Anisotropic spherical collagen microparticles for confined 3D cell culture system with spatially designed microenvironment

Satoru Yoshida, Masahiro Takinoue, Hiroaki Onoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This paper describes anisotropic spherical collagen hydrogel microparticles for confined 3D cell culture system for the first time. We fabricated uniform-sized collagen microparticles simply by ejecting micro-droplets containing collagen pre-gel solution and sodium alginate into calcium chloride solution under high centrifugal gravity: we used calcium alginate hydrogel as a «sacrificial molecular template» for the gelation of pure collagen microparticles. Using our collagen microparticles, we successfully demonstrated in encapsulating differently labeled cells in each hemisphere at high viability and in culturing the cells inside the microparticles. Our anisotropic collagen microparticles could be powerful tools to co-culture multiple cells in different ECMs and to control differentiation of stem cells in an anisotropic microenvironment at single-particles with massive and quantitative analyses.

Original languageEnglish
Title of host publicationMEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages263-266
Number of pages4
Volume2016-February
ISBN (Electronic)9781509019731
DOIs
Publication statusPublished - 2016 Feb 26
Event29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China
Duration: 2016 Jan 242016 Jan 28

Other

Other29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
CountryChina
CityShanghai
Period16/1/2416/1/28

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ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Yoshida, S., Takinoue, M., & Onoe, H. (2016). Anisotropic spherical collagen microparticles for confined 3D cell culture system with spatially designed microenvironment. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems (Vol. 2016-February, pp. 263-266). [7421610] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2016.7421610