Anodic stripping voltammetry of gold nanoparticles at boron-doped diamond electrodes and its application in immunochromatographic strip tests

Tribidasari A. Ivandini, Wiyogo P. Wicaksono, Endang Saepudin, Bakhadir Rismetov, Yasuaki Einaga

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Anodic stripping voltammetry (ASV) of colloidal gold-nanoparticles (AuNPs) was investigated at boron-doped diamond (BDD) electrodes in 50 mM HClO4. A deposition time of 300 s at-0.2 V (vs. Ag/AgCl) was fixed as the condition for the ASV. The voltammograms showed oxidation peaks that could be attributed to the oxidation of gold. These oxidation peaks were then investigated for potential application in immunochromatographic strip tests for the selective and quantitative detection of melamine, in which AuNPs were used as the label for the antibody of melamine. Linear regression of the oxidation peak currents appeared in the concentration range from 0.05-0.6 μg/mL melamine standard, with an estimated LOD of 0.069 μg/mL and an average relative standard deviation of 8.0%. This indicated that the method could be considered as an alternative method for selective and quantitative immunochromatographic applications. The validity was examined by the measurements of melamine injected into milk samples, which showed good recovery percentages during the measurements.

Original languageEnglish
Pages (from-to)136-143
Number of pages8
JournalTalanta
Volume134
DOIs
Publication statusPublished - 2015 Mar 1

Keywords

  • Anodic stripping voltammetry
  • Boron-doped diamond electrode
  • Gold nanoparticles
  • Immunochromatographic strip tests
  • Melamine

ASJC Scopus subject areas

  • Analytical Chemistry

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