Abstract
This paper describes anisotropic spherical collagen hydrogel microparticles for confined 3D cell culture system for the first time. We fabricated uniform-sized collagen microparticles simply by ejecting micro-droplets containing collagen pre-gel solution and sodium alginate into calcium chloride solution under high centrifugal gravity: we used calcium alginate hydrogel as a «sacrificial molecular template» for the gelation of pure collagen microparticles. Using our collagen microparticles, we successfully demonstrated in encapsulating differently labeled cells in each hemisphere at high viability and in culturing the cells inside the microparticles. Our anisotropic collagen microparticles could be powerful tools to co-culture multiple cells in different ECMs and to control differentiation of stem cells in an anisotropic microenvironment at single-particles with massive and quantitative analyses.
| Original language | English |
|---|---|
| Title of host publication | MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| Pages | 263-266 |
| Number of pages | 4 |
| Volume | 2016-February |
| ISBN (Electronic) | 9781509019731 |
| DOIs | |
| Publication status | Published - 2016 Feb 26 |
| Event | 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China Duration: 2016 Jan 24 → 2016 Jan 28 |
Other
| Other | 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 |
|---|---|
| Country/Territory | China |
| City | Shanghai |
| Period | 16/1/24 → 16/1/28 |
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Mechanical Engineering
- Condensed Matter Physics
- Electronic, Optical and Magnetic Materials