Anti-high mobility group box 1 and box 2 non-histone chromosomal proteins (HMGB1/HMGB2) antibodies and anti-Saccharomyces cerevisiae antibodies (ASCA): Accuracy in differentially diagnosing UC and CD and correlation with inflammatory bowel disease phenotype

Hiromasa Takaishi, Takanori Kanai, Atsushi Nakazawa, Fumihiko Sugata, Akira Nikai, Shigeo Yoshizawa, Yasuo Hamamoto, Shinsuke Funakoshi, Tomoharu Yajima, Yasushi Iwao, Masao Takemura, Shoichi Ozaki, Toshifumi Hibi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background The development of a supportive diagnostic method has long been required to differentially diagnose ulcerative colitis (UC) and Crohn's disease (CD). Several antibodies circulate in the sera of patients with inflammatory bowel disease. We previously identified the high mobility group box 1 and box 2 non-histone chromosomal proteins (HMGB1 and HMGB2) as novel antigens of perinuclear type anti-neutrophil cytoplasmic antibodies (pANCA) and discovered anti-HMGB1/HMGB2 antibodies in sera from patients with UC. Here, we evaluated the ability of anti-HMGB1/HMGB2 antibodies combined with anti-Saccharomyces cerevisiae antibodies (ASCA) to differentially diagnose UC and CD. Methods We measured titers of anti-HMGB1/HMGB2 antibodies and ASCA in the sera of 213 patients with UC and 93 with CD, using enzyme-linked immunosorbent assays. Results Among the patients with UC, 26.8% were positive for anti-HMGB1/HMGB2 antibodies, with 85.0% specificity towards CD and a positive predictive value of 80.3%. Corticosteroids significantly suppressed the titer of anti- HMGB1/HMGB2 antibodies. Among the patients with CD, 24.7% were positive for ASCA, with 96.2% specificity towards UC and a positive predictive value of 74.2%. Interestingly, the positivity rate of anti-HMGB/HMGB2 antibodies was higher (35.7%) in patients with the ileitis type ofCDthan in patients withCDin the colon (6.2%; significant difference, P\0.01). The specificity of anti-HMGB1/ HMGB2 antibodies in UC for CD in the colon was 93.8%. Conclusions CD in the colon and UC can be differentially diagnosed using anti-HMGB/HMGB2 antibodies combined with ASCA.

Original languageEnglish
Pages (from-to)969-977
Number of pages9
JournalJournal of Gastroenterology
Volume47
Issue number9
DOIs
Publication statusPublished - 2012 Sep

Fingerprint

HMGB2 Protein
Non Histone Chromosomal Proteins
HMGB1 Protein
Ulcerative Colitis
Inflammatory Bowel Diseases
Crohn Disease
Saccharomyces cerevisiae
Anti-Idiotypic Antibodies
Phenotype
Antibodies
HMGB Proteins
Colon
Serum
Ileitis
Antineutrophil Cytoplasmic Antibodies

Keywords

  • Anti-high mobility group box 1 and box 2 non-histone chromosomal proteins
  • Anti-Saccharomyces cerevisiae antibodies
  • Crohn's disease
  • Ulcerative colitis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Anti-high mobility group box 1 and box 2 non-histone chromosomal proteins (HMGB1/HMGB2) antibodies and anti-Saccharomyces cerevisiae antibodies (ASCA) : Accuracy in differentially diagnosing UC and CD and correlation with inflammatory bowel disease phenotype. / Takaishi, Hiromasa; Kanai, Takanori; Nakazawa, Atsushi; Sugata, Fumihiko; Nikai, Akira; Yoshizawa, Shigeo; Hamamoto, Yasuo; Funakoshi, Shinsuke; Yajima, Tomoharu; Iwao, Yasushi; Takemura, Masao; Ozaki, Shoichi; Hibi, Toshifumi.

In: Journal of Gastroenterology, Vol. 47, No. 9, 09.2012, p. 969-977.

Research output: Contribution to journalArticle

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abstract = "Background The development of a supportive diagnostic method has long been required to differentially diagnose ulcerative colitis (UC) and Crohn's disease (CD). Several antibodies circulate in the sera of patients with inflammatory bowel disease. We previously identified the high mobility group box 1 and box 2 non-histone chromosomal proteins (HMGB1 and HMGB2) as novel antigens of perinuclear type anti-neutrophil cytoplasmic antibodies (pANCA) and discovered anti-HMGB1/HMGB2 antibodies in sera from patients with UC. Here, we evaluated the ability of anti-HMGB1/HMGB2 antibodies combined with anti-Saccharomyces cerevisiae antibodies (ASCA) to differentially diagnose UC and CD. Methods We measured titers of anti-HMGB1/HMGB2 antibodies and ASCA in the sera of 213 patients with UC and 93 with CD, using enzyme-linked immunosorbent assays. Results Among the patients with UC, 26.8{\%} were positive for anti-HMGB1/HMGB2 antibodies, with 85.0{\%} specificity towards CD and a positive predictive value of 80.3{\%}. Corticosteroids significantly suppressed the titer of anti- HMGB1/HMGB2 antibodies. Among the patients with CD, 24.7{\%} were positive for ASCA, with 96.2{\%} specificity towards UC and a positive predictive value of 74.2{\%}. Interestingly, the positivity rate of anti-HMGB/HMGB2 antibodies was higher (35.7{\%}) in patients with the ileitis type ofCDthan in patients withCDin the colon (6.2{\%}; significant difference, P\0.01). The specificity of anti-HMGB1/ HMGB2 antibodies in UC for CD in the colon was 93.8{\%}. Conclusions CD in the colon and UC can be differentially diagnosed using anti-HMGB/HMGB2 antibodies combined with ASCA.",
keywords = "Anti-high mobility group box 1 and box 2 non-histone chromosomal proteins, Anti-Saccharomyces cerevisiae antibodies, Crohn's disease, Ulcerative colitis",
author = "Hiromasa Takaishi and Takanori Kanai and Atsushi Nakazawa and Fumihiko Sugata and Akira Nikai and Shigeo Yoshizawa and Yasuo Hamamoto and Shinsuke Funakoshi and Tomoharu Yajima and Yasushi Iwao and Masao Takemura and Shoichi Ozaki and Toshifumi Hibi",
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T1 - Anti-high mobility group box 1 and box 2 non-histone chromosomal proteins (HMGB1/HMGB2) antibodies and anti-Saccharomyces cerevisiae antibodies (ASCA)

T2 - Accuracy in differentially diagnosing UC and CD and correlation with inflammatory bowel disease phenotype

AU - Takaishi, Hiromasa

AU - Kanai, Takanori

AU - Nakazawa, Atsushi

AU - Sugata, Fumihiko

AU - Nikai, Akira

AU - Yoshizawa, Shigeo

AU - Hamamoto, Yasuo

AU - Funakoshi, Shinsuke

AU - Yajima, Tomoharu

AU - Iwao, Yasushi

AU - Takemura, Masao

AU - Ozaki, Shoichi

AU - Hibi, Toshifumi

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N2 - Background The development of a supportive diagnostic method has long been required to differentially diagnose ulcerative colitis (UC) and Crohn's disease (CD). Several antibodies circulate in the sera of patients with inflammatory bowel disease. We previously identified the high mobility group box 1 and box 2 non-histone chromosomal proteins (HMGB1 and HMGB2) as novel antigens of perinuclear type anti-neutrophil cytoplasmic antibodies (pANCA) and discovered anti-HMGB1/HMGB2 antibodies in sera from patients with UC. Here, we evaluated the ability of anti-HMGB1/HMGB2 antibodies combined with anti-Saccharomyces cerevisiae antibodies (ASCA) to differentially diagnose UC and CD. Methods We measured titers of anti-HMGB1/HMGB2 antibodies and ASCA in the sera of 213 patients with UC and 93 with CD, using enzyme-linked immunosorbent assays. Results Among the patients with UC, 26.8% were positive for anti-HMGB1/HMGB2 antibodies, with 85.0% specificity towards CD and a positive predictive value of 80.3%. Corticosteroids significantly suppressed the titer of anti- HMGB1/HMGB2 antibodies. Among the patients with CD, 24.7% were positive for ASCA, with 96.2% specificity towards UC and a positive predictive value of 74.2%. Interestingly, the positivity rate of anti-HMGB/HMGB2 antibodies was higher (35.7%) in patients with the ileitis type ofCDthan in patients withCDin the colon (6.2%; significant difference, P\0.01). The specificity of anti-HMGB1/ HMGB2 antibodies in UC for CD in the colon was 93.8%. Conclusions CD in the colon and UC can be differentially diagnosed using anti-HMGB/HMGB2 antibodies combined with ASCA.

AB - Background The development of a supportive diagnostic method has long been required to differentially diagnose ulcerative colitis (UC) and Crohn's disease (CD). Several antibodies circulate in the sera of patients with inflammatory bowel disease. We previously identified the high mobility group box 1 and box 2 non-histone chromosomal proteins (HMGB1 and HMGB2) as novel antigens of perinuclear type anti-neutrophil cytoplasmic antibodies (pANCA) and discovered anti-HMGB1/HMGB2 antibodies in sera from patients with UC. Here, we evaluated the ability of anti-HMGB1/HMGB2 antibodies combined with anti-Saccharomyces cerevisiae antibodies (ASCA) to differentially diagnose UC and CD. Methods We measured titers of anti-HMGB1/HMGB2 antibodies and ASCA in the sera of 213 patients with UC and 93 with CD, using enzyme-linked immunosorbent assays. Results Among the patients with UC, 26.8% were positive for anti-HMGB1/HMGB2 antibodies, with 85.0% specificity towards CD and a positive predictive value of 80.3%. Corticosteroids significantly suppressed the titer of anti- HMGB1/HMGB2 antibodies. Among the patients with CD, 24.7% were positive for ASCA, with 96.2% specificity towards UC and a positive predictive value of 74.2%. Interestingly, the positivity rate of anti-HMGB/HMGB2 antibodies was higher (35.7%) in patients with the ileitis type ofCDthan in patients withCDin the colon (6.2%; significant difference, P\0.01). The specificity of anti-HMGB1/ HMGB2 antibodies in UC for CD in the colon was 93.8%. Conclusions CD in the colon and UC can be differentially diagnosed using anti-HMGB/HMGB2 antibodies combined with ASCA.

KW - Anti-high mobility group box 1 and box 2 non-histone chromosomal proteins

KW - Anti-Saccharomyces cerevisiae antibodies

KW - Crohn's disease

KW - Ulcerative colitis

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