Abstract
An anion exchange blocker, DIDS, exhibits anti-apoptotic activity in response to several apoptotic stimuli, but has an opposite effect when apoptosis is induced by serum deprivation. After adding DIDS, serum-deprived MCT cells exhibited vacuole formation in their cytoplasm and underwent cell death. Caspase activity increased with the addition of DIDS to serum-deprived MCT cells, but Z-Asp-CH2-DCB, a caspase inhibitor, did not inhibit cell death in DIDS-treated, serum-deprived MCT cells. Cathepsin is considered to be important for vacuole formation and cell lysis, and pepstatin A, a cathepsin D inhibitor, partially inhibited vacuole formation in DIDS-treated, serum-deprived MCT cells, although caspase activation was not inhibited. 3-Methyladenin inhibited vacuole formation and cell death in DIDS-treated, serum-deprived MCT cells. These results suggest that DIDS-treated serum-deprived MCT cells undergo autophagy, not apoptosis.
| Original language | English |
|---|---|
| Pages (from-to) | 93-100 |
| Number of pages | 8 |
| Journal | Cell Biology International |
| Volume | 30 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2006 Jan |
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Keywords
- 3-MA
- Apoptosis
- Autophagy
- Cathepsin
- DIDS
ASJC Scopus subject areas
- Cell Biology
Cite this
Anion-exchange blocker enhances cytoplasmic vacuole formation and cell death in serum-deprived mouse kidney epithelial cells in mice. / Araki, Takashi; Hayashi, Matsuhiko; Saruta, Takao.
In: Cell Biology International, Vol. 30, No. 1, 01.2006, p. 93-100.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anion-exchange blocker enhances cytoplasmic vacuole formation and cell death in serum-deprived mouse kidney epithelial cells in mice
AU - Araki, Takashi
AU - Hayashi, Matsuhiko
AU - Saruta, Takao
PY - 2006/1
Y1 - 2006/1
N2 - An anion exchange blocker, DIDS, exhibits anti-apoptotic activity in response to several apoptotic stimuli, but has an opposite effect when apoptosis is induced by serum deprivation. After adding DIDS, serum-deprived MCT cells exhibited vacuole formation in their cytoplasm and underwent cell death. Caspase activity increased with the addition of DIDS to serum-deprived MCT cells, but Z-Asp-CH2-DCB, a caspase inhibitor, did not inhibit cell death in DIDS-treated, serum-deprived MCT cells. Cathepsin is considered to be important for vacuole formation and cell lysis, and pepstatin A, a cathepsin D inhibitor, partially inhibited vacuole formation in DIDS-treated, serum-deprived MCT cells, although caspase activation was not inhibited. 3-Methyladenin inhibited vacuole formation and cell death in DIDS-treated, serum-deprived MCT cells. These results suggest that DIDS-treated serum-deprived MCT cells undergo autophagy, not apoptosis.
AB - An anion exchange blocker, DIDS, exhibits anti-apoptotic activity in response to several apoptotic stimuli, but has an opposite effect when apoptosis is induced by serum deprivation. After adding DIDS, serum-deprived MCT cells exhibited vacuole formation in their cytoplasm and underwent cell death. Caspase activity increased with the addition of DIDS to serum-deprived MCT cells, but Z-Asp-CH2-DCB, a caspase inhibitor, did not inhibit cell death in DIDS-treated, serum-deprived MCT cells. Cathepsin is considered to be important for vacuole formation and cell lysis, and pepstatin A, a cathepsin D inhibitor, partially inhibited vacuole formation in DIDS-treated, serum-deprived MCT cells, although caspase activation was not inhibited. 3-Methyladenin inhibited vacuole formation and cell death in DIDS-treated, serum-deprived MCT cells. These results suggest that DIDS-treated serum-deprived MCT cells undergo autophagy, not apoptosis.
KW - 3-MA
KW - Apoptosis
KW - Autophagy
KW - Cathepsin
KW - DIDS
UR - http://www.scopus.com/inward/record.url?scp=32844473214&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=32844473214&partnerID=8YFLogxK
U2 - 10.1016/j.cellbi.2005.10.020
DO - 10.1016/j.cellbi.2005.10.020
M3 - Article
C2 - 16376114
AN - SCOPUS:32844473214
VL - 30
SP - 93
EP - 100
JO - Cell Biology International
JF - Cell Biology International
SN - 1065-6995
IS - 1
ER -