Anti-KS: Identification of autoantibodies to asparaginyl-transfer RNA synthetase associated with interstitial lung disease

Michito Hirakata, Akira Suwa, Sonoko Nagai, Michael A. Kron, Edward P. Trieu, Tsuneyo Mimori, Masashi Akizuki, Ira N. Targoff

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142 Citations (Scopus)

Abstract

Autoantibodies to five of the aminoacyl-transfer RNA (tRNA) synthetases have been described, and each is-associated with a syndrome of inflammatory myopathy with interstitial lung disease (ILD) and arthritis. Serum KS, from a patient with ILD and inflammatory arthritis without evidence of myositis, immunoprecipitated a tRNA that was distinct from that precipitated by any described anti-synthetase or other reported tRNA-related Abs, along with a protein of 65 kDa. KS serum and IgG fraction each showed significant (88%) inhibition of asparaginyl-tRNA synthetase (AsnRS) activity, but not of any of the other 19 aminoacyl-tRNA synthetase activities. Among 884 patients with connective tissue diseases tested, only two other sera were found to immunoprecipitate tRNAs and proteins of identical gel mobility. These two and KS showed identical immunodiffusion lines using HeLa cell extract. The new sera significantly inhibited AsnRS without significant effects on other synthetases tested. Both patients had ILD but neither had evidence of myositis. These data strongly suggest that these three sera have autoantibodies to AsnRS, representing a sixth anti-synthetase. Anti-KS was more closely associated with ILD than with myositis. Further study of this Abs might prove useful in dissecting the stimuli responsible for the genesis of anti-synthetase autoantibodies.

Original languageEnglish
Pages (from-to)2315-2320
Number of pages6
JournalJournal of Immunology
Volume162
Issue number4
Publication statusPublished - 1999 Feb 15

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Hirakata, M., Suwa, A., Nagai, S., Kron, M. A., Trieu, E. P., Mimori, T., Akizuki, M., & Targoff, I. N. (1999). Anti-KS: Identification of autoantibodies to asparaginyl-transfer RNA synthetase associated with interstitial lung disease. Journal of Immunology, 162(4), 2315-2320.