TY - JOUR
T1 - Anion-exchange blocker enhances cytoplasmic vacuole formation and cell death in serum-deprived mouse kidney epithelial cells in mice
AU - Araki, Takashi
AU - Hayashi, Matsuhiko
AU - Saruta, Takao
N1 - Funding Information:
We thank Dr Masayuki Miura for suggestions. This research was supported in part by grants from the Ministry of Education, Science and Culture in Japan and from the Naito Foundation to T.A, and from Health Science Research Grants from the Ministry of Health and Welfare to M.H.
PY - 2006/1
Y1 - 2006/1
N2 - An anion exchange blocker, DIDS, exhibits anti-apoptotic activity in response to several apoptotic stimuli, but has an opposite effect when apoptosis is induced by serum deprivation. After adding DIDS, serum-deprived MCT cells exhibited vacuole formation in their cytoplasm and underwent cell death. Caspase activity increased with the addition of DIDS to serum-deprived MCT cells, but Z-Asp-CH2-DCB, a caspase inhibitor, did not inhibit cell death in DIDS-treated, serum-deprived MCT cells. Cathepsin is considered to be important for vacuole formation and cell lysis, and pepstatin A, a cathepsin D inhibitor, partially inhibited vacuole formation in DIDS-treated, serum-deprived MCT cells, although caspase activation was not inhibited. 3-Methyladenin inhibited vacuole formation and cell death in DIDS-treated, serum-deprived MCT cells. These results suggest that DIDS-treated serum-deprived MCT cells undergo autophagy, not apoptosis.
AB - An anion exchange blocker, DIDS, exhibits anti-apoptotic activity in response to several apoptotic stimuli, but has an opposite effect when apoptosis is induced by serum deprivation. After adding DIDS, serum-deprived MCT cells exhibited vacuole formation in their cytoplasm and underwent cell death. Caspase activity increased with the addition of DIDS to serum-deprived MCT cells, but Z-Asp-CH2-DCB, a caspase inhibitor, did not inhibit cell death in DIDS-treated, serum-deprived MCT cells. Cathepsin is considered to be important for vacuole formation and cell lysis, and pepstatin A, a cathepsin D inhibitor, partially inhibited vacuole formation in DIDS-treated, serum-deprived MCT cells, although caspase activation was not inhibited. 3-Methyladenin inhibited vacuole formation and cell death in DIDS-treated, serum-deprived MCT cells. These results suggest that DIDS-treated serum-deprived MCT cells undergo autophagy, not apoptosis.
KW - 3-MA
KW - Apoptosis
KW - Autophagy
KW - Cathepsin
KW - DIDS
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U2 - 10.1016/j.cellbi.2005.10.020
DO - 10.1016/j.cellbi.2005.10.020
M3 - Article
C2 - 16376114
AN - SCOPUS:32844473214
VL - 30
SP - 93
EP - 100
JO - Cell Biology International Reports
JF - Cell Biology International Reports
SN - 1065-6995
IS - 1
ER -