Anti-desmoglein IgG autoantibodies in patients with pemphigus in remission

E. J. Kwon, Jun Yamagami, T. Nishikawa, Masayuki Amagai

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Background: Desmoglein (Dsg) enzyme-linked immunosorbent assay (ELISA) is a highly sensitive and specific method to detect anti-Dsg3 and anti-Dsg1 IgG autoantibodies in pemphigus vulgaris (PV) and pemphigus foliaceus (PF), respectively. Whereas ELISA index values fluctuate in parallel with disease activity, ELISA positivity during clinical remission has been observed. Objective: To determine the prevalence of positive Dsg ELISA index values during clinical remission. To ascertain how positive Dsg ELISA scores during remission compare with those during active disease. Methods: Dsg ELISA was performed on serum samples of PV and PF patients taken during remission (lesion-free ≥ 3 months on ≤ 15 mg or ≤ 5 mg/day prednisolone) and active disease. We used a modified ELISA protocol with optimal serum dilutions in sera with very high initial index values, as we previously described. Results: When remission was defined as no eruption ≥ 3 months with ≤ 15 mg/day prednisolone, 20 of 43 PV patients (46.5%) and 4 of 12 PF patients (33.3%) showed Dsg3 and Dsg1 ELISA positivity, respectively. With ≤ 5 mg/day, 6 of 17 PV (35.3%) and 1 of 6 PF patients (16.7%) showed Dsg3 and Dsg1 ELISA positivity, respectively. The index value of each ELISA-positive remission serum was consistently lower than that of its corresponding active disease serum. We observed consistent correlation between ELISA index values and indirect immunofluorescence titres. Conclusions: Circulating anti-Dsg IgG autoantibodies are found in a considerable percentage of pemphigus patients in remission, who have high levels of antibody production during active stages.

Original languageEnglish
Pages (from-to)1070-1075
Number of pages6
JournalJournal of the European Academy of Dermatology and Venereology
Volume22
Issue number9
DOIs
Publication statusPublished - 2008 Sep

Fingerprint

Desmogleins
Pemphigus
Autoantibodies
Enzyme-Linked Immunosorbent Assay
Serum
Prednisolone
anti-IgG
Indirect Fluorescent Antibody Technique
Antibody Formation

Keywords

  • Desmoglein enzyme-linked immunosorbent assay
  • Indirect immunofluorescence
  • Pemphigus

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

Cite this

Anti-desmoglein IgG autoantibodies in patients with pemphigus in remission. / Kwon, E. J.; Yamagami, Jun; Nishikawa, T.; Amagai, Masayuki.

In: Journal of the European Academy of Dermatology and Venereology, Vol. 22, No. 9, 09.2008, p. 1070-1075.

Research output: Contribution to journalArticle

@article{9c8fbe42f7ff42ada70a1f23bc8712c2,
title = "Anti-desmoglein IgG autoantibodies in patients with pemphigus in remission",
abstract = "Background: Desmoglein (Dsg) enzyme-linked immunosorbent assay (ELISA) is a highly sensitive and specific method to detect anti-Dsg3 and anti-Dsg1 IgG autoantibodies in pemphigus vulgaris (PV) and pemphigus foliaceus (PF), respectively. Whereas ELISA index values fluctuate in parallel with disease activity, ELISA positivity during clinical remission has been observed. Objective: To determine the prevalence of positive Dsg ELISA index values during clinical remission. To ascertain how positive Dsg ELISA scores during remission compare with those during active disease. Methods: Dsg ELISA was performed on serum samples of PV and PF patients taken during remission (lesion-free ≥ 3 months on ≤ 15 mg or ≤ 5 mg/day prednisolone) and active disease. We used a modified ELISA protocol with optimal serum dilutions in sera with very high initial index values, as we previously described. Results: When remission was defined as no eruption ≥ 3 months with ≤ 15 mg/day prednisolone, 20 of 43 PV patients (46.5{\%}) and 4 of 12 PF patients (33.3{\%}) showed Dsg3 and Dsg1 ELISA positivity, respectively. With ≤ 5 mg/day, 6 of 17 PV (35.3{\%}) and 1 of 6 PF patients (16.7{\%}) showed Dsg3 and Dsg1 ELISA positivity, respectively. The index value of each ELISA-positive remission serum was consistently lower than that of its corresponding active disease serum. We observed consistent correlation between ELISA index values and indirect immunofluorescence titres. Conclusions: Circulating anti-Dsg IgG autoantibodies are found in a considerable percentage of pemphigus patients in remission, who have high levels of antibody production during active stages.",
keywords = "Desmoglein enzyme-linked immunosorbent assay, Indirect immunofluorescence, Pemphigus",
author = "Kwon, {E. J.} and Jun Yamagami and T. Nishikawa and Masayuki Amagai",
year = "2008",
month = "9",
doi = "10.1111/j.1468-3083.2008.02715.x",
language = "English",
volume = "22",
pages = "1070--1075",
journal = "Journal of the European Academy of Dermatology and Venereology",
issn = "0926-9959",
publisher = "Wiley-Blackwell",
number = "9",

}

TY - JOUR

T1 - Anti-desmoglein IgG autoantibodies in patients with pemphigus in remission

AU - Kwon, E. J.

AU - Yamagami, Jun

AU - Nishikawa, T.

AU - Amagai, Masayuki

PY - 2008/9

Y1 - 2008/9

N2 - Background: Desmoglein (Dsg) enzyme-linked immunosorbent assay (ELISA) is a highly sensitive and specific method to detect anti-Dsg3 and anti-Dsg1 IgG autoantibodies in pemphigus vulgaris (PV) and pemphigus foliaceus (PF), respectively. Whereas ELISA index values fluctuate in parallel with disease activity, ELISA positivity during clinical remission has been observed. Objective: To determine the prevalence of positive Dsg ELISA index values during clinical remission. To ascertain how positive Dsg ELISA scores during remission compare with those during active disease. Methods: Dsg ELISA was performed on serum samples of PV and PF patients taken during remission (lesion-free ≥ 3 months on ≤ 15 mg or ≤ 5 mg/day prednisolone) and active disease. We used a modified ELISA protocol with optimal serum dilutions in sera with very high initial index values, as we previously described. Results: When remission was defined as no eruption ≥ 3 months with ≤ 15 mg/day prednisolone, 20 of 43 PV patients (46.5%) and 4 of 12 PF patients (33.3%) showed Dsg3 and Dsg1 ELISA positivity, respectively. With ≤ 5 mg/day, 6 of 17 PV (35.3%) and 1 of 6 PF patients (16.7%) showed Dsg3 and Dsg1 ELISA positivity, respectively. The index value of each ELISA-positive remission serum was consistently lower than that of its corresponding active disease serum. We observed consistent correlation between ELISA index values and indirect immunofluorescence titres. Conclusions: Circulating anti-Dsg IgG autoantibodies are found in a considerable percentage of pemphigus patients in remission, who have high levels of antibody production during active stages.

AB - Background: Desmoglein (Dsg) enzyme-linked immunosorbent assay (ELISA) is a highly sensitive and specific method to detect anti-Dsg3 and anti-Dsg1 IgG autoantibodies in pemphigus vulgaris (PV) and pemphigus foliaceus (PF), respectively. Whereas ELISA index values fluctuate in parallel with disease activity, ELISA positivity during clinical remission has been observed. Objective: To determine the prevalence of positive Dsg ELISA index values during clinical remission. To ascertain how positive Dsg ELISA scores during remission compare with those during active disease. Methods: Dsg ELISA was performed on serum samples of PV and PF patients taken during remission (lesion-free ≥ 3 months on ≤ 15 mg or ≤ 5 mg/day prednisolone) and active disease. We used a modified ELISA protocol with optimal serum dilutions in sera with very high initial index values, as we previously described. Results: When remission was defined as no eruption ≥ 3 months with ≤ 15 mg/day prednisolone, 20 of 43 PV patients (46.5%) and 4 of 12 PF patients (33.3%) showed Dsg3 and Dsg1 ELISA positivity, respectively. With ≤ 5 mg/day, 6 of 17 PV (35.3%) and 1 of 6 PF patients (16.7%) showed Dsg3 and Dsg1 ELISA positivity, respectively. The index value of each ELISA-positive remission serum was consistently lower than that of its corresponding active disease serum. We observed consistent correlation between ELISA index values and indirect immunofluorescence titres. Conclusions: Circulating anti-Dsg IgG autoantibodies are found in a considerable percentage of pemphigus patients in remission, who have high levels of antibody production during active stages.

KW - Desmoglein enzyme-linked immunosorbent assay

KW - Indirect immunofluorescence

KW - Pemphigus

UR - http://www.scopus.com/inward/record.url?scp=49649088636&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49649088636&partnerID=8YFLogxK

U2 - 10.1111/j.1468-3083.2008.02715.x

DO - 10.1111/j.1468-3083.2008.02715.x

M3 - Article

VL - 22

SP - 1070

EP - 1075

JO - Journal of the European Academy of Dermatology and Venereology

JF - Journal of the European Academy of Dermatology and Venereology

SN - 0926-9959

IS - 9

ER -