serum-deprived MCT cells exhibited vacuole formation in their cytoplasm and underwent cell death. Caspase activity increased with the addition of DIDS to serum-deprived MCT cells, but Z-Asp-CH2-DCB, a caspase inhibitor, did not inhibit cell death in DIDS-treated, serum-deprived MCT cells. Cathepsin is considered to be important for vacuole formation and cell lysis, and pepstatin A, a cathepsin D inhibitor, partially inhibited vacuole formation in DIDS-treated, serum-deprived MCT cells, although caspase activation was not inhibited. 3-Methyladenin inhibited vacuole formation and cell death in DIDS-treated, serum-deprived MCT cells. These results suggest that DIDS-treated serum-deprived MCT cells undergo autophagy, not apoptosis.

Original languageEnglish
Pages (from-to)93-100
Number of pages8
JournalCell Biology International
Volume30
Issue number1
DOIs
Publication statusPublished - 2006 Jan

Fingerprint

4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Vacuoles
Anions
Cell Death
Epithelial Cells
Kidney
Serum
Caspases
Apoptosis
Cathepsins
Cathepsin D
Caspase Inhibitors
Autophagy
Cytoplasm

Keywords

  • 3-MA
  • Apoptosis
  • Autophagy
  • Cathepsin
  • DIDS

ASJC Scopus subject areas

  • Cell Biology

Cite this

Anion-exchange blocker enhances cytoplasmic vacuole formation and cell death in serum-deprived mouse kidney epithelial cells in mice. / Araki, Takashi; Hayashi, Matsuhiko; Saruta, Takao.

In: Cell Biology International, Vol. 30, No. 1, 01.2006, p. 93-100.

Research output: Contribution to journalArticle

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