Anti-asialo GM1 antibody suppression of cyclophosphamide-induced diabetes in NOD mice

T. Maruyama, Kenji Watanabe, I. Takei, A. Kasuga, A. Shimada, T. Yanagawa, T. Kasatani, Y. Suzuki, K. Kataoka, T. Saruta, S. Habu

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

To elucidate the role of natural killer (NK) cells in the pathogenesis of diabetes in the non-obese diabetic (NOD) mouse, we examined whether or not cyclophosphamide-induced diabetes occurs in NOD mice intraperitoneally (i.p.) injected with anti-asialo GM1 antibody. Two weeks after a single intraperitoneal injection of cyclophosphamide, none of the 24 NOD mice which had previously been treated with anti-asialo GM1 antibody, 2-3 times per week for either 2 or 3 weeks, had developed indications of diabetes such as glycosuria or a high plasma glucose level. On the other hand, signs of diabetes were found in 10 of 24 control NOD mice injected with normal rabbit Ig instead of anti-asialo GM1 antibody (p<0.01). The NK cell activities of spleen cells from anti-asialo GM1 antibody-treated mice were significantly lower than those of control mice (p<0.01). Flowcytometry analysis demonstrated that anti-asialo GM1 antibody-positive cells had disappeared from the spleens of anti-asialo GM1 antibody-injected mice but no suppression of CD8+ and CD4+ cells could be demonstrated. These observations suggest that NK cells are involved in the development of diabetes in NOD mice.

Original languageEnglish
Pages (from-to)37-41
Number of pages5
JournalDiabetes Research
Volume17
Issue number1
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Inbred NOD Mouse
Cyclophosphamide
Antibodies
Natural Killer Cells
Spleen
Glycosuria
Intraperitoneal Injections
asialo GM1 ganglioside
Rabbits
Glucose

Keywords

  • Anti-asialo GM1 antibody
  • Cyclophosphamide
  • NK-cell
  • NOD mouse
  • Prevention

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Maruyama, T., Watanabe, K., Takei, I., Kasuga, A., Shimada, A., Yanagawa, T., ... Habu, S. (1991). Anti-asialo GM1 antibody suppression of cyclophosphamide-induced diabetes in NOD mice. Diabetes Research, 17(1), 37-41.

Anti-asialo GM1 antibody suppression of cyclophosphamide-induced diabetes in NOD mice. / Maruyama, T.; Watanabe, Kenji; Takei, I.; Kasuga, A.; Shimada, A.; Yanagawa, T.; Kasatani, T.; Suzuki, Y.; Kataoka, K.; Saruta, T.; Habu, S.

In: Diabetes Research, Vol. 17, No. 1, 1991, p. 37-41.

Research output: Contribution to journalArticle

Maruyama, T, Watanabe, K, Takei, I, Kasuga, A, Shimada, A, Yanagawa, T, Kasatani, T, Suzuki, Y, Kataoka, K, Saruta, T & Habu, S 1991, 'Anti-asialo GM1 antibody suppression of cyclophosphamide-induced diabetes in NOD mice', Diabetes Research, vol. 17, no. 1, pp. 37-41.
Maruyama T, Watanabe K, Takei I, Kasuga A, Shimada A, Yanagawa T et al. Anti-asialo GM1 antibody suppression of cyclophosphamide-induced diabetes in NOD mice. Diabetes Research. 1991;17(1):37-41.
Maruyama, T. ; Watanabe, Kenji ; Takei, I. ; Kasuga, A. ; Shimada, A. ; Yanagawa, T. ; Kasatani, T. ; Suzuki, Y. ; Kataoka, K. ; Saruta, T. ; Habu, S. / Anti-asialo GM1 antibody suppression of cyclophosphamide-induced diabetes in NOD mice. In: Diabetes Research. 1991 ; Vol. 17, No. 1. pp. 37-41.
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