Anion-exchange blocker enhances cytoplasmic vacuole formation and cell death in serum-deprived mouse kidney epithelial cells in mice

Takashi Araki, Matsuhiko Hayashi, Takao Saruta

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

An anion exchange blocker, DIDS, exhibits anti-apoptotic activity in response to several apoptotic stimuli, but has an opposite effect when apoptosis is induced by serum deprivation. After adding DIDS, serum-deprived MCT cells exhibited vacuole formation in their cytoplasm and underwent cell death. Caspase activity increased with the addition of DIDS to serum-deprived MCT cells, but Z-Asp-CH2-DCB, a caspase inhibitor, did not inhibit cell death in DIDS-treated, serum-deprived MCT cells. Cathepsin is considered to be important for vacuole formation and cell lysis, and pepstatin A, a cathepsin D inhibitor, partially inhibited vacuole formation in DIDS-treated, serum-deprived MCT cells, although caspase activation was not inhibited. 3-Methyladenin inhibited vacuole formation and cell death in DIDS-treated, serum-deprived MCT cells. These results suggest that DIDS-treated serum-deprived MCT cells undergo autophagy, not apoptosis.

Original languageEnglish
Pages (from-to)93-100
Number of pages8
JournalCell Biology International
Volume30
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1

Keywords

  • 3-MA
  • Apoptosis
  • Autophagy
  • Cathepsin
  • DIDS

ASJC Scopus subject areas

  • Cell Biology

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